STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE
The purpose of the research is to study the immunotoxic properties of the supramolecular complex of fenbendazole (CMF).Materials and methods. The study of the immunotoxic properties of SMF was performed using the agglutination reaction and delayed-type hypersensitivity. In each experimental group, t...
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Federal Scientific Centre VIEV
2019-09-01
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| Series: | Российский паразитологический журнал |
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| Online Access: | https://vniigis.elpub.ru/jour/article/view/366 |
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| author | A. I. Varlamova K. G. Kurochkina N. V. Danilevskaya |
| author_facet | A. I. Varlamova K. G. Kurochkina N. V. Danilevskaya |
| author_sort | A. I. Varlamova |
| collection | DOAJ |
| description | The purpose of the research is to study the immunotoxic properties of the supramolecular complex of fenbendazole (CMF).Materials and methods. The study of the immunotoxic properties of SMF was performed using the agglutination reaction and delayed-type hypersensitivity. In each experimental group, there were 7–8 CBA × C57BL / 6 mice weighing 16–18 g. The CMF was administered once intragastrically at a therapeutic dose of 20 mg/kg for the active substance, in a tenfold dose – 200 mg/kg, the basic drug – fenbendazol asked in a tenfold dose of 20 mg/kg. Control groups of animals received starch paste. Then the mice were immunized intraperitoneally with a 3 % suspension of sheep erythrocytes (test antigen) and distributed into 8 groups. The effect of drugs on antibody production was determined in the hemagglutination reaction, the antibody titer in the serum in the microvariant of the direct hemagglutination reaction with the calculation of the reaction index. The effect of the drug on cellular immunity was established in a delayed-type hypersensitivity reaction (DTH) to sheep erythrocytes.Results and discussion. The introduction of the tested drugs had no inhibitory effect on antibody production in mice of the experimental groups: 6.5±0.22; 6.17±0.47 and 7.75±0.25 (log2) compared with the control – 7.67±0.21 (log2). Reaction indices were respectively 0.85; 0,80 and 1,01. Oral administration of a single CMF in therapeutic (20 mg/kg) and in a tenfold therapeutic dose (200 mg/kg) did not affect the cellular level of immunity. The shifts in the inflammatory response index in animals were 9.05±3.47 and 10.05±2.25% respectively. The introduction of the basic drug stimulated the reaction of HRT, which was manifested by an increase in the inflammation index (21.06±3.32%) compared with the control group (9.27±3.91%). |
| format | Article |
| id | doaj-art-d8273553d26f4ee7b3980bef13b05e82 |
| institution | Kabale University |
| issn | 1998-8435 2541-7843 |
| language | Russian |
| publishDate | 2019-09-01 |
| publisher | Federal Scientific Centre VIEV |
| record_format | Article |
| series | Российский паразитологический журнал |
| spelling | doaj-art-d8273553d26f4ee7b3980bef13b05e822025-08-20T03:59:17ZrusFederal Scientific Centre VIEVРоссийский паразитологический журнал1998-84352541-78432019-09-01132444910.31016/1998-8435-2019-13-2-44-49363STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLEA. I. Varlamova0K. G. Kurochkina1N. V. Danilevskaya2All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants – a branch of Federal State Budgetary Institution of Science "Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences"; Moscow State Academy of Veterinary Medicine and Biotechnology – K. I. Skryabin MBAAll-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants – a branch of Federal State Budgetary Institution of Science "Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences"Moscow State Academy of Veterinary Medicine and Biotechnology – K. I. Skryabin MBAThe purpose of the research is to study the immunotoxic properties of the supramolecular complex of fenbendazole (CMF).Materials and methods. The study of the immunotoxic properties of SMF was performed using the agglutination reaction and delayed-type hypersensitivity. In each experimental group, there were 7–8 CBA × C57BL / 6 mice weighing 16–18 g. The CMF was administered once intragastrically at a therapeutic dose of 20 mg/kg for the active substance, in a tenfold dose – 200 mg/kg, the basic drug – fenbendazol asked in a tenfold dose of 20 mg/kg. Control groups of animals received starch paste. Then the mice were immunized intraperitoneally with a 3 % suspension of sheep erythrocytes (test antigen) and distributed into 8 groups. The effect of drugs on antibody production was determined in the hemagglutination reaction, the antibody titer in the serum in the microvariant of the direct hemagglutination reaction with the calculation of the reaction index. The effect of the drug on cellular immunity was established in a delayed-type hypersensitivity reaction (DTH) to sheep erythrocytes.Results and discussion. The introduction of the tested drugs had no inhibitory effect on antibody production in mice of the experimental groups: 6.5±0.22; 6.17±0.47 and 7.75±0.25 (log2) compared with the control – 7.67±0.21 (log2). Reaction indices were respectively 0.85; 0,80 and 1,01. Oral administration of a single CMF in therapeutic (20 mg/kg) and in a tenfold therapeutic dose (200 mg/kg) did not affect the cellular level of immunity. The shifts in the inflammatory response index in animals were 9.05±3.47 and 10.05±2.25% respectively. The introduction of the basic drug stimulated the reaction of HRT, which was manifested by an increase in the inflammation index (21.06±3.32%) compared with the control group (9.27±3.91%).https://vniigis.elpub.ru/jour/article/view/366supramolecular complexfenbendazolepolyvinylpyrrolidonesheep erythrocytescellular and humoral immune responsehemagglutinationdelayed-type hypersensitivitymice |
| spellingShingle | A. I. Varlamova K. G. Kurochkina N. V. Danilevskaya STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE Российский паразитологический журнал supramolecular complex fenbendazole polyvinylpyrrolidone sheep erythrocytes cellular and humoral immune response hemagglutination delayed-type hypersensitivity mice |
| title | STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE |
| title_full | STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE |
| title_fullStr | STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE |
| title_full_unstemmed | STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE |
| title_short | STUDY OF THE IMMUNOTROPIC ACTIVITY OF THE SUPRAMOLECULAR COMPLEX OF FENBENDAZOLE |
| title_sort | study of the immunotropic activity of the supramolecular complex of fenbendazole |
| topic | supramolecular complex fenbendazole polyvinylpyrrolidone sheep erythrocytes cellular and humoral immune response hemagglutination delayed-type hypersensitivity mice |
| url | https://vniigis.elpub.ru/jour/article/view/366 |
| work_keys_str_mv | AT aivarlamova studyoftheimmunotropicactivityofthesupramolecularcomplexoffenbendazole AT kgkurochkina studyoftheimmunotropicactivityofthesupramolecularcomplexoffenbendazole AT nvdanilevskaya studyoftheimmunotropicactivityofthesupramolecularcomplexoffenbendazole |