Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos
In vitro maturation (IVM) of oocyte is an effective procedure for avoiding ovarian hyperstimulation syndrome in patients with polycystic ovaries (PCOS) during in vitro fertilization (IVF). To investigate the influences of IVM on epigenetic reprogramming and to search for the possible reasons for the...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-01-01
|
| Series: | Obstetrics and Gynecology International |
| Online Access: | http://dx.doi.org/10.1155/2010/989278 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849683426597142528 |
|---|---|
| author | Ning Wang Fang Le Qi-Tao Zhan Li Li Min-Yue Dong Guo-Lian Ding Chen-Ming Xu Shi-Wen Jiang He-Feng Huang Fan Jin |
| author_facet | Ning Wang Fang Le Qi-Tao Zhan Li Li Min-Yue Dong Guo-Lian Ding Chen-Ming Xu Shi-Wen Jiang He-Feng Huang Fan Jin |
| author_sort | Ning Wang |
| collection | DOAJ |
| description | In vitro maturation (IVM) of oocyte is an effective procedure for avoiding ovarian hyperstimulation syndrome in patients with polycystic ovaries (PCOS) during in vitro fertilization (IVF). To investigate the influences of IVM on epigenetic reprogramming and to search for the possible reasons for the lower rates of fertilization and cleavage in IVM oocytes, we examined the expression of two enzymes controlling histone acetylation, histone acetyltransferase GCN5 (GCN5) and histone deacetylase 1 (HDAC1), as well as their common target, acetyl-histone H3 (Ac-H3), in mouse metaphase II (MII) oocytes and preimplantation embryos. Results showed that IVM downregulated the protein expression of GCN5 in MII oocytes and two-cell embryos and changed the distribution of GCN5 in two-cell embryos. Expression of HDAC1 mRNA in MII oocytes and two-cell embryos decreased in the IVM group. However, none of these changes persisted after two-cell embryos. Levels of Ac-H3 in both oocytes and embryos remained unchanged after IVM. Our studies indicated that IVM could affect the protein and gene expression related to histone acetylation in oocytes and early cleavage embryos. By function of selection, parts of the changes could be recovered in late embryo development. |
| format | Article |
| id | doaj-art-d80a22074eb04823b0ee7f418ce5a90e |
| institution | DOAJ |
| issn | 1687-9589 1687-9597 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Obstetrics and Gynecology International |
| spelling | doaj-art-d80a22074eb04823b0ee7f418ce5a90e2025-08-20T03:23:52ZengWileyObstetrics and Gynecology International1687-95891687-95972010-01-01201010.1155/2010/989278989278Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage EmbryosNing Wang0Fang Le1Qi-Tao Zhan2Li Li3Min-Yue Dong4Guo-Lian Ding5Chen-Ming Xu6Shi-Wen Jiang7He-Feng Huang8Fan Jin9Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Biomedical Science, Mercer University School of Medicine, Savannah, GA 31499, USADepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaDepartment of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 310006 Hangzhou, ChinaIn vitro maturation (IVM) of oocyte is an effective procedure for avoiding ovarian hyperstimulation syndrome in patients with polycystic ovaries (PCOS) during in vitro fertilization (IVF). To investigate the influences of IVM on epigenetic reprogramming and to search for the possible reasons for the lower rates of fertilization and cleavage in IVM oocytes, we examined the expression of two enzymes controlling histone acetylation, histone acetyltransferase GCN5 (GCN5) and histone deacetylase 1 (HDAC1), as well as their common target, acetyl-histone H3 (Ac-H3), in mouse metaphase II (MII) oocytes and preimplantation embryos. Results showed that IVM downregulated the protein expression of GCN5 in MII oocytes and two-cell embryos and changed the distribution of GCN5 in two-cell embryos. Expression of HDAC1 mRNA in MII oocytes and two-cell embryos decreased in the IVM group. However, none of these changes persisted after two-cell embryos. Levels of Ac-H3 in both oocytes and embryos remained unchanged after IVM. Our studies indicated that IVM could affect the protein and gene expression related to histone acetylation in oocytes and early cleavage embryos. By function of selection, parts of the changes could be recovered in late embryo development.http://dx.doi.org/10.1155/2010/989278 |
| spellingShingle | Ning Wang Fang Le Qi-Tao Zhan Li Li Min-Yue Dong Guo-Lian Ding Chen-Ming Xu Shi-Wen Jiang He-Feng Huang Fan Jin Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos Obstetrics and Gynecology International |
| title | Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos |
| title_full | Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos |
| title_fullStr | Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos |
| title_full_unstemmed | Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos |
| title_short | Effects of In Vitro Maturation on Histone Acetylation in Metaphase II Oocytes and Early Cleavage Embryos |
| title_sort | effects of in vitro maturation on histone acetylation in metaphase ii oocytes and early cleavage embryos |
| url | http://dx.doi.org/10.1155/2010/989278 |
| work_keys_str_mv | AT ningwang effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT fangle effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT qitaozhan effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT lili effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT minyuedong effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT guolianding effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT chenmingxu effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT shiwenjiang effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT hefenghuang effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos AT fanjin effectsofinvitromaturationonhistoneacetylationinmetaphaseiioocytesandearlycleavageembryos |