Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology?
Paratuberculosis (Johne’s disease) has devastating outcomes on ruminant health and impacts on national and international trade. The current work assessed the diagnostic value of the VersaTREK automated liquid culture system in isolating Mycobacterium avium subspecies paratuberculosis (MAP) from faec...
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| Format: | Article |
| Language: | English |
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AOSIS
2025-02-01
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| Series: | Onderstepoort Journal of Veterinary Research |
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| Online Access: | https://ojvr.org/index.php/ojvr/article/view/2159 |
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| author | Motlatso T. Hlokwe Nomawethu S. Masina Boitumelo Letsoko Sewellyn C. Davey Anita L. Michel |
| author_facet | Motlatso T. Hlokwe Nomawethu S. Masina Boitumelo Letsoko Sewellyn C. Davey Anita L. Michel |
| author_sort | Motlatso T. Hlokwe |
| collection | DOAJ |
| description | Paratuberculosis (Johne’s disease) has devastating outcomes on ruminant health and impacts on national and international trade. The current work assessed the diagnostic value of the VersaTREK automated liquid culture system in isolating Mycobacterium avium subspecies paratuberculosis (MAP) from faecal and intestinal tissue samples from ovine under South African conditions and compared it with the current method of choice, histopathological examination. Intestinal tissue and faecal samples from 111 sheep (including complete set from 104 slaughter sheep from flocks with a history of MAP infection as well as incomplete sample sets from 7 sheep) were analysed using the liquid culture method. One set of tissues was subjected to histopathological examination. Deoxyribonucleic acid (DNA) extracted from culture isolates was subjected to polymerase chain reaction (PCR) amplification using primers that target the IS900 regions of the MAP for species verification. Overall, the VersaTREK automated liquid culture in combination with IS900 PCR showed a comparable level of detection in tissues (12.6%) as histopathology (13.5%), but the detection rate for faecal samples was lower than for tissues (10.8%). A combination of histopathology and faecal culture increased the detection rate from 13.5% (n = 14/104) and 9.6% (n = 10/104), respectively, to 15.4% (n = 16/104).
Contribution: Our findings highlight the diagnostic utility of the VersaTREK automated liquid culture system in detecting MAP in ovine samples collected both ante and postmortem. However, an inhibitory effect on the MAP isolation rate observed when the antibiotic cocktail was added to the culture medium warrants further investigation. The outcome of the study is beneficial in guiding the strategic planning of the nationwide control programme. |
| format | Article |
| id | doaj-art-d7cd016c80b54b33a9abae7a97e0abc7 |
| institution | Kabale University |
| issn | 0030-2465 2219-0635 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Onderstepoort Journal of Veterinary Research |
| spelling | doaj-art-d7cd016c80b54b33a9abae7a97e0abc72025-02-11T13:26:15ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352025-02-01921e1e910.4102/ojvr.v92i1.2159723Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology?Motlatso T. Hlokwe0Nomawethu S. Masina1Boitumelo Letsoko2Sewellyn C. Davey3Anita L. Michel4Tuberculosis Laboratory, Diagnostic Services Programme, ARC-Onderstepoort Veterinary Research, PretoriaTuberculosis Laboratory, Diagnostic Services Programme, ARC-Onderstepoort Veterinary Research, Pretoria, South Africa; and, Department of Veterinary Tropical Diseases, Bovine TB and Brucellosis Research Programme, Faculty of Veterinary Sciences, University of Pretoria, PretoriaTuberculosis Laboratory, Diagnostic Services Programme, ARC-Onderstepoort Veterinary Research, Onderstepoort, Pretoria, South Africa; and, Mammalian Serology Laboratory, Potchefstroom Provincial Veterinary Laboratory, PotchefstroomDepartment of Agriculture, Veterinary Services, Animal Health, State Veterinarian Malmesbury, MalmesburyDepartment of Veterinary Tropical Diseases, Bovine TB and Brucellosis Research Programme, Faculty of Veterinary Sciences, University of Pretoria, PretoriaParatuberculosis (Johne’s disease) has devastating outcomes on ruminant health and impacts on national and international trade. The current work assessed the diagnostic value of the VersaTREK automated liquid culture system in isolating Mycobacterium avium subspecies paratuberculosis (MAP) from faecal and intestinal tissue samples from ovine under South African conditions and compared it with the current method of choice, histopathological examination. Intestinal tissue and faecal samples from 111 sheep (including complete set from 104 slaughter sheep from flocks with a history of MAP infection as well as incomplete sample sets from 7 sheep) were analysed using the liquid culture method. One set of tissues was subjected to histopathological examination. Deoxyribonucleic acid (DNA) extracted from culture isolates was subjected to polymerase chain reaction (PCR) amplification using primers that target the IS900 regions of the MAP for species verification. Overall, the VersaTREK automated liquid culture in combination with IS900 PCR showed a comparable level of detection in tissues (12.6%) as histopathology (13.5%), but the detection rate for faecal samples was lower than for tissues (10.8%). A combination of histopathology and faecal culture increased the detection rate from 13.5% (n = 14/104) and 9.6% (n = 10/104), respectively, to 15.4% (n = 16/104). Contribution: Our findings highlight the diagnostic utility of the VersaTREK automated liquid culture system in detecting MAP in ovine samples collected both ante and postmortem. However, an inhibitory effect on the MAP isolation rate observed when the antibiotic cocktail was added to the culture medium warrants further investigation. The outcome of the study is beneficial in guiding the strategic planning of the nationwide control programme.https://ojvr.org/index.php/ojvr/article/view/2159mycobacterium avium subspeciesparatuberculosisovine johne’s diseaseversatrek automated liquid culture systemantibiotic cocktail. |
| spellingShingle | Motlatso T. Hlokwe Nomawethu S. Masina Boitumelo Letsoko Sewellyn C. Davey Anita L. Michel Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? Onderstepoort Journal of Veterinary Research mycobacterium avium subspecies paratuberculosis ovine johne’s disease versatrek automated liquid culture system antibiotic cocktail. |
| title | Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? |
| title_full | Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? |
| title_fullStr | Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? |
| title_full_unstemmed | Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? |
| title_short | Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology? |
| title_sort | are there benefits of culture based detection of mycobacterium avium spp paratuberculosis over histopathology |
| topic | mycobacterium avium subspecies paratuberculosis ovine johne’s disease versatrek automated liquid culture system antibiotic cocktail. |
| url | https://ojvr.org/index.php/ojvr/article/view/2159 |
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