Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration
Abstract Background DMD gene has been implicated in the progression and development of several tumors, but its specific contribution to gastric cancer (GC) has not been fully elucidated. Methods We validated DMD gene expression levels in the TIMER2.0 database and human gastric cancer tissue microarr...
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BMC
2025-06-01
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| Series: | Cancer Cell International |
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| Online Access: | https://doi.org/10.1186/s12935-025-03829-4 |
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| author | Xiaodong Zou Tong Wu Tao Su Hui Xiao Chuyan Ni Lijuan Hu Wenchu Lin Weilin Chen Richard D Ye Jianjiao Lin Li Xiang |
| author_facet | Xiaodong Zou Tong Wu Tao Su Hui Xiao Chuyan Ni Lijuan Hu Wenchu Lin Weilin Chen Richard D Ye Jianjiao Lin Li Xiang |
| author_sort | Xiaodong Zou |
| collection | DOAJ |
| description | Abstract Background DMD gene has been implicated in the progression and development of several tumors, but its specific contribution to gastric cancer (GC) has not been fully elucidated. Methods We validated DMD gene expression levels in the TIMER2.0 database and human gastric cancer tissue microarrays and constructed gastric precancerous lesion mouse models and DMD gene knockout and overexpression cell lines to investigate the role of DMD gene in gastric cancer development. Results In this study, we found that DMD gene is significantly downregulated in GC cells and tissues. Mechanistic analysis revealed that DMD gene deletion increased intracellular calcium levels, disrupted mitochondrial homeostasis, and promoted a metabolic shift towards glycolysis, impacting GC cell proliferation and migration. Conclusions Taken together, our results shed light on the novel role of DMD gene in gastric cancer development and provide valuable insights into potential therapeutic strategies. |
| format | Article |
| id | doaj-art-d76ccd5246724df5bfafd27adb34ff25 |
| institution | OA Journals |
| issn | 1475-2867 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj-art-d76ccd5246724df5bfafd27adb34ff252025-08-20T02:31:09ZengBMCCancer Cell International1475-28672025-06-0125111510.1186/s12935-025-03829-4Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migrationXiaodong Zou0Tong Wu1Tao Su2Hui Xiao3Chuyan Ni4Lijuan Hu5Wenchu Lin6Weilin Chen7Richard D Ye8Jianjiao Lin9Li Xiang10Department of Gastroenterology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenKobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong KongDepartment of Gastroenterology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenDepartment of Pathology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenDepartment of Gastroenterology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenDepartment of Gastroenterology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenInstitute of Digestive Disease, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenMarshall Laboratory of Biomedical Engineering, Institute of Biological Therapy, Shenzhen University Medical School, Shenzhen UniversityKobilka Institute of Innovative Drug Discovery, School of Medicine, The Chinese University of Hong KongDepartment of Gastroenterology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenDepartment of Gastroenterology, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of ShenzhenAbstract Background DMD gene has been implicated in the progression and development of several tumors, but its specific contribution to gastric cancer (GC) has not been fully elucidated. Methods We validated DMD gene expression levels in the TIMER2.0 database and human gastric cancer tissue microarrays and constructed gastric precancerous lesion mouse models and DMD gene knockout and overexpression cell lines to investigate the role of DMD gene in gastric cancer development. Results In this study, we found that DMD gene is significantly downregulated in GC cells and tissues. Mechanistic analysis revealed that DMD gene deletion increased intracellular calcium levels, disrupted mitochondrial homeostