ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing
Designing dressings that mimic the composition, structure, and function of native skin is of great significance. Despite commercial decellularized extracellular matrix (dECM)-based wound dressings promoted skin regeneration process, reconstructing the fibrous microstructure of native dermis remains...
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Elsevier
2025-10-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006425007112 |
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| author | Jiutao Cao Shengchang Luo Wanling Huang Xiaochang Lu Ranjith Kumar Kankala Shibin Wang Peiyao Xu Aizheng Chen |
| author_facet | Jiutao Cao Shengchang Luo Wanling Huang Xiaochang Lu Ranjith Kumar Kankala Shibin Wang Peiyao Xu Aizheng Chen |
| author_sort | Jiutao Cao |
| collection | DOAJ |
| description | Designing dressings that mimic the composition, structure, and function of native skin is of great significance. Despite commercial decellularized extracellular matrix (dECM)-based wound dressings promoted skin regeneration process, reconstructing the fibrous microstructure of native dermis remains a significant challenge. In this work, a dECM-based composite nanofibrous membranes loaded with stem cell secretomes (SCS/dECMM) have been developed for accelerating wound repair, which comprehensively mimicked the dermal tissue in terms of composition, structure, and function. In detail, dermal dECM was prepared by eco-friendly supercritical carbon dioxide (SC-CO2) technology and then mixed with SCS to obtain SCS/dECMM by using electrospinning technology. The resulting nanofibrous membrane with 555.19 nm diameter that maintained SCS bioactivity while enabling sustained SCS release. SCS/dECMM significantly enhanced cell adhesion, proliferation, migration, and angiogenesis, thereby continuously promoting wound repair. In vivo wound healing results revealed that SCS/dECMM accelerated wound healing by promoting re-epithelialization, collagen deposition, and vascularization. SCS/dECMM offered a novel strategy for accelerating wound healing by replicating the composition, structure, and function of native skin. |
| format | Article |
| id | doaj-art-d7ace620a96b42ff9cd77ea932016e30 |
| institution | DOAJ |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-d7ace620a96b42ff9cd77ea932016e302025-08-20T02:47:28ZengElsevierMaterials Today Bio2590-00642025-10-013410214110.1016/j.mtbio.2025.102141ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healingJiutao Cao0Shengchang Luo1Wanling Huang2Xiaochang Lu3Ranjith Kumar Kankala4Shibin Wang5Peiyao Xu6Aizheng Chen7Fujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, ChinaFujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, ChinaMedical Sciences Division, Macau University of Science and Technology, Macau, 999078, ChinaFujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, ChinaFujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, ChinaFujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, ChinaFujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou, 510632, China; Corresponding author. Fujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, China.Fujian Provincial Key Laboratory of Biochemical Technology & Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen, 361021, China; Corresponding author.Designing dressings that mimic the composition, structure, and function of native skin is of great significance. Despite commercial decellularized extracellular matrix (dECM)-based wound dressings promoted skin regeneration process, reconstructing the fibrous microstructure of native dermis remains a significant challenge. In this work, a dECM-based composite nanofibrous membranes loaded with stem cell secretomes (SCS/dECMM) have been developed for accelerating wound repair, which comprehensively mimicked the dermal tissue in terms of composition, structure, and function. In detail, dermal dECM was prepared by eco-friendly supercritical carbon dioxide (SC-CO2) technology and then mixed with SCS to obtain SCS/dECMM by using electrospinning technology. The resulting nanofibrous membrane with 555.19 nm diameter that maintained SCS bioactivity while enabling sustained SCS release. SCS/dECMM significantly enhanced cell adhesion, proliferation, migration, and angiogenesis, thereby continuously promoting wound repair. In vivo wound healing results revealed that SCS/dECMM accelerated wound healing by promoting re-epithelialization, collagen deposition, and vascularization. SCS/dECMM offered a novel strategy for accelerating wound healing by replicating the composition, structure, and function of native skin.http://www.sciencedirect.com/science/article/pii/S2590006425007112dECMStem cell secretomeElectrospunNanofibrous membranesWound healing |
| spellingShingle | Jiutao Cao Shengchang Luo Wanling Huang Xiaochang Lu Ranjith Kumar Kankala Shibin Wang Peiyao Xu Aizheng Chen ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing Materials Today Bio dECM Stem cell secretome Electrospun Nanofibrous membranes Wound healing |
| title | ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing |
| title_full | ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing |
| title_fullStr | ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing |
| title_full_unstemmed | ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing |
| title_short | ECM-inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing |
| title_sort | ecm inspired stem cell secretome sustained releasing composite nanofibrous membranes for accelerated wound healing |
| topic | dECM Stem cell secretome Electrospun Nanofibrous membranes Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2590006425007112 |
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