B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue
Peizhi Lu,1,2,* Ya Li,1,2,* Shuo Yang,2 Haoyu Yao,2 Bizhi Tu,2 Rende Ning1,2 1Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China; 2Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of H...
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Dove Medical Press
2025-02-01
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| Series: | Journal of Inflammation Research |
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| author | Lu P Li Y Yang S Yao H Tu B Ning R |
| author_facet | Lu P Li Y Yang S Yao H Tu B Ning R |
| author_sort | Lu P |
| collection | DOAJ |
| description | Peizhi Lu,1,2,* Ya Li,1,2,* Shuo Yang,2 Haoyu Yao,2 Bizhi Tu,2 Rende Ning1,2 1Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China; 2Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of Hefei, Hefei, Anhui, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bizhi Tu, Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of Hefei, Hefei, Anhui, People’s Republic of China, Tel +86 15357483840, Email 1301535636@qq.com Rende Ning, Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China, Tel +86 13956966305, Email nrd1972@outlook.comObjective: The objective of this study was to characterize the activation and differentiation of B cells in the synovium of osteoarthritis (OA) and to explore the underlying molecular mechanisms.Methods: Peripheral blood and synovial samples from OA patients at different stages were collected, and flow cytometry was employed to analyze the activation and differentiation of B cells. Immunofluorescence staining of joint synovium from OA mice at different stages was conducted to assess mice joint synovium B cell activation and differentiation. Co-culture experiments of synovial fibroblasts with B cells were performed to investigate the influence of synovial cells on B cell activation and differentiation. Finally, transcriptome analysis was utilized to identify potential key molecules and pathways.Results: In OA patients, the infiltration, activation, and differentiation of B cells in synovium and peripheral blood exhibited distinct characteristics. Specifically, the proportion of activated CD86+ B cells and the differentiation marker HLA-DR+ increased with disease severity, whereas the proportion of the differentiation marker IgM decreased. The proportion of CD38+ B cells also decreased with increasing severity, although this change lacked statistical significance. Immunofluorescence staining of CD19+ and CD86+ cells in mice indicated increased expression with greater OA severity. Co-culture experiments demonstrated that OA synovial fibroblasts promoted B cell activation and differentiation, as evidenced by higher expression levels of CD86+ and HLA-DR+ in the OA group compared to controls. Additionally, the proportion of naive B cells decreased as disease severity progressed.Conclusion: Synovial fibroblasts in OA have been shown to promote the differentiation and activation of B cells, indicating that B cells play a significant role in the pathogenesis of synovium inflammation in OA.Keywords: osteoarthritis, B cells, inflammation, immune, transcriptome sequencing, synovium |
| format | Article |
| id | doaj-art-d7f83f96462a4d9b8438a2abe2bf0af6 |
| institution | Kabale University |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Dove Medical Press |
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| series | Journal of Inflammation Research |
| spelling | doaj-art-d7f83f96462a4d9b8438a2abe2bf0af62025-02-11T17:30:56ZengDove Medical PressJournal of Inflammation Research1178-70312025-02-01Volume 1821372151100077B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial TissueLu PLi YYang SYao HTu BNing RPeizhi Lu,1,2,* Ya Li,1,2,* Shuo Yang,2 Haoyu Yao,2 Bizhi Tu,2 Rende Ning1,2 1Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China; 2Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of Hefei, Hefei, Anhui, People’s Republic of China*These authors contributed equally to this workCorrespondence: Bizhi Tu, Department of Orthopedics, the Third Affiliated Hospital of Anhui Medical University, the First People’s Hospital of Hefei, Hefei, Anhui, People’s Republic of China, Tel +86 15357483840, Email 1301535636@qq.com Rende Ning, Graduate School, Bengbu Medical University, Bengbu, Anhui, People’s Republic of China, Tel +86 13956966305, Email nrd1972@outlook.comObjective: The objective of this study was to characterize the activation and differentiation of B cells in the synovium of osteoarthritis (OA) and to explore the underlying molecular mechanisms.Methods: Peripheral blood and synovial samples from OA patients at different stages were collected, and flow cytometry was employed to analyze the activation and differentiation of B cells. Immunofluorescence staining of joint synovium from OA mice at different stages was conducted to assess mice joint synovium B cell activation and differentiation. Co-culture experiments of synovial fibroblasts with B cells were performed to investigate the influence of synovial cells on B cell activation and differentiation. Finally, transcriptome analysis was utilized to identify potential key molecules and pathways.Results: In OA patients, the infiltration, activation, and differentiation of B cells in synovium and peripheral blood exhibited distinct characteristics. Specifically, the proportion of activated CD86+ B cells and the differentiation marker HLA-DR+ increased with disease severity, whereas the proportion of the differentiation marker IgM decreased. The proportion of CD38+ B cells also decreased with increasing severity, although this change lacked statistical significance. Immunofluorescence staining of CD19+ and CD86+ cells in mice indicated increased expression with greater OA severity. Co-culture experiments demonstrated that OA synovial fibroblasts promoted B cell activation and differentiation, as evidenced by higher expression levels of CD86+ and HLA-DR+ in the OA group compared to controls. Additionally, the proportion of naive B cells decreased as disease severity progressed.Conclusion: Synovial fibroblasts in OA have been shown to promote the differentiation and activation of B cells, indicating that B cells play a significant role in the pathogenesis of synovium inflammation in OA.Keywords: osteoarthritis, B cells, inflammation, immune, transcriptome sequencing, synoviumhttps://www.dovepress.com/b-cell-activation-differentiation-and-their-potential-molecular-mechan-peer-reviewed-fulltext-article-JIRosteoarthritisb cellsinflammationimmunetranscriptome sequencingsynovium |
| spellingShingle | Lu P Li Y Yang S Yao H Tu B Ning R B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue Journal of Inflammation Research osteoarthritis b cells inflammation immune transcriptome sequencing synovium |
| title | B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue |
| title_full | B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue |
| title_fullStr | B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue |
| title_full_unstemmed | B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue |
| title_short | B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial Tissue |
| title_sort | b cell activation differentiation and their potential molecular mechanisms in osteoarthritic synovial tissue |
| topic | osteoarthritis b cells inflammation immune transcriptome sequencing synovium |
| url | https://www.dovepress.com/b-cell-activation-differentiation-and-their-potential-molecular-mechan-peer-reviewed-fulltext-article-JIR |
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