The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish.
In vertebrates, skeletal myogenesis involves the sequential activation of myogenic factors to lead ultimately to the differentiation into slow and fast muscle fibers. How transcriptional co-regulators such as arginine methyltransferases PRMT4/CARM1 and PRMT5 control myogenesis in vivo remains poorly...
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025427&type=printable |
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| author | Julie Batut Carine Duboé Laurence Vandel |
| author_facet | Julie Batut Carine Duboé Laurence Vandel |
| author_sort | Julie Batut |
| collection | DOAJ |
| description | In vertebrates, skeletal myogenesis involves the sequential activation of myogenic factors to lead ultimately to the differentiation into slow and fast muscle fibers. How transcriptional co-regulators such as arginine methyltransferases PRMT4/CARM1 and PRMT5 control myogenesis in vivo remains poorly understood. Loss-of-function experiments using morpholinos against PRMT4/CARM1 and PRMT5 combined with in situ hybridization, quantitative polymerase chain reaction, as well as immunohistochemistry indicate a positive, but differential, role of these enzymes during myogenesis in vivo. While PRMT5 regulates myod, myf5 and myogenin expression and thereby slow and fast fiber formation, PRMT4/CARM1 regulates myogenin expression, fast fiber formation and does not affect slow fiber formation. However, our results show that PRMT4/CARM1 is required for proper slow myosin heavy chain localization. Altogether, our results reveal a combinatorial role of PRMT4/CARM1 and PRMT5 for proper myogenesis in zebrafish. |
| format | Article |
| id | doaj-art-d77efa4e30394c52b1a67b5ddd212393 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d77efa4e30394c52b1a67b5ddd2123932025-08-20T02:48:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01610e2542710.1371/journal.pone.0025427The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish.Julie BatutCarine DuboéLaurence VandelIn vertebrates, skeletal myogenesis involves the sequential activation of myogenic factors to lead ultimately to the differentiation into slow and fast muscle fibers. How transcriptional co-regulators such as arginine methyltransferases PRMT4/CARM1 and PRMT5 control myogenesis in vivo remains poorly understood. Loss-of-function experiments using morpholinos against PRMT4/CARM1 and PRMT5 combined with in situ hybridization, quantitative polymerase chain reaction, as well as immunohistochemistry indicate a positive, but differential, role of these enzymes during myogenesis in vivo. While PRMT5 regulates myod, myf5 and myogenin expression and thereby slow and fast fiber formation, PRMT4/CARM1 regulates myogenin expression, fast fiber formation and does not affect slow fiber formation. However, our results show that PRMT4/CARM1 is required for proper slow myosin heavy chain localization. Altogether, our results reveal a combinatorial role of PRMT4/CARM1 and PRMT5 for proper myogenesis in zebrafish.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025427&type=printable |
| spellingShingle | Julie Batut Carine Duboé Laurence Vandel The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish. PLoS ONE |
| title | The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish. |
| title_full | The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish. |
| title_fullStr | The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish. |
| title_full_unstemmed | The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish. |
| title_short | The methyltransferases PRMT4/CARM1 and PRMT5 control differentially myogenesis in zebrafish. |
| title_sort | methyltransferases prmt4 carm1 and prmt5 control differentially myogenesis in zebrafish |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0025427&type=printable |
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