Genome-Wide Analysis of GRETCHEN HAGEN3 Genes and Characterization of IAA-Amido Synthetase Gene <i>CsGH3.1</i> in Rhizome Proliferation in <i>Cymbidium sinense</i> ‘Qijianbaimo’
This study systematically identified and functionally analyzed the <i>GRETCHEN HAGEN</i> 3 (<i>GH3</i>) gene family in <i>Cymbidium sinense</i> ‘Qijianbaimo’ (CSQ). Seven <i>GH3</i> genes (<i>CsGH3.1</i>–<i>CsGH3.7</i>) were ide...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/9/1287 |
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| Summary: | This study systematically identified and functionally analyzed the <i>GRETCHEN HAGEN</i> 3 (<i>GH3</i>) gene family in <i>Cymbidium sinense</i> ‘Qijianbaimo’ (CSQ). Seven <i>GH3</i> genes (<i>CsGH3.1</i>–<i>CsGH3.7</i>) were identified using genomic and transcriptomic data. Phylogenetic analysis revealed that CsGH3s are divided into two subfamilies, with CsGH3.1–CsGH3.4 belonging to subfamily II and CsGH3.5–CsGH3.7 to subfamily I. Gene structure analysis showed that <i>CsGH3s</i> contain one to four exons, and their promoter regions feature auxin and jasmonic acid response elements. <i>CsGH3.1</i> and <i>CsGH3.2</i> were significantly upregulated in response to naphthaleneacetic acid (NAA) during rhizome proliferation, indicating their pivotal role in auxin homeostasis regulation. The overexpression of <i>CsGH3.1</i> in <i>Arabidopsis</i> led to restricted growth and a significant reduction in callus proliferation rates, further confirming its role in regulating cell proliferation and auxin homeostasis. This study provides new insights into the function of the <i>CsGH3.1</i> gene in the rhizome proliferation of <i>Cymbidium sinense</i> and lays a foundation for future research on the molecular mechanism of micropropagation in <i>Cymbidium</i>. |
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| ISSN: | 2223-7747 |