Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital
Introduction: Uropathogenic Escherichia coli (UPEC) is a leading cause of community-acquired and healthcare-associated infections, and antimicrobial resistance in UPEC poses significant challenges to managing these infections. This study aimed to investigate the molecular types of UPEC using ent...
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Pasteur Institute of Iran
2024-06-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| Online Access: | https://jommid.pasteur.ac.ir/article-1-630-en.html |
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| author | Amir Sayed Malik1 Mohammed Sameer Chishti1 Gulnaz Bashir1* Irfan Nisar Ahangar1 |
| author_facet | Amir Sayed Malik1 Mohammed Sameer Chishti1 Gulnaz Bashir1* Irfan Nisar Ahangar1 |
| author_sort | Amir Sayed Malik1 |
| collection | DOAJ |
| description | Introduction: Uropathogenic Escherichia coli (UPEC) is a leading cause of
community-acquired and healthcare-associated infections, and antimicrobial
resistance in UPEC poses significant challenges to managing these
infections. This study aimed to investigate the molecular types of UPEC
using enterobacterial repetitive intergenic consensus-polymerase chain
reaction (ERIC-PCR) and analyze their resistance patterns in a tertiary care
setting. Methods: A cross-sectional study was conducted at a tertiary care
hospital, where 65 consecutive E. coli isolates from urinary specimens were
collected. Isolates were identified biochemically and confirmed by 16S
rRNA gene PCR. Antibiotic susceptibility testing was conducted following
CLSI guidelines, and molecular typing was performed using ERIC-PCR.
ERIC-PCR profiles were analyzed using PAST software version 4.0,
generating a dendrogram to visualize similarity among ERIC types. Fisher's
exact test was used to determine if specific ERIC types were significantly
associated with particular antibiotic resistance profiles. Results: The results
showed that 95% of the isolates were resistant to at least two antibiotics, with
92.3% being multidrug resistant (MDR). The highest resistance was
observed against ampicillin, while no resistance was seen against colistin
and tigecycline. The resistant isolates displayed 36 different antibiograms,
indicating a significant degree of resistance variability. ERIC-PCR typing
revealed 22 unique clusters at a similarity coefficient of approximately 70%,
highlighting the genetic diversity of UPEC isolates in our setting.
Conclusion: This study enhances the understanding of UPEC epidemiology
in healthcare by revealing the molecular characteristics and resistance
profiles of prevalent strains. The high occurrence of MDR UPEC and the
absence of a correlation between ERIC types and antibiograms suggest
adaptability and increased resistance. These results highlight the necessity
for continuous surveillance to inform infection control measures and direct
targeted interventions against the spread of MDR UPEC. |
| format | Article |
| id | doaj-art-d7fabf0d075e4ce19f1f434b32e47974 |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-d7fabf0d075e4ce19f1f434b32e479742025-08-20T02:15:28ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302024-06-0112213814910.61186/JoMMID.12.2.138Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care HospitalAmir Sayed Malik10https://orcid.org/0009-0005-9635-4229Mohammed Sameer Chishti11https://orcid.org/0000-0002-0972-5492Gulnaz Bashir1*2https://orcid.org/0000-0002-8048-0854Irfan Nisar Ahangar13https://orcid.org/0009-0007-5277-48401Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Union Territory of Jammu and Kashmir, Pin 190011, India1Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Union Territory of Jammu and Kashmir, Pin 190011, India1Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Union Territory of Jammu and Kashmir, Pin 190011, India1Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Soura, Srinagar, Union Territory of Jammu and Kashmir, Pin 190011, IndiaIntroduction: Uropathogenic Escherichia coli (UPEC) is a leading cause of community-acquired and healthcare-associated infections, and antimicrobial resistance in UPEC poses significant challenges to managing these infections. This study aimed to investigate the molecular types of UPEC using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) and analyze their resistance patterns in a tertiary care setting. Methods: A cross-sectional study was conducted at a tertiary care hospital, where 65 consecutive E. coli isolates from urinary specimens were collected. Isolates were identified biochemically and confirmed by 16S rRNA gene PCR. Antibiotic susceptibility testing was conducted following CLSI guidelines, and molecular typing was performed using ERIC-PCR. ERIC-PCR profiles were analyzed using PAST software version 4.0, generating a dendrogram to visualize similarity among ERIC types. Fisher's exact test was used to determine if specific ERIC types were significantly associated with particular antibiotic resistance profiles. Results: The results showed that 95% of the isolates were resistant to at least two antibiotics, with 92.3% being multidrug resistant (MDR). The highest resistance was observed against ampicillin, while no resistance was seen against colistin and tigecycline. The resistant isolates displayed 36 different antibiograms, indicating a significant degree of resistance variability. ERIC-PCR typing revealed 22 unique clusters at a similarity coefficient of approximately 70%, highlighting the genetic diversity of UPEC isolates in our setting. Conclusion: This study enhances the understanding of UPEC epidemiology in healthcare by revealing the molecular characteristics and resistance profiles of prevalent strains. The high occurrence of MDR UPEC and the absence of a correlation between ERIC types and antibiograms suggest adaptability and increased resistance. These results highlight the necessity for continuous surveillance to inform infection control measures and direct targeted interventions against the spread of MDR UPEC.https://jommid.pasteur.ac.ir/article-1-630-en.htmlupecenterobacterial repetitive intergenic consensuspcrmdreric-pcr typing16s rrna genedisc diffusion methodantibiotypes |
| spellingShingle | Amir Sayed Malik1 Mohammed Sameer Chishti1 Gulnaz Bashir1* Irfan Nisar Ahangar1 Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital Journal of Medical Microbiology and Infectious Diseases upec enterobacterial repetitive intergenic consensus pcr mdr eric-pcr typing 16s rrna gene disc diffusion method antibiotypes |
| title | Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital |
| title_full | Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital |
| title_fullStr | Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital |
| title_full_unstemmed | Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital |
| title_short | Multidrug-Resistant Uropathogenic Escherichia coli Typing by ERIC-PCR: A Genetic and Antibiogram Profiling in a Tertiary Care Hospital |
| title_sort | multidrug resistant uropathogenic escherichia coli typing by eric pcr a genetic and antibiogram profiling in a tertiary care hospital |
| topic | upec enterobacterial repetitive intergenic consensus pcr mdr eric-pcr typing 16s rrna gene disc diffusion method antibiotypes |
| url | https://jommid.pasteur.ac.ir/article-1-630-en.html |
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