Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults
ABSTRACT The concept of genome–microbiome interactions, in which the microenvironment determined by host genetic polymorphisms regulates the local microbiota, is important in the pathogenesis of human disease. In otolaryngology, the resident bacterial microbiota is reportedly altered in non-infectio...
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01698-24 |
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| author | Yasunobu Amari Masahiro Hosonuma Takuya Mizukami Junya Isobe Yuki Azetsu Eiji Funayama Yuki Maruyama Toshiaki Tsurui Kohei Tajima Aya Sasaki Yoshitaka Yamazaki Ryota Nakano Yutaka Sano Atsushi Ishida Tatsuya Nakanishi Seiji Mochizuki Yuri Yoshizawa Sumito Kumagai Sakiko Yasuhara Kakei Ryu Tatsunori Oguchi Atsuo Kuramasu Kiyoshi Yoshimura Takehiko Sambe Sei Kobayashi Naoki Uchida |
| author_facet | Yasunobu Amari Masahiro Hosonuma Takuya Mizukami Junya Isobe Yuki Azetsu Eiji Funayama Yuki Maruyama Toshiaki Tsurui Kohei Tajima Aya Sasaki Yoshitaka Yamazaki Ryota Nakano Yutaka Sano Atsushi Ishida Tatsuya Nakanishi Seiji Mochizuki Yuri Yoshizawa Sumito Kumagai Sakiko Yasuhara Kakei Ryu Tatsunori Oguchi Atsuo Kuramasu Kiyoshi Yoshimura Takehiko Sambe Sei Kobayashi Naoki Uchida |
| author_sort | Yasunobu Amari |
| collection | DOAJ |
| description | ABSTRACT The concept of genome–microbiome interactions, in which the microenvironment determined by host genetic polymorphisms regulates the local microbiota, is important in the pathogenesis of human disease. In otolaryngology, the resident bacterial microbiota is reportedly altered in non-infectious ear diseases, such as otitis media pearls and exudative otitis media. We hypothesized that a single-nucleotide polymorphism in the ATP-binding cassette sub-family C member 11 (ABCC11) gene, which determines earwax properties, regulates the ear canal microbiota. We analyzed ABCC11 gene polymorphisms and ear canal microbiota in healthy individuals to understand the relationship between genome–microbiome interactions in the ear canal. The study included 21 subjects who were divided into two groups: 538GA (9) and 538AA (12). Staphylococcus auricularis and Corynebacterium spp. were observed in the 538GA group, whereas Methylocella spp. was observed in the 538AA group. PICRUSt analysis revealed significant enrichment of certain pathways, such as superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation, chlorosalicylate degradation, mycothiol biosynthesis, and enterobactin biosynthesis in the GA group, whereas allantoin degradation IV (anaerobic), nitrifier denitrification, starch degradation III, L-valine degradation I, and nicotinate degradation I were significantly enriched in the AA group. The ABCC11 gene polymorphism regulates the composition of the ear canal microbiota and its metabolic pathways. This study revealed a genome–microbiome interaction within the resident microbiota of the external auditory canal that may help to elucidate the pathogenesis of ear diseases and develop novel therapies.IMPORTANCEThe ABCC11 gene polymorphism, which determines earwax characteristics, regulates the composition of the ear canal microbiota and its metabolic pathways. We determined the presence of genome–microbiome interactions in the resident microbiota of the ear canal. Future studies should focus on ABCC11 gene polymorphisms to elucidate the pathogenesis of ear diseases and develop therapeutic methods. |
| format | Article |
| id | doaj-art-d7721c51ec034e219c6fa7e680587c71 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
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| series | Microbiology Spectrum |
| spelling | doaj-art-d7721c51ec034e219c6fa7e680587c712025-02-04T14:03:40ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.01698-24Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adultsYasunobu Amari0Masahiro Hosonuma1Takuya Mizukami2Junya Isobe3Yuki Azetsu4Eiji Funayama5Yuki Maruyama6Toshiaki Tsurui7Kohei Tajima8Aya Sasaki9Yoshitaka Yamazaki10Ryota Nakano11Yutaka Sano12Atsushi Ishida13Tatsuya Nakanishi14Seiji Mochizuki15Yuri Yoshizawa16Sumito Kumagai17Sakiko Yasuhara18Kakei Ryu19Tatsunori Oguchi20Atsuo Kuramasu21Kiyoshi Yoshimura22Takehiko Sambe23Sei Kobayashi24Naoki Uchida25Department of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Hospital Pharmaceutics, School of Pharmacy, Showa University Graduate School of Pharmacy, Shinagawa, Tokyo, JapanPharmacological Research Center, Showa University, Shinagawa, Tokyo, JapanDepartment of Clinical Immuno Oncology, Clinical Research Institute for Clinical Pharmacology & Therapeutics, Showa