Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer

Abstract Topoisomerases are critical nuclear enzymes that resolve DNA topological challenges during genetic processes. However, there is currently a lack of comprehensive multi-omics analysis of TOP2A from a pan-cancer perspective, despite its significance. A multiomics analysis was conducted to inv...

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Main Authors: Jin Zhang, Tianxiao Yang, Kenie Wang, Jie Pan, Junjun Qiu, Shengnai Zheng, Xuesong Li, Guanghao Li
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-85929-9
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author Jin Zhang
Tianxiao Yang
Kenie Wang
Jie Pan
Junjun Qiu
Shengnai Zheng
Xuesong Li
Guanghao Li
author_facet Jin Zhang
Tianxiao Yang
Kenie Wang
Jie Pan
Junjun Qiu
Shengnai Zheng
Xuesong Li
Guanghao Li
author_sort Jin Zhang
collection DOAJ
description Abstract Topoisomerases are critical nuclear enzymes that resolve DNA topological challenges during genetic processes. However, there is currently a lack of comprehensive multi-omics analysis of TOP2A from a pan-cancer perspective, despite its significance. A multiomics analysis was conducted to investigate TOP2A across various cancer types. This study involved the integration of over 10,000 multidimensional genomic datasets from 33 distinct cancer types, obtained from The Cancer Genome Atlas (TCGA). The analysis focused on evaluating the overall activity levels of TOP2A in pan-cancers, which encompassed differential expression, clinical significance, immune cell infiltration, and the regulation of pathways related to cancer. Aberrant epigenetic modifications and genomic alterations have been identified as being associated with the dysregulation of TOP2A expression levels. These molecular changes have substantial impacts on cancer progression, intratumoral heterogeneity, immunological status, and the regulation of pathways related to cancer biomarkers. Consequently, patient prognosis varies significantly based on the presence and specific nature of these alterations. The potential of TOP2A to serve as a novel biomarker for prognosis may offer valuable insights into the diagnosis and treatment of cancer.
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publishDate 2025-02-01
publisher Nature Portfolio
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series Scientific Reports
spelling doaj-art-d7dd9bb8fc8f4484afa1974ccdb001042025-08-20T03:10:57ZengNature PortfolioScientific Reports2045-23222025-02-0115111410.1038/s41598-025-85929-9Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancerJin Zhang0Tianxiao Yang1Kenie Wang2Jie Pan3Junjun Qiu4Shengnai Zheng5Xuesong Li6Guanghao Li7Department of Orthopedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Orthopedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical UniversityThe First Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for CancerDepartment of Spine, Shanghai East HospitalDepartment of Orthopedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Orthopedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical UniversityDepartment of Pediatric Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical SciencesDepartment of Urology, Shandong Cancer Hospital and Institute, Shandong First Medical University, Shandong Academy of Medical SciencesAbstract Topoisomerases are critical nuclear enzymes that resolve DNA topological challenges during genetic processes. However, there is currently a lack of comprehensive multi-omics analysis of TOP2A from a pan-cancer perspective, despite its significance. A multiomics analysis was conducted to investigate TOP2A across various cancer types. This study involved the integration of over 10,000 multidimensional genomic datasets from 33 distinct cancer types, obtained from The Cancer Genome Atlas (TCGA). The analysis focused on evaluating the overall activity levels of TOP2A in pan-cancers, which encompassed differential expression, clinical significance, immune cell infiltration, and the regulation of pathways related to cancer. Aberrant epigenetic modifications and genomic alterations have been identified as being associated with the dysregulation of TOP2A expression levels. These molecular changes have substantial impacts on cancer progression, intratumoral heterogeneity, immunological status, and the regulation of pathways related to cancer biomarkers. Consequently, patient prognosis varies significantly based on the presence and specific nature of these alterations. The potential of TOP2A to serve as a novel biomarker for prognosis may offer valuable insights into the diagnosis and treatment of cancer.https://doi.org/10.1038/s41598-025-85929-9TCGAImmune microenvironmentTOP2AImmune checkpointsImmunotherapy
spellingShingle Jin Zhang
Tianxiao Yang
Kenie Wang
Jie Pan
Junjun Qiu
Shengnai Zheng
Xuesong Li
Guanghao Li
Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer
Scientific Reports
TCGA
Immune microenvironment
TOP2A
Immune checkpoints
Immunotherapy
title Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer
title_full Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer
title_fullStr Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer
title_full_unstemmed Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer
title_short Multi-omics analysis reveals the panoramic picture of TOP2A in pan-cancer
title_sort multi omics analysis reveals the panoramic picture of top2a in pan cancer
topic TCGA
Immune microenvironment
TOP2A
Immune checkpoints
Immunotherapy
url https://doi.org/10.1038/s41598-025-85929-9
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