Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles
Objectives. The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. Materials and Methods. Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2021/8975948 |
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| author | Sedighe Sadat Hashemi kamangar Houtan Zareian Abbas Bahador Maryam Pourhajibagher Zahra Bashareh Sara Valizadeh |
| author_facet | Sedighe Sadat Hashemi kamangar Houtan Zareian Abbas Bahador Maryam Pourhajibagher Zahra Bashareh Sara Valizadeh |
| author_sort | Sedighe Sadat Hashemi kamangar |
| collection | DOAJ |
| description | Objectives. The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. Materials and Methods. Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and Lactobacillus acidophilus. Biofilm growth was evaluated by determining colony counts. Antimicrobial effect was determined on days 3, 15, and 30 by counting microbial colonies using eluted components test. One-way ANOVA, Tukey HSD tests, t test, and two-way ANOVA were used for statistical analyses (α = 0.05). Results. Biofilm inhibition test showed that fissure sealant containing 1 wt.% chitosan decreased colony counts significantly (P<0.05). Eluted components test with S. mutans and sanguis showed significant decrease in colony counts during the first 15 days in chitosan containing group; however, from day 30, antimicrobial activity decreased noticeably, with no significant difference from control group (P>0.05). Antimicrobial activity against L. acidophilus was maintained in chitosan group up to 30 days, and decrease in colony counts was significant (P<0.05). Conclusion. According to the results of this study, incorporation of 1 wt.% chitosan into fissure sealant induced an antimicrobial activity. Antibacterial effect on L. acidophilus persisted for longer time (30 days) compared to the two other bacterial species (15 days). |
| format | Article |
| id | doaj-art-d80d5c79d3e04eff81349fac92086de6 |
| institution | DOAJ |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-d80d5c79d3e04eff81349fac92086de62025-08-20T03:23:52ZengWileyInternational Journal of Dentistry1687-87281687-87362021-01-01202110.1155/2021/89759488975948Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan NanoparticlesSedighe Sadat Hashemi kamangar0Houtan Zareian1Abbas Bahador2Maryam Pourhajibagher3Zahra Bashareh4Sara Valizadeh5Department of Operative Dentistry, International Campus, Dental School, Tehran University of Medical Sciences, Tehran, IranInternational Campus, Dental School, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, IranDental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, IranInternational Campus, Dental School, Tehran University of Medical Sciences, Tehran, IranDental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, IranObjectives. The present study evaluated the antimicrobial effects of fissure sealants containing chitosan nanoparticles. Materials and Methods. Antibacterial effect of Master Dent fissure sealant alone and after incorporating chitosan nanoparticles was evaluated on Streptococcus mutans, sanguis, and Lactobacillus acidophilus. Biofilm growth was evaluated by determining colony counts. Antimicrobial effect was determined on days 3, 15, and 30 by counting microbial colonies using eluted components test. One-way ANOVA, Tukey HSD tests, t test, and two-way ANOVA were used for statistical analyses (α = 0.05). Results. Biofilm inhibition test showed that fissure sealant containing 1 wt.% chitosan decreased colony counts significantly (P<0.05). Eluted components test with S. mutans and sanguis showed significant decrease in colony counts during the first 15 days in chitosan containing group; however, from day 30, antimicrobial activity decreased noticeably, with no significant difference from control group (P>0.05). Antimicrobial activity against L. acidophilus was maintained in chitosan group up to 30 days, and decrease in colony counts was significant (P<0.05). Conclusion. According to the results of this study, incorporation of 1 wt.% chitosan into fissure sealant induced an antimicrobial activity. Antibacterial effect on L. acidophilus persisted for longer time (30 days) compared to the two other bacterial species (15 days).http://dx.doi.org/10.1155/2021/8975948 |
| spellingShingle | Sedighe Sadat Hashemi kamangar Houtan Zareian Abbas Bahador Maryam Pourhajibagher Zahra Bashareh Sara Valizadeh Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles International Journal of Dentistry |
| title | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
| title_full | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
| title_fullStr | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
| title_full_unstemmed | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
| title_short | Evaluation of Antibacterial Effects of Fissure Sealants Containing Chitosan Nanoparticles |
| title_sort | evaluation of antibacterial effects of fissure sealants containing chitosan nanoparticles |
| url | http://dx.doi.org/10.1155/2021/8975948 |
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