Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam.
Visceral leishmaniasis (VL), or kala-azar, is an infection of the reticuloendothelial system, Fatalities due to leishmaniasis are associated with visceral disease. the Leishmaia donovani is a causative agent for this disease. In the current study, white male mice of the Balb /C strain were exper...
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Tikrit University
2023-02-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/904 |
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| author | Maksood A.M. Al-Doori Tawfiq I . Al-Alousi Abdulla H. Al-Jboori |
| author_facet | Maksood A.M. Al-Doori Tawfiq I . Al-Alousi Abdulla H. Al-Jboori |
| author_sort | Maksood A.M. Al-Doori |
| collection | DOAJ |
| description |
Visceral leishmaniasis (VL), or kala-azar, is an infection of the reticuloendothelial system, Fatalities due to leishmaniasis are associated with visceral disease. the Leishmaia donovani is a causative agent for this disease. In the current study, white male mice of the Balb /C strain were experimentally infected with the leishmania donovani promastigotes and were then treated with a Viscum extract and compared with the Pentostam. The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in blood samples of infected mice. as well as, evaluation the ability of Viscum album extract to treatment the visceral leishmaniasis. The genetic study gave excellent results in the diagnosis of infection throughout the treatment period compared to the positive and negative control . Using the specific LdI primer , the 700bp of the parasite was diagnosed. This Broad band was shown in the positive control samples, in the pantostam treatment groups, and the muscular injection with viscum extract in the first 15 days and in the oral dosage group in the first 20 days of initiation. While the band did not appear in any other. The current study has shown promising results in the use of V. album in the treatment of visceral leishmaniasis and also gave impressive results in the diagnosis of infection PCR.
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| format | Article |
| id | doaj-art-d7beab51e16a44bc9d9e28eefcece148 |
| institution | OA Journals |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-d7beab51e16a44bc9d9e28eefcece1482025-08-20T02:36:31ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-02-01221110.25130/tjps.v22i11.904Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam.Maksood A.M. Al-DooriTawfiq I . Al-AlousiAbdulla H. Al-Jboori Visceral leishmaniasis (VL), or kala-azar, is an infection of the reticuloendothelial system, Fatalities due to leishmaniasis are associated with visceral disease. the Leishmaia donovani is a causative agent for this disease. In the current study, white male mice of the Balb /C strain were experimentally infected with the leishmania donovani promastigotes and were then treated with a Viscum extract and compared with the Pentostam. The aim of this study was to evaluate the sensitivity and specificity of polymerase chain reaction (PCR) in the detection of Leishmania DNA in blood samples of infected mice. as well as, evaluation the ability of Viscum album extract to treatment the visceral leishmaniasis. The genetic study gave excellent results in the diagnosis of infection throughout the treatment period compared to the positive and negative control . Using the specific LdI primer , the 700bp of the parasite was diagnosed. This Broad band was shown in the positive control samples, in the pantostam treatment groups, and the muscular injection with viscum extract in the first 15 days and in the oral dosage group in the first 20 days of initiation. While the band did not appear in any other. The current study has shown promising results in the use of V. album in the treatment of visceral leishmaniasis and also gave impressive results in the diagnosis of infection PCR. https://tjpsj.org/index.php/tjps/article/view/904Viscum albumalcohol extractLeishmania donovaniPCR |
| spellingShingle | Maksood A.M. Al-Doori Tawfiq I . Al-Alousi Abdulla H. Al-Jboori Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam. Tikrit Journal of Pure Science Viscum album alcohol extract Leishmania donovani PCR |
| title | Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam. |
| title_full | Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam. |
| title_fullStr | Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam. |
| title_full_unstemmed | Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam. |
| title_short | Diagnostic of visceral leishmaniasis by polymerase chain reaction PCR in experimental mice infected which treatment with Viscum album extract and Pentostam. |
| title_sort | diagnostic of visceral leishmaniasis by polymerase chain reaction pcr in experimental mice infected which treatment with viscum album extract and pentostam |
| topic | Viscum album alcohol extract Leishmania donovani PCR |
| url | https://tjpsj.org/index.php/tjps/article/view/904 |
| work_keys_str_mv | AT maksoodamaldoori diagnosticofvisceralleishmaniasisbypolymerasechainreactionpcrinexperimentalmiceinfectedwhichtreatmentwithviscumalbumextractandpentostam AT tawfiqialalousi diagnosticofvisceralleishmaniasisbypolymerasechainreactionpcrinexperimentalmiceinfectedwhichtreatmentwithviscumalbumextractandpentostam AT abdullahaljboori diagnosticofvisceralleishmaniasisbypolymerasechainreactionpcrinexperimentalmiceinfectedwhichtreatmentwithviscumalbumextractandpentostam |