Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents

Despite extensive research on the effects of sleep restriction on adolescent health, the field lacks experimental methods to study the health effects of mistimed sleep, which is also common among adolescents. This paper describes a novel 3-week experimental protocol that was designed to compare slee...

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Main Authors: Dean W. Beebe, Andrea L. Fidler, Laura McLaughlin, Sabrina Grove, Stephanie J. Crowley
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Clocks & Sleep
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Online Access:https://www.mdpi.com/2624-5175/7/1/4
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author Dean W. Beebe
Andrea L. Fidler
Laura McLaughlin
Sabrina Grove
Stephanie J. Crowley
author_facet Dean W. Beebe
Andrea L. Fidler
Laura McLaughlin
Sabrina Grove
Stephanie J. Crowley
author_sort Dean W. Beebe
collection DOAJ
description Despite extensive research on the effects of sleep restriction on adolescent health, the field lacks experimental methods to study the health effects of mistimed sleep, which is also common among adolescents. This paper describes a novel 3-week experimental protocol that was designed to compare sleep restriction, like what many adolescents experience on school nights, against sleep that meets the recommended duration but is timed to be relatively aligned or misaligned with their circadian phase. Healthy 14–18-year-olds, classified as early (“Lark”) and late (“Owl”) chronotypes, entered a six-night chronotype-aligned stabilization condition, followed by five nights of sleep restriction, a return to the stabilization schedule, and five nights of healthy sleep duration (HS). During HS, participants were randomly assigned to early-to-bed versus late-to-rise arms, intended to align with or misalign with their circadian phase. Actigraphy monitored sleep, and weekly dim-light melatonin onset (DLMO) assessed circadian phase. Analyses confirmed that the protocol met five key validation metrics related to differential attrition, sleep timing, circadian phase, and experimental induction of HS that is timed to be relatively aligned vs. misaligned with circadian phase. This protocol appears useful for future research into how misaligned sleep patterns, which occur regularly for many adolescents, may impact health.
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spelling doaj-art-d78e8a5815854004aa63644552bb647f2025-08-20T02:42:36ZengMDPI AGClocks & Sleep2624-51752025-01-0171410.3390/clockssleep7010004Feasibility of an At-Home Experimental Circadian Misalignment Induction for AdolescentsDean W. Beebe0Andrea L. Fidler1Laura McLaughlin2Sabrina Grove3Stephanie J. Crowley4Division of Behavioral Medicine and Clinical Psychology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USADepartment of Child & Adolescent Psychiatry and Behavioral Sciences, Children’s Hospital of Philadelphia, Philadelphia, PA 19104, USADivision of Behavioral Medicine and Clinical Psychology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USADivision of Behavioral Medicine and Clinical Psychology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH 45229, USADepartment of Psychiatry and Behavioral Sciences, Rush University System for Health, Chicago, IL 60612, USADespite extensive research on the effects of sleep restriction on adolescent health, the field lacks experimental methods to study the health effects of mistimed sleep, which is also common among adolescents. This paper describes a novel 3-week experimental protocol that was designed to compare sleep restriction, like what many adolescents experience on school nights, against sleep that meets the recommended duration but is timed to be relatively aligned or misaligned with their circadian phase. Healthy 14–18-year-olds, classified as early (“Lark”) and late (“Owl”) chronotypes, entered a six-night chronotype-aligned stabilization condition, followed by five nights of sleep restriction, a return to the stabilization schedule, and five nights of healthy sleep duration (HS). During HS, participants were randomly assigned to early-to-bed versus late-to-rise arms, intended to align with or misalign with their circadian phase. Actigraphy monitored sleep, and weekly dim-light melatonin onset (DLMO) assessed circadian phase. Analyses confirmed that the protocol met five key validation metrics related to differential attrition, sleep timing, circadian phase, and experimental induction of HS that is timed to be relatively aligned vs. misaligned with circadian phase. This protocol appears useful for future research into how misaligned sleep patterns, which occur regularly for many adolescents, may impact health.https://www.mdpi.com/2624-5175/7/1/4sleep manipulationcircadian phaseadolescence
spellingShingle Dean W. Beebe
Andrea L. Fidler
Laura McLaughlin
Sabrina Grove
Stephanie J. Crowley
Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents
Clocks & Sleep
sleep manipulation
circadian phase
adolescence
title Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents
title_full Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents
title_fullStr Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents
title_full_unstemmed Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents
title_short Feasibility of an At-Home Experimental Circadian Misalignment Induction for Adolescents
title_sort feasibility of an at home experimental circadian misalignment induction for adolescents
topic sleep manipulation
circadian phase
adolescence
url https://www.mdpi.com/2624-5175/7/1/4
work_keys_str_mv AT deanwbeebe feasibilityofanathomeexperimentalcircadianmisalignmentinductionforadolescents
AT andrealfidler feasibilityofanathomeexperimentalcircadianmisalignmentinductionforadolescents
AT lauramclaughlin feasibilityofanathomeexperimentalcircadianmisalignmentinductionforadolescents
AT sabrinagrove feasibilityofanathomeexperimentalcircadianmisalignmentinductionforadolescents
AT stephaniejcrowley feasibilityofanathomeexperimentalcircadianmisalignmentinductionforadolescents