Protocol for purifying biologically active microtubule-severing protein UNC-45A from E.coli using GST-affinity and spin columns

Protocol for purifying biologically active microtubule-severing protein UNC-45A from E.coli using GST-affinity and spin columns

Summary: Recombinant microtubule (MT)-severing proteins are valuable for studying their mechanisms of action; however, purifying them in an active state is challenging. Here, we provide a protocol to obtain biologically active and highly pure recombinant GFP-UNC-45A, a novel ATP-independent MT-sever...

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Bibliographic Details
Main Authors: Asumi Hoshino, Nimisha Krishnan, Mihir Shetty, Jennifer L. Ross, Martina Bazzaro
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Biophysics
Single-molecule Assays
Protein Biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000619
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