α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata
Seaweeds have gained interest from the pharmaceutical industry due to their diverse secondary metabolites with potential applications in the prevention and treatment of lifestyle diseases. In spite of the abundance of seaweeds in the coastal area of Iligan Bay in the Philippines, there has been limi...
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Diponegoro University; Association of Indonesian Coastal Management Experts
2023-09-01
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| Series: | Ilmu Kelautan |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ijms/article/view/54721 |
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| author | Kimnard Joseph Magsacay Duat Chona de Guzman Gelani |
| author_facet | Kimnard Joseph Magsacay Duat Chona de Guzman Gelani |
| author_sort | Kimnard Joseph Magsacay Duat |
| collection | DOAJ |
| description | Seaweeds have gained interest from the pharmaceutical industry due to their diverse secondary metabolites with potential applications in the prevention and treatment of lifestyle diseases. In spite of the abundance of seaweeds in the coastal area of Iligan Bay in the Philippines, there has been limited investigation into their pharmacological properties. Therefore, this study aimed to evaluate the in vitro α-glucosidase inhibition, angiotensin-converting enzyme (ACE) inhibition, and acetylcholinesterase (AChE) inhibition properties of various fractions obtained from the marine red alga Galaxaura oblongata. The methanol extract of G. oblongata was sequentially partitioned into hexane, dichloromethane, ethyl acetate, butanol, and methanol fractions. All assays were performed in vitro and microplate based. The results of α-glucosidase inhibition activity assay showed that the n-hexane fraction and dichloromethane fraction exhibited greater than 50% inhibitory activity at 200 ppm. Furthermore, ACE inhibition activity assay revealed that dichloromethane fraction, ethyl acetate fraction, and butanol fraction displayed ACE inhibition activity above 50% at 250 ppm. In addition, the hexane fraction, dichloromethane fraction, and ethyl acetate fraction demonstrated potent AChE inhibitory activity at 250 ppm. Overall, the findings show that G. oblongata has antidiabetic, anti-hypertensive, and neuroprotective potentials. This is the first report of the in vitro α-Glucosidase, ACE, and AChE inhibition activities of G. oblongata. Further investigation and purification of the highly potent fractions in each assay is highly suggested to identify and characterize the compounds responsible for the observed bioactivities, which could serve as possible leads for drug discovery efforts in the management of various lifestyle diseases. |
| format | Article |
| id | doaj-art-d7d6d613024749248bd7ff29a1d7475b |
| institution | DOAJ |
| issn | 0853-7291 2406-7598 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Diponegoro University; Association of Indonesian Coastal Management Experts |
| record_format | Article |
| series | Ilmu Kelautan |
| spelling | doaj-art-d7d6d613024749248bd7ff29a1d7475b2025-08-20T02:43:50ZengDiponegoro University; Association of Indonesian Coastal Management ExpertsIlmu Kelautan0853-72912406-75982023-09-0128322523010.14710/ik.ijms.28.3.225-23022688α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongataKimnard Joseph Magsacay Duat0Chona de Guzman Gelani1Department of Chemistry, College of Science and Mathematics, Mindanao State University, Iligan Institute of Technology, PhilippinesDepartment of Chemistry, College of Science and Mathematics, Mindanao State University, Iligan Institute of Technology, PhilippinesSeaweeds have gained interest from the pharmaceutical industry due to their diverse secondary metabolites with potential applications in the prevention and treatment of lifestyle diseases. In spite of the abundance of seaweeds in the coastal area of Iligan Bay in the Philippines, there has been limited investigation into their pharmacological properties. Therefore, this study aimed to evaluate the in vitro α-glucosidase inhibition, angiotensin-converting enzyme (ACE) inhibition, and acetylcholinesterase (AChE) inhibition properties of various fractions obtained from the marine red alga Galaxaura oblongata. The methanol extract of G. oblongata was sequentially partitioned into hexane, dichloromethane, ethyl acetate, butanol, and methanol fractions. All assays were performed in vitro and microplate based. The results of α-glucosidase inhibition activity assay showed that the n-hexane fraction and dichloromethane fraction exhibited greater than 50% inhibitory activity at 200 ppm. Furthermore, ACE inhibition activity assay revealed that dichloromethane fraction, ethyl acetate fraction, and butanol fraction displayed ACE inhibition activity above 50% at 250 ppm. In addition, the hexane fraction, dichloromethane fraction, and ethyl acetate fraction demonstrated potent AChE inhibitory activity at 250 ppm. Overall, the findings show that G. oblongata has antidiabetic, anti-hypertensive, and neuroprotective potentials. This is the first report of the in vitro α-Glucosidase, ACE, and AChE inhibition activities of G. oblongata. Further investigation and purification of the highly potent fractions in each assay is highly suggested to identify and characterize the compounds responsible for the observed bioactivities, which could serve as possible leads for drug discovery efforts in the management of various lifestyle diseases.https://ejournal.undip.ac.id/index.php/ijms/article/view/54721α-glucosidase inhibitorace inhibitorache inhibitorgalaxaura oblongata |
| spellingShingle | Kimnard Joseph Magsacay Duat Chona de Guzman Gelani α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata Ilmu Kelautan α-glucosidase inhibitor ace inhibitor ache inhibitor galaxaura oblongata |
| title | α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata |
| title_full | α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata |
| title_fullStr | α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata |
| title_full_unstemmed | α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata |
| title_short | α-Glucosidase, Angiotensin-converting Enzyme, and Acetylcholinesterase Inhibitory Activities of a Marine Red Alga Galaxaura oblongata |
| title_sort | α glucosidase angiotensin converting enzyme and acetylcholinesterase inhibitory activities of a marine red alga galaxaura oblongata |
| topic | α-glucosidase inhibitor ace inhibitor ache inhibitor galaxaura oblongata |
| url | https://ejournal.undip.ac.id/index.php/ijms/article/view/54721 |
| work_keys_str_mv | AT kimnardjosephmagsacayduat aglucosidaseangiotensinconvertingenzymeandacetylcholinesteraseinhibitoryactivitiesofamarineredalgagalaxauraoblongata AT chonadeguzmangelani aglucosidaseangiotensinconvertingenzymeandacetylcholinesteraseinhibitoryactivitiesofamarineredalgagalaxauraoblongata |