Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide
This article explores sodium azide-induced mutagenesis in Triticum sinskajae cultivated in a test plot within the limits and suburbs of Ufa. The seeds of this diploid wheat were soaked in 0.1–0.6 mM sodium azide solutions in phosphate buffer (pH 3), the control group was not treated with any dosage....
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Kazan Federal University
2022-09-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2022-3-3.html |
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| author | A.R. Kuluev B.R. Kuluev A.V. Chemeris |
| author_facet | A.R. Kuluev B.R. Kuluev A.V. Chemeris |
| author_sort | A.R. Kuluev |
| collection | DOAJ |
| description | This article explores sodium azide-induced mutagenesis in Triticum sinskajae cultivated in a test plot within the limits and suburbs of Ufa. The seeds of this diploid wheat were soaked in 0.1–0.6 mM sodium azide solutions in phosphate buffer (pH 3), the control group was not treated with any dosage. The mutagenic effect was assessed by the morphometric changes in the obtained plants during their vegetation and fruiting. The genetic polymorphism of the mutant forms was identified using the ISSR analysis. With regard to the rates of seed germination and subsequent plant growth, the effective concentration of sodium azide for treating T. sinskajae was determined – 0.1 mM. The plants developed various morphological alternations in response to sodium azide: longer and shorter stems or spikes at low and high concentrations, respectively. No significant differences were found in the leaf length and 1000-grain weight between the mutant and wild types of plants. It was concluded that ISSR analysis is a promising diagnostic approach to assess the general mutagenic effect of chemical agents on the genome. Following on from the results of the survey, 78 mutant forms of T. sinskajae with short stems over a number of generations were selected. |
| format | Article |
| id | doaj-art-d7aa746b4b5a4e30be988fc8772df647 |
| institution | DOAJ |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-d7aa746b4b5a4e30be988fc8772df6472025-08-20T02:56:12ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2022-09-01164337839110.26907/2542-064X.2022.3.378-391Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azideA.R. Kuluev0B.R. Kuluev1A.V. Chemeris2Institute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa, 450054 RussiaInstitute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa, 450054 RussiaInstitute of Biochemistry and Genetics – Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, Ufa, 450054 RussiaThis article explores sodium azide-induced mutagenesis in Triticum sinskajae cultivated in a test plot within the limits and suburbs of Ufa. The seeds of this diploid wheat were soaked in 0.1–0.6 mM sodium azide solutions in phosphate buffer (pH 3), the control group was not treated with any dosage. The mutagenic effect was assessed by the morphometric changes in the obtained plants during their vegetation and fruiting. The genetic polymorphism of the mutant forms was identified using the ISSR analysis. With regard to the rates of seed germination and subsequent plant growth, the effective concentration of sodium azide for treating T. sinskajae was determined – 0.1 mM. The plants developed various morphological alternations in response to sodium azide: longer and shorter stems or spikes at low and high concentrations, respectively. No significant differences were found in the leaf length and 1000-grain weight between the mutant and wild types of plants. It was concluded that ISSR analysis is a promising diagnostic approach to assess the general mutagenic effect of chemical agents on the genome. Following on from the results of the survey, 78 mutant forms of T. sinskajae with short stems over a number of generations were selected.https://kpfu.ru/uz-eng-ns-2022-3-3.htmltriticum sinskajaemutationsodium azideissr analysisgenetic polymorphismdiploid wheatbreeding |
| spellingShingle | A.R. Kuluev B.R. Kuluev A.V. Chemeris Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide Учёные записки Казанского университета: Серия Естественные науки triticum sinskajae mutation sodium azide issr analysis genetic polymorphism diploid wheat breeding |
| title | Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide |
| title_full | Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide |
| title_fullStr | Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide |
| title_full_unstemmed | Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide |
| title_short | Chemical mutagenesis of Triticum sinskajae A. Filat. et Kurk. using sodium azide |
| title_sort | chemical mutagenesis of triticum sinskajae a filat et kurk using sodium azide |
| topic | triticum sinskajae mutation sodium azide issr analysis genetic polymorphism diploid wheat breeding |
| url | https://kpfu.ru/uz-eng-ns-2022-3-3.html |
| work_keys_str_mv | AT arkuluev chemicalmutagenesisoftriticumsinskajaeafilatetkurkusingsodiumazide AT brkuluev chemicalmutagenesisoftriticumsinskajaeafilatetkurkusingsodiumazide AT avchemeris chemicalmutagenesisoftriticumsinskajaeafilatetkurkusingsodiumazide |