Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture

This study was aimed to use plant tissue culture technique to induce callus formation of Aloe vera on MS. Medium supplied with 10 mg/l NAA and 5 mg/l BA that exhibit the best results even with subculturing. As the method of [1] 1g. dru weight of callus induced from A. vera crown and in vivo crown we...

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Main Author: Baghdad Science Journal
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2012-03-01
Series:مجلة بغداد للعلوم
Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1328
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author Baghdad Science Journal
author_facet Baghdad Science Journal
author_sort Baghdad Science Journal
collection DOAJ
description This study was aimed to use plant tissue culture technique to induce callus formation of Aloe vera on MS. Medium supplied with 10 mg/l NAA and 5 mg/l BA that exhibit the best results even with subculturing. As the method of [1] 1g. dru weight of callus induced from A. vera crown and in vivo crown were extracted then injected in HPLC using the standards of Ascorbic acid (vit. C), Salysilic acid and Nicotenic acid (vit. B5) to compare with the plant extracts. Results showed high potential of increasing some secondary products using the crown callus culture of A. vera as compared with in vivo crown, Ascorbic acid was 1.829 ?g/l in in vivo crown and increased to 3.905 ?g/l crown callus culture . Salysilic acid raised from 3.54 ?g/l in in vivo crown and reached to 25,487? g/l and the Nicotenic acid was 19.391 mg/l and decreased to 7.438 ?g/l.
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publishDate 2012-03-01
publisher University of Baghdad, College of Science for Women
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spelling doaj-art-d7bcb7b605fa4968bb61ba348109f75a2025-08-20T02:51:14ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862012-03-019110.21123/bsj.9.1.18-22Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus cultureBaghdad Science JournalThis study was aimed to use plant tissue culture technique to induce callus formation of Aloe vera on MS. Medium supplied with 10 mg/l NAA and 5 mg/l BA that exhibit the best results even with subculturing. As the method of [1] 1g. dru weight of callus induced from A. vera crown and in vivo crown were extracted then injected in HPLC using the standards of Ascorbic acid (vit. C), Salysilic acid and Nicotenic acid (vit. B5) to compare with the plant extracts. Results showed high potential of increasing some secondary products using the crown callus culture of A. vera as compared with in vivo crown, Ascorbic acid was 1.829 ?g/l in in vivo crown and increased to 3.905 ?g/l crown callus culture . Salysilic acid raised from 3.54 ?g/l in in vivo crown and reached to 25,487? g/l and the Nicotenic acid was 19.391 mg/l and decreased to 7.438 ?g/l.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1328
spellingShingle Baghdad Science Journal
Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture
مجلة بغداد للعلوم
title Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture
title_full Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture
title_fullStr Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture
title_full_unstemmed Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture
title_short Detection of Some Active compounds and Vitamins Increasing in Aloe vera Callus culture
title_sort detection of some active compounds and vitamins increasing in aloe vera callus culture
url http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1328
work_keys_str_mv AT baghdadsciencejournal detectionofsomeactivecompoundsandvitaminsincreasinginaloeveracallusculture