RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS
Background. Flow cytometry is one of the key technologies for acute lymphoblastic leukemia (ALL) diagnostics. Nevertheless lack of technological standards hampers implementation of immunophenotyping data in treatment protocols.Objective: development of harmonized guidelines for flow cytometric diagn...
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ABV-press
2018-05-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/278 |
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| author | I. A. Novikova T. Yu. Verzhbitskaya L. V. Movchan G. A. Tsaur М. V. Belevtsev A. M. Popov |
| author_facet | I. A. Novikova T. Yu. Verzhbitskaya L. V. Movchan G. A. Tsaur М. V. Belevtsev A. M. Popov |
| author_sort | I. A. Novikova |
| collection | DOAJ |
| description | Background. Flow cytometry is one of the key technologies for acute lymphoblastic leukemia (ALL) diagnostics. Nevertheless lack of technological standards hampers implementation of immunophenotyping data in treatment protocols.Objective: development of harmonized guidelines for flow cytometric diagnostics of childhood ALL. Materials and methods. Three reference laboratories of the immunophenotyping group “Moscow–Berlin” took part in the development and standardization of approaches to the cytometric determination of the minimal residual disease. The sequence of steps for staining with monoclonal antibodies, selection of reagents for immunophenotyping, flow cytometer adjustment algorithms were discussed.Results. We developed and implemented in multicenter setting the harmonized approach for immunophenotyping flow cytometric childhood ALL. Successful integration of this protocol in the multicenter group has shown good level of our approach reproducibility. Conclusion. These results will allow implementing diagnostic standards in stratification system of pediatric ALL treatment protocols of russianbelarusian multicenter group. |
| format | Article |
| id | doaj-art-d7836d8a4dc24a98af5d2ea567f1ef29 |
| institution | DOAJ |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2018-05-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-d7836d8a4dc24a98af5d2ea567f1ef292025-08-20T03:21:28ZrusABV-pressОнкогематология1818-83462413-40232018-05-01131738210.17650/1818-8346-2018-13-1-73-82262RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICSI. A. Novikova0T. Yu. Verzhbitskaya1L. V. Movchan2G. A. Tsaur3М. V. Belevtsev4A. M. Popov5D. Rogachev National Medical Research Center of Pediatric Hematology, Oncology and ImmunologyPediatric Oncology/Hematology Center, Regional Children Hospital № 1; Research Institute of Medical Cell TechnologiesBelarussian Research Center for Pediatric Oncology, Hematology and ImmunologyPediatric Oncology/Hematology Center, Regional Children Hospital № 1; Research Institute of Medical Cell TechnologiesBelarussian Research Center for Pediatric Oncology, Hematology and ImmunologyD. Rogachev National Medical Research Center of Pediatric Hematology, Oncology and ImmunologyBackground. Flow cytometry is one of the key technologies for acute lymphoblastic leukemia (ALL) diagnostics. Nevertheless lack of technological standards hampers implementation of immunophenotyping data in treatment protocols.Objective: development of harmonized guidelines for flow cytometric diagnostics of childhood ALL. Materials and methods. Three reference laboratories of the immunophenotyping group “Moscow–Berlin” took part in the development and standardization of approaches to the cytometric determination of the minimal residual disease. The sequence of steps for staining with monoclonal antibodies, selection of reagents for immunophenotyping, flow cytometer adjustment algorithms were discussed.Results. We developed and implemented in multicenter setting the harmonized approach for immunophenotyping flow cytometric childhood ALL. Successful integration of this protocol in the multicenter group has shown good level of our approach reproducibility. Conclusion. These results will allow implementing diagnostic standards in stratification system of pediatric ALL treatment protocols of russianbelarusian multicenter group.https://oncohematology.abvpress.ru/ongm/article/view/278acute lymphoblastic leukemiaflow cytometrystandardization |
| spellingShingle | I. A. Novikova T. Yu. Verzhbitskaya L. V. Movchan G. A. Tsaur М. V. Belevtsev A. M. Popov RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS Онкогематология acute lymphoblastic leukemia flow cytometry standardization |
| title | RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS |
| title_full | RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS |
| title_fullStr | RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS |
| title_full_unstemmed | RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS |
| title_short | RUSSIAN-BELARUSIAN MULTICENTER GROUP STANDARD GUIDELINES FOR CHILDHOOD ACUTE LYMPHOBLASTIC LEUKEMIA FLOW CYTOMETRIC DIAGNOSTICS |
| title_sort | russian belarusian multicenter group standard guidelines for childhood acute lymphoblastic leukemia flow cytometric diagnostics |
| topic | acute lymphoblastic leukemia flow cytometry standardization |
| url | https://oncohematology.abvpress.ru/ongm/article/view/278 |
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