EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS

Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate...

Full description

Saved in:
Bibliographic Details
Main Authors: Đinh Văn Khiêm, Nguyễn Thị Thu Hậu
Format: Article
Language:English
Published: Dalat University 2018-09-01
Series:Tạp chí Khoa học Đại học Đà Lạt
Subjects:
Online Access:http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832573040252157952
author Đinh Văn Khiêm
Nguyễn Thị Thu Hậu
author_facet Đinh Văn Khiêm
Nguyễn Thị Thu Hậu
author_sort Đinh Văn Khiêm
collection DOAJ
description Leaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate of clean samples was 81.7%. Leaves and stems of the transplanted plants in vitro were cultured on a half dish of MS media supplemented with 30g/l sucrose, 7.5g/l agar and plant growth-regulators: 2.4-Dichlorophenoxyacetic acid (2.4D) and thidiazuron (TDZ). Various concentration of TDZ (0; 0.1; 0.3; 0.5; 1 mg/l) were used. After 21 days, results were obtained for 96% of the callus on the medium supplemented with 0.3 mg/l TDZ. The callus had a fresh weight of 2,642 mg/sample and a dry weight of 271 mg/sample. The calluses were transferred onto a medium for forming shoots and roots to complete the plant in vitro.
format Article
id doaj-art-d802458fdb2343c3a7c3333f5bbf007e
institution Kabale University
issn 0866-787X
0866-787X
language English
publishDate 2018-09-01
publisher Dalat University
record_format Article
series Tạp chí Khoa học Đại học Đà Lạt
spelling doaj-art-d802458fdb2343c3a7c3333f5bbf007e2025-02-02T05:41:26ZengDalat UniversityTạp chí Khoa học Đại học Đà Lạt0866-787X0866-787X2018-09-0183697610.37569/DalatUniversity.8.3.438(2018)258EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUSĐinh Văn Khiêm0Nguyễn Thị Thu Hậu1Viện Nghiên cứu Khoa học Tây NguyênKhoa Nông nghiệp và Phát triển nông thôn, Trường Đại học Kiên GiangLeaves and young petiole of Begonia bataiensis were sterilized with 10% calcium hypochlorite (Ca(OCl)2) and indurations of 0, 4, 6, 8, 10, and 12 minutes. Then they were plated on media consisting of a half dish of MS supplemented by 30 g/l sucrose and 7.5 g/l agar. After 21 days, the culturing rate of clean samples was 81.7%. Leaves and stems of the transplanted plants in vitro were cultured on a half dish of MS media supplemented with 30g/l sucrose, 7.5g/l agar and plant growth-regulators: 2.4-Dichlorophenoxyacetic acid (2.4D) and thidiazuron (TDZ). Various concentration of TDZ (0; 0.1; 0.3; 0.5; 1 mg/l) were used. After 21 days, results were obtained for 96% of the callus on the medium supplemented with 0.3 mg/l TDZ. The callus had a fresh weight of 2,642 mg/sample and a dry weight of 271 mg/sample. The calluses were transferred onto a medium for forming shoots and roots to complete the plant in vitro.http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438cây thu hải đường bataiensis (begonia bataiensis)chất kích thích sinh trưởng thực vậtin vitromô sẹo.
spellingShingle Đinh Văn Khiêm
Nguyễn Thị Thu Hậu
EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
Tạp chí Khoa học Đại học Đà Lạt
cây thu hải đường bataiensis (begonia bataiensis)
chất kích thích sinh trưởng thực vật
in vitro
mô sẹo.
title EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
title_full EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
title_fullStr EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
title_full_unstemmed EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
title_short EFFECT OF PLANT-GROWTH REGULATORS ON THE FORMATION OF Begonia bataiensis CALLUS
title_sort effect of plant growth regulators on the formation of begonia bataiensis callus
topic cây thu hải đường bataiensis (begonia bataiensis)
chất kích thích sinh trưởng thực vật
in vitro
mô sẹo.
url http://tckh.dlu.edu.vn/index.php/tckhdhdl/article/view/438
work_keys_str_mv AT đinhvankhiem effectofplantgrowthregulatorsontheformationofbegoniabataiensiscallus
AT nguyenthithuhau effectofplantgrowthregulatorsontheformationofbegoniabataiensiscallus