The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency
Abstract Essential to life on Earth, assessment of marine photosynthesis is of paramount importance. Photosynthesis occurs in spatially discrete microscopic entities at various levels of biological organization, from subcellular chloroplasts to symbiotic microalgae and macroalgae, and is influenced ...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-08-01
|
| Series: | Methods in Ecology and Evolution |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/2041-210X.70078 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849236683638177792 |
|---|---|
| author | Or Ben‐Zvi Paul Roberts Devin Ratelle Joseph Snider Pichaya Lertvilai Daniel Wangpraseurt Dimitri D. Deheyn Jennifer E. Smith Jules S. Jaffe |
| author_facet | Or Ben‐Zvi Paul Roberts Devin Ratelle Joseph Snider Pichaya Lertvilai Daniel Wangpraseurt Dimitri D. Deheyn Jennifer E. Smith Jules S. Jaffe |
| author_sort | Or Ben‐Zvi |
| collection | DOAJ |
| description | Abstract Essential to life on Earth, assessment of marine photosynthesis is of paramount importance. Photosynthesis occurs in spatially discrete microscopic entities at various levels of biological organization, from subcellular chloroplasts to symbiotic microalgae and macroalgae, and is influenced by the surrounding conditions. As such, in situ photosynthetic efficiency mapping on appropriate scales holds great promise for learning about these processes. To achieve this goal, we designed, fabricated, and tested an underwater microscope that incorporates standard colour, epifluorescence, and variable chlorophyll a fluorescence imaging with nearly micron spatial resolution that resolves the structure and photosynthetic efficiency of benthic organisms. Our results highlight coral observations with high‐resolution photosynthetic spatial variability and detailed morphology. Our imaging system therefore enables research never before possible on the health and physiology of benthic aquatic organisms in situ, placing it in the context of their physical and biological environment. |
| format | Article |
| id | doaj-art-d80d82ec471c4956852d37ff19987b9f |
| institution | Kabale University |
| issn | 2041-210X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Methods in Ecology and Evolution |
| spelling | doaj-art-d80d82ec471c4956852d37ff19987b9f2025-08-20T04:02:09ZengWileyMethods in Ecology and Evolution2041-210X2025-08-011681617162410.1111/2041-210X.70078The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiencyOr Ben‐Zvi0Paul Roberts1Devin Ratelle2Joseph Snider3Pichaya Lertvilai4Daniel Wangpraseurt5Dimitri D. Deheyn6Jennifer E. Smith7Jules S. Jaffe8Marine Physical Lab, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Physical Lab, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Physical Lab, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Physical Lab, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Physical Lab, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Biology Research Division, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Biology Research Division, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Biology Research Division, Scripps Institution of Oceanography University of California San Diego La Jolla California USAMarine Physical Lab, Scripps Institution of Oceanography University of California San Diego La Jolla California USAAbstract Essential to life on Earth, assessment of marine photosynthesis is of paramount importance. Photosynthesis occurs in spatially discrete microscopic entities at various levels of biological organization, from subcellular chloroplasts to symbiotic microalgae and macroalgae, and is influenced by the surrounding conditions. As such, in situ photosynthetic efficiency mapping on appropriate scales holds great promise for learning about these processes. To achieve this goal, we designed, fabricated, and tested an underwater microscope that incorporates standard colour, epifluorescence, and variable chlorophyll a fluorescence imaging with nearly micron spatial resolution that resolves the structure and photosynthetic efficiency of benthic organisms. Our results highlight coral observations with high‐resolution photosynthetic spatial variability and detailed morphology. Our imaging system therefore enables research never before possible on the health and physiology of benthic aquatic organisms in situ, placing it in the context of their physical and biological environment.https://doi.org/10.1111/2041-210X.70078chlorophyllcoralsfluorescenceFv/Fmimaging PAMmicroalgae |
| spellingShingle | Or Ben‐Zvi Paul Roberts Devin Ratelle Joseph Snider Pichaya Lertvilai Daniel Wangpraseurt Dimitri D. Deheyn Jennifer E. Smith Jules S. Jaffe The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency Methods in Ecology and Evolution chlorophyll corals fluorescence Fv/Fm imaging PAM microalgae |
| title | The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency |
| title_full | The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency |
| title_fullStr | The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency |
| title_full_unstemmed | The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency |
| title_short | The Benthic Underwater Microscope imaging PAM (BUMP): A non‐invasive tool for in situ assessment of microstructure and photosynthetic efficiency |
| title_sort | benthic underwater microscope imaging pam bump a non invasive tool for in situ assessment of microstructure and photosynthetic efficiency |
| topic | chlorophyll corals fluorescence Fv/Fm imaging PAM microalgae |
| url | https://doi.org/10.1111/2041-210X.70078 |
| work_keys_str_mv | AT orbenzvi thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT paulroberts thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT devinratelle thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT josephsnider thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT pichayalertvilai thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT danielwangpraseurt thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT dimitriddeheyn thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT jenniferesmith thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT julessjaffe thebenthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT orbenzvi benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT paulroberts benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT devinratelle benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT josephsnider benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT pichayalertvilai benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT danielwangpraseurt benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT dimitriddeheyn benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT jenniferesmith benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency AT julessjaffe benthicunderwatermicroscopeimagingpambumpanoninvasivetoolforinsituassessmentofmicrostructureandphotosyntheticefficiency |