Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes
Progressive individual motility is the main parameter used to determine the reproductive potential of a buffalo bull. This information contributes to deciding to use and process or not the ejaculate obtained and therefore assume that the spermatozoa from the seminal sample could achieve pregnancy i...
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Universidad del Zulia
2023-11-01
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| Online Access: | https://produccioncientificaluz.org/index.php/cientifica/article/view/43458 |
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| author | Armando Quintero-Moreno Carla Osorio Meléndez Héctor Nava-Trujillo Jorge Rubio Guillén Juan Carlos Gutiérrez-Añez Adriana Camacho de Gutiérrez |
| author_facet | Armando Quintero-Moreno Carla Osorio Meléndez Héctor Nava-Trujillo Jorge Rubio Guillén Juan Carlos Gutiérrez-Añez Adriana Camacho de Gutiérrez |
| author_sort | Armando Quintero-Moreno |
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Progressive individual motility is the main parameter used to determine the reproductive potential of a buffalo bull. This information contributes to deciding to use and process or not the ejaculate obtained and therefore assume that the spermatozoa from the seminal sample could achieve pregnancy in inseminated buffaloes. The association of sperm motility to fertility and/or pregnancy rate (PR) has yielded controversial results. To contribute to knowledge in buffalo reproduction, an experiment was carried out where four sperm motility descriptors (VCL: curvilinear velocity, VSL: straight-line velocity, VAP: average path velocity, and LIN: linearity) were evaluated using a Computerized Semen Analysis System (Sperm Class Analyzer®, CASA System, Barcelona, Spain) in samples obtained from 8 frozen-thawed straws of 8 Mediterranean buffaloes. The PR data for each buffalo bull were obtained from 2 farms where the management and FTAI protocol employed was handled by the same professionals and straws of similar provenance and proper nitrogen thermos management were used. The PR data for each buffalo bull was confirmed by ultrasonography, organized in Excel® and analyzed with SAS®, associating VAP to PR, using a Chi-square analysis. To achieve the objective, the PR obtained was categorized from highest to the lowest value (A: PR>70%, B: PR>60≤70%, C: PR>50≤60%, D: PR>40≤50%), in addition to creating sperm subpopulations (Sp) based on the quality of sperm movement, for which it was decided to use the VAP, since it is a variable that integrates the VCL and the VSL and indirectly assumes the LIN, creating the following categories: Z= Sp with VAP value ≥ 80 mμ/s, Y: Sp with a VAP value ≥ 50 and < 80 mμ/s, X= Sp with a VAP ≥30 and < 50 mμ/s, W=Sp with a VAP ≥ 10 <30 mμ/s, V= VAP <10 mμ/s. The SAS® GLM procedure was used to assess the VAP velocity according to the fertility rate obtained. The results show that the buffalo bulls with high fertility (A and B) presented in their ejaculates sperm with the best VAP (Z: 14.6% and Y: 15.84%) and less quantity of immobile sperm (W: 7.14% and V: 1.63%) (P<0.01). In contrast, the buffalo bulls with lower fertility (C and D) had a considerable decrease in sperm with high velocity (Z: 4.84%) or immobile (6.24%) (p>0.01). There is an evident association between the average speed (VAP) provided by the CASA system and the PR in this species. This type of analysis provides the opportunity to quantify sperm movements and group them to generate more accurate sperm motility values, which allows the prediction of the fertility and/or PR in this species.
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| format | Article |
| id | doaj-art-d7f52257a7d0427e91b7cfa736c1eec7 |
| institution | Kabale University |
| issn | 0798-2259 2521-9715 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universidad del Zulia |
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| series | Revista Científica |
| spelling | doaj-art-d7f52257a7d0427e91b7cfa736c1eec72025-02-05T15:37:05ZengUniversidad del ZuliaRevista Científica0798-22592521-97152023-11-0133Suplemento10.52973/rcfcv-wbc112Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloesArmando Quintero-Moreno0Carla Osorio Meléndez 1Héctor Nava-Trujillo2Jorge Rubio Guillén 3Juan Carlos Gutiérrez-Añez4Adriana Camacho de Gutiérrez 5Department of Physiology, Faculty of Veterinary Medicine, University of Murcia, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, SpainAndrology Laboratory. Faculty of Veterinary Sciences (FCV). University of Zulia (LUZ). VenezuelaDivision of Animal Sciences, University of Missouri, Columbia MO, USAAndrology Laboratory. Faculty of Veterinary Sciences (FCV). University of Zulia (LUZ). VenezuelaBIOTERVET (Biotecnología Reproductiva Veterinaria, C.A), Maracaibo, VenezuelaBIOTERVET (Biotecnología Reproductiva Veterinaria, C.A), Maracaibo, Venezuela Progressive individual motility is the main parameter used to determine the reproductive potential of a buffalo bull. This information contributes to deciding to use and process or not the ejaculate obtained and therefore assume that the spermatozoa from the seminal sample could achieve pregnancy in inseminated buffaloes. The association of sperm motility to fertility and/or pregnancy rate (PR) has yielded controversial results. To contribute to knowledge in buffalo reproduction, an experiment was carried out where four sperm motility descriptors (VCL: curvilinear velocity, VSL: straight-line velocity, VAP: average path velocity, and LIN: linearity) were evaluated using a Computerized Semen Analysis System (Sperm Class Analyzer®, CASA System, Barcelona, Spain) in samples obtained from 8 frozen-thawed straws of 8 Mediterranean buffaloes. The PR data for each buffalo bull were obtained from 2 farms where the management and FTAI protocol employed was handled by the same professionals and straws of similar provenance and proper nitrogen thermos management were used. The PR data for each buffalo bull was confirmed by ultrasonography, organized in Excel® and analyzed with SAS®, associating VAP to PR, using a Chi-square analysis. To achieve the objective, the PR obtained was categorized from highest to the lowest value (A: PR>70%, B: PR>60≤70%, C: PR>50≤60%, D: PR>40≤50%), in addition to creating sperm subpopulations (Sp) based on the quality of sperm movement, for which it was decided to use the VAP, since it is a variable that integrates the VCL and the VSL and indirectly assumes the LIN, creating the following categories: Z= Sp with VAP value ≥ 80 mμ/s, Y: Sp with a VAP value ≥ 50 and < 80 mμ/s, X= Sp with a VAP ≥30 and < 50 mμ/s, W=Sp with a VAP ≥ 10 <30 mμ/s, V= VAP <10 mμ/s. The SAS® GLM procedure was used to assess the VAP velocity according to the fertility rate obtained. The results show that the buffalo bulls with high fertility (A and B) presented in their ejaculates sperm with the best VAP (Z: 14.6% and Y: 15.84%) and less quantity of immobile sperm (W: 7.14% and V: 1.63%) (P<0.01). In contrast, the buffalo bulls with lower fertility (C and D) had a considerable decrease in sperm with high velocity (Z: 4.84%) or immobile (6.24%) (p>0.01). There is an evident association between the average speed (VAP) provided by the CASA system and the PR in this species. This type of analysis provides the opportunity to quantify sperm movements and group them to generate more accurate sperm motility values, which allows the prediction of the fertility and/or PR in this species. https://produccioncientificaluz.org/index.php/cientifica/article/view/43458sperm motilityCASA systempregnancy rate |
| spellingShingle | Armando Quintero-Moreno Carla Osorio Meléndez Héctor Nava-Trujillo Jorge Rubio Guillén Juan Carlos Gutiérrez-Añez Adriana Camacho de Gutiérrez Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes Revista Científica sperm motility CASA system pregnancy rate |
| title | Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes |
| title_full | Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes |
| title_fullStr | Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes |
| title_full_unstemmed | Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes |
| title_short | Association between sperm motility in thawed semen and pregnancy rate in Mediterranean buffaloes |
| title_sort | association between sperm motility in thawed semen and pregnancy rate in mediterranean buffaloes |
| topic | sperm motility CASA system pregnancy rate |
| url | https://produccioncientificaluz.org/index.php/cientifica/article/view/43458 |
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