Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis
Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time,...
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EDP Sciences
2024-01-01
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| Series: | Parasite |
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| Online Access: | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240102/parasite240102.html |
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| author | Cheng Yong Kang Zhang Yao Zhang Zhao Yu Cong Pei Kun Feng Ji Yu Zhang Ru Long Shao Rong Zhang Xi Wang Zhong Quan Cui Jing |
| author_facet | Cheng Yong Kang Zhang Yao Zhang Zhao Yu Cong Pei Kun Feng Ji Yu Zhang Ru Long Shao Rong Zhang Xi Wang Zhong Quan Cui Jing |
| author_sort | Cheng Yong Kang |
| collection | DOAJ |
| description | Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs. |
| format | Article |
| id | doaj-art-d77142e401864dd084a5d508fc562fc6 |
| institution | OA Journals |
| issn | 1776-1042 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
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| series | Parasite |
| spelling | doaj-art-d77142e401864dd084a5d508fc562fc62025-08-20T02:13:10ZengEDP SciencesParasite1776-10422024-01-01316510.1051/parasite/2024065parasite240102Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralisCheng Yong KangZhang YaoZhang Zhao YuCong Pei KunFeng Ji YuZhang RuLong Shao RongZhang XiWang Zhong QuanCui Jinghttps://orcid.org/0000-0001-8726-5498Glutamate dehydrogenase (GDH) plays an important role in the metabolism of organisms. Its high abundance in mitochondria in particular highlights its core role in cellular physiological processes. GDH catalyzes the mutual conversion between L-glutamic acid and α-ketoglutaric acids. At the same time, this transformation is accompanied by the oxidation-reduction of NAD(H) or NADP(H). This process not only helps to link amino acid metabolism with sugar metabolism, but also helps maintain the balance of intracellular pH and nitrogen homeostasis. In this study, a novel Trichinella spiralis glutamate dehydrogenase (TsGDH) was cloned, expressed and identified. The results revealed that TsGDH was expressed at various stages of development of the nematode T. spiralis, with higher expression levels in the adult worm stage, and was mainly localized in the cuticle, muscular layer, stichosome and female intrauterine embryos. After RNAi treatment, larval natural TsGDH enzyme activity was obviously reduced, and metabolism, molting, growth and reproduction were also significantly inhibited. The results indicate that TsGDH plays an important role in the development and survival of T. spiralis, and it may be a potential molecular target of anti-Trichinella vaccines and drugs.https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240102/parasite240102.htmltrichinella spiralisglutamate dehydrogenaserna interferencemetabolismmolting |
| spellingShingle | Cheng Yong Kang Zhang Yao Zhang Zhao Yu Cong Pei Kun Feng Ji Yu Zhang Ru Long Shao Rong Zhang Xi Wang Zhong Quan Cui Jing Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis Parasite trichinella spiralis glutamate dehydrogenase rna interference metabolism molting |
| title | Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis |
| title_full | Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis |
| title_fullStr | Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis |
| title_full_unstemmed | Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis |
| title_short | Biological characteristics and functions of a novel glutamate dehydrogenase from Trichinella spiralis |
| title_sort | biological characteristics and functions of a novel glutamate dehydrogenase from trichinella spiralis |
| topic | trichinella spiralis glutamate dehydrogenase rna interference metabolism molting |
| url | https://www.parasite-journal.org/articles/parasite/full_html/2024/01/parasite240102/parasite240102.html |
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