University, Setagaya, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Clinical Immuno Oncology, Clinical Research Institute for Clinical Pharmacology & Therapeutics, Showa University, Setagaya, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanPharmacological Research Center, Showa University, Shinagawa, Tokyo, JapanDepartment of Physiology, Showa University Graduate School of Pharmacy, Shinagawa, Tokyo, JapanDepartment of Orthopaedic Surgery, Nihon University School of Medicine, Itabashi, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Clinical Immuno Oncology, Clinical Research Institute for Clinical Pharmacology & Therapeutics, Showa University, Setagaya, Tokyo, JapanDepartment of Clinical Immuno Oncology, Clinical Research Institute for Clinical Pharmacology & Therapeutics, Showa University, Setagaya, Tokyo, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanDepartment of Otolaryngology, Showa University Fujigaoka Hospital, Yokohama, Kanagawa, JapanDepartment of Pharmacology, Showa University Graduate School of Medicine, Shinagawa, Tokyo, JapanABSTRACT The concept of genome–microbiome interactions, in which the microenvironment determined by host genetic polymorphisms regulates the local microbiota, is important in the pathogenesis of human disease. In otolaryngology, the resident bacterial microbiota is reportedly altered in non-infectious ear diseases, such as otitis media pearls and exudative otitis media. We hypothesized that a single-nucleotide polymorphism in the ATP-binding cassette sub-family C member 11 (ABCC11) gene, which determines earwax properties, regulates the ear canal microbiota. We analyzed ABCC11 gene polymorphisms and ear canal microbiota in healthy individuals to understand the relationship between genome–microbiome interactions in the ear canal. The study included 21 subjects who were divided into two groups: 538GA (9) and 538AA (12). Staphylococcus auricularis and Corynebacterium spp. were observed in the 538GA group, whereas Methylocella spp. was observed in the 538AA group. PICRUSt analysis revealed significant enrichment of certain pathways, such as superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation, chlorosalicylate degradation, mycothiol biosynthesis, and enterobactin biosynthesis in the GA group, whereas allantoin degradation IV (anaerobic), nitrifier denitrification, starch degradation III, L-valine degradation I, and nicotinate degradation I were significantly enriched in the AA group. The ABCC11 gene polymorphism regulates the composition of the ear canal microbiota and its metabolic pathways. This study revealed a genome–microbiome interaction within the resident microbiota of the external auditory canal that may help to elucidate the pathogenesis of ear diseases and develop novel therapies.IMPORTANCEThe ABCC11 gene polymorphism, which determines earwax characteristics, regulates the composition of the ear canal microbiota and its metabolic pathways. We determined the presence of genome–microbiome interactions in the resident microbiota of the ear canal. Future studies should focus on ABCC11 gene polymorphisms to elucidate the pathogenesis of ear diseases and develop therapeutic methods.https://journals.asm.org/doi/10.1128/spectrum.01698-24ABCC11human microbiomegenome-microbiome interactionear canal |
| spellingShingle | Yasunobu Amari Masahiro Hosonuma Takuya Mizukami Junya Isobe Yuki Azetsu Eiji Funayama Yuki Maruyama Toshiaki Tsurui Kohei Tajima Aya Sasaki Yoshitaka Yamazaki Ryota Nakano Yutaka Sano Atsushi Ishida Tatsuya Nakanishi Seiji Mochizuki Yuri Yoshizawa Sumito Kumagai Sakiko Yasuhara Kakei Ryu Tatsunori Oguchi Atsuo Kuramasu Kiyoshi Yoshimura Takehiko Sambe Sei Kobayashi Naoki Uchida Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults Microbiology Spectrum ABCC11 human microbiome genome-microbiome interaction ear canal |
| title | Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults |
| title_full | Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults |
| title_fullStr | Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults |
| title_full_unstemmed | Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults |
| title_short | Association between the ABCC11 gene polymorphism-determined earwax properties and external auditory canal microbiota in healthy adults |
| title_sort | association between the abcc11 gene polymorphism determined earwax properties and external auditory canal microbiota in healthy adults |
| topic | ABCC11 human microbiome genome-microbiome interaction ear canal |
| url | https://journals.asm.org/doi/10.1128/spectrum.01698-24 |
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