Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1
Previously transgenic Kalanchoe pinnata plants producing an antimicrobial peptide cecropin P1 (CecP1) have been reported. Now we report biological testing K. pinnata extracts containing CecP1 as a candidate drug for treatment of wounds infected with Candida albicans. The drug constitutes the whole j...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/3940743 |
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| author | N. S. Zakharchenko A. S. Belous Y. K. Biryukova O. A. Medvedeva A. V. Belyakova G. A. Masgutova E. V. Trubnikova Y. I. Buryanov A. A. Lebedeva |
| author_facet | N. S. Zakharchenko A. S. Belous Y. K. Biryukova O. A. Medvedeva A. V. Belyakova G. A. Masgutova E. V. Trubnikova Y. I. Buryanov A. A. Lebedeva |
| author_sort | N. S. Zakharchenko |
| collection | DOAJ |
| description | Previously transgenic Kalanchoe pinnata plants producing an antimicrobial peptide cecropin P1 (CecP1) have been reported. Now we report biological testing K. pinnata extracts containing CecP1 as a candidate drug for treatment of wounds infected with Candida albicans. The drug constitutes the whole juice from K. pinnata leaves (not ethanol extract) sterilized with nanofiltration. A microbicide activity of CecP1 against an animal fungal pathogen in vivo was demonstrated for the first time. However, a favorable therapeutic effect of the transgenic K. pinnata extract was attributed to a synergism between the fungicide activity of CecP1 and wound healing (antiscar), revascularizing, and immunomodulating effect of natural biologically active components of K. pinnata. A commercial fungicide preparation clotrimazole eliminated C. albicans cells within infected wounds in rats with efficiency comparable to CecP1-enriched K. pinnata extract. But in contrast to K. pinnata extract, clotrimazole did not exhibit neither wound healing activity nor remodeling of the scar matrix. Taken together, our results allow assumption that CecP1-enriched K. pinnata extracts should be considered as a candidate drug for treatment of dermatomycoses, wounds infected with fungi, and bedsores. |
| format | Article |
| id | doaj-art-d787ba6191da4aaaadd0af1c83bc838a |
| institution | OA Journals |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-d787ba6191da4aaaadd0af1c83bc838a2025-08-20T02:20:58ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/39407433940743Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1N. S. Zakharchenko0A. S. Belous1Y. K. Biryukova2O. A. Medvedeva3A. V. Belyakova4G. A. Masgutova5E. V. Trubnikova6Y. I. Buryanov7A. A. Lebedeva8Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Сhemistry, Russian Academy of Sciences, Science Avenue 6, Pushchino, Moscow Region 142290, RussiaKursk State Medical University, K. Marksa St 3, Kursk 305041, RussiaEmanuel Institute of Biochemical Physics, Kosygina St 4, Moscow 119334, RussiaKursk State Medical University, K. Marksa St 3, Kursk 305041, RussiaChumakov Institute of Poliomyelitis and Viral Encephalitides, 27 km Kievskogo shosse, Moscow 142782, RussiaKazan Federal University, Kremlevskaya St 18, Kazan 420008, RussiaKursk State Medical University, K. Marksa St 3, Kursk 305041, RussiaBranch of Shemyakin-Ovchinnikov Institute of Bioorganic Сhemistry, Russian Academy of Sciences, Science Avenue 6, Pushchino, Moscow Region 142290, RussiaBranch of Shemyakin-Ovchinnikov Institute of Bioorganic Сhemistry, Russian Academy of Sciences, Science Avenue 6, Pushchino, Moscow Region 142290, RussiaPreviously transgenic Kalanchoe pinnata plants producing an antimicrobial peptide cecropin P1 (CecP1) have been reported. Now we report biological testing K. pinnata extracts containing CecP1 as a candidate drug for treatment of wounds infected with Candida albicans. The drug constitutes the whole juice from K. pinnata leaves (not ethanol extract) sterilized with nanofiltration. A microbicide activity of CecP1 against an animal fungal pathogen in vivo was demonstrated for the first time. However, a favorable therapeutic effect of the transgenic K. pinnata extract was attributed to a synergism between the fungicide activity of CecP1 and wound healing (antiscar), revascularizing, and immunomodulating effect of natural biologically active components of K. pinnata. A commercial fungicide preparation clotrimazole eliminated C. albicans cells within infected wounds in rats with efficiency comparable to CecP1-enriched K. pinnata extract. But in contrast to K. pinnata extract, clotrimazole did not exhibit neither wound healing activity nor remodeling of the scar matrix. Taken together, our results allow assumption that CecP1-enriched K. pinnata extracts should be considered as a candidate drug for treatment of dermatomycoses, wounds infected with fungi, and bedsores.http://dx.doi.org/10.1155/2017/3940743 |
| spellingShingle | N. S. Zakharchenko A. S. Belous Y. K. Biryukova O. A. Medvedeva A. V. Belyakova G. A. Masgutova E. V. Trubnikova Y. I. Buryanov A. A. Lebedeva Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1 Journal of Immunology Research |
| title | Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1 |
| title_full | Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1 |
| title_fullStr | Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1 |
| title_full_unstemmed | Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1 |
| title_short | Immunomodulating and Revascularizing Activity of Kalanchoe pinnata Synergize with Fungicide Activity of Biogenic Peptide Cecropin P1 |
| title_sort | immunomodulating and revascularizing activity of kalanchoe pinnata synergize with fungicide activity of biogenic peptide cecropin p1 |
| url | http://dx.doi.org/10.1155/2017/3940743 |
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