Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau

Alpine meadows on the Qinghai–Tibetan Plateau are experiencing rapid degradation due to climate change and anthropogenic disturbances, leading to severe ecological consequences. In this study, we investigated the response of soil microbial communities and their metabolic functions across a degradati...

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Main Authors: Bingzhang Li, Quzhen Gesang, Yan Sun, Yuting Wang, Jibin Nan, Jun Xu
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/5/1142
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author Bingzhang Li
Quzhen Gesang
Yan Sun
Yuting Wang
Jibin Nan
Jun Xu
author_facet Bingzhang Li
Quzhen Gesang
Yan Sun
Yuting Wang
Jibin Nan
Jun Xu
author_sort Bingzhang Li
collection DOAJ
description Alpine meadows on the Qinghai–Tibetan Plateau are experiencing rapid degradation due to climate change and anthropogenic disturbances, leading to severe ecological consequences. In this study, we investigated the response of soil microbial communities and their metabolic functions across a degradation gradient using metagenomic sequencing and comprehensive soil physicochemical analysis in the city of Lhasa, China. Results showed that soil pH increased with degradation, while most nutrients, including different forms of nitrogen, phosphorus, and potassium, declined. pH, ammonium nitrogen, and organic matter were identified as key factors driving degradation dynamics. Microbial community composition shifted markedly, with distinct biomarker taxa emerging at different degradation levels. Network analysis revealed a progressive loss of microbial connectivity, with Actinobacteria dominance increasing in heavily degraded soils, while cross-phylum interactions weakened. Functional analysis of biogeochemical cycling genes showed that carbon, nitrogen, and phosphorus cycling were all disrupted by degradation, but each exhibited unique response patterns. These findings will extend our understanding of microbial-mediated soil processes under degradation and provide a scientific foundation for ecosystem management, conservation, and targeted restoration strategies in alpine meadows.
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spelling doaj-art-d7a0f1a1ba3040dda49deca78846b7c72025-08-20T01:56:41ZengMDPI AGMicroorganisms2076-26072025-05-01135114210.3390/microorganisms13051142Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan PlateauBingzhang Li0Quzhen Gesang1Yan Sun2Yuting Wang3Jibin Nan4Jun Xu5Tibet Academy of Forest Trees, Lasa 851400, ChinaTibet Academy of Forest Trees, Lasa 851400, ChinaTibet Academy of Forest Trees, Lasa 851400, ChinaTibet Academy of Forest Trees, Lasa 851400, ChinaTibet Academy of Forest Trees, Lasa 851400, ChinaTibet Academy of Forest Trees, Lasa 851400, ChinaAlpine meadows on the Qinghai–Tibetan Plateau are experiencing rapid degradation due to climate change and anthropogenic disturbances, leading to severe ecological consequences. In this study, we investigated the response of soil microbial communities and their metabolic functions across a degradation gradient using metagenomic sequencing and comprehensive soil physicochemical analysis in the city of Lhasa, China. Results showed that soil pH increased with degradation, while most nutrients, including different forms of nitrogen, phosphorus, and potassium, declined. pH, ammonium nitrogen, and organic matter were identified as key factors driving degradation dynamics. Microbial community composition shifted markedly, with distinct biomarker taxa emerging at different degradation levels. Network analysis revealed a progressive loss of microbial connectivity, with Actinobacteria dominance increasing in heavily degraded soils, while cross-phylum interactions weakened. Functional analysis of biogeochemical cycling genes showed that carbon, nitrogen, and phosphorus cycling were all disrupted by degradation, but each exhibited unique response patterns. These findings will extend our understanding of microbial-mediated soil processes under degradation and provide a scientific foundation for ecosystem management, conservation, and targeted restoration strategies in alpine meadows.https://www.mdpi.com/2076-2607/13/5/1142alpine meadow degradationsoil physicochemical propertiesmetagenomic sequencingmicroorganism communitybiogeochemical cycling
spellingShingle Bingzhang Li
Quzhen Gesang
Yan Sun
Yuting Wang
Jibin Nan
Jun Xu
Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau
Microorganisms
alpine meadow degradation
soil physicochemical properties
metagenomic sequencing
microorganism community
biogeochemical cycling
title Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau
title_full Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau
title_fullStr Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau
title_full_unstemmed Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau
title_short Soil Microbial Adaptation and Biogeochemical Feedback in Degraded Alpine Meadows of the Qinghai–Tibetan Plateau
title_sort soil microbial adaptation and biogeochemical feedback in degraded alpine meadows of the qinghai tibetan plateau
topic alpine meadow degradation
soil physicochemical properties
metagenomic sequencing
microorganism community
biogeochemical cycling
url https://www.mdpi.com/2076-2607/13/5/1142
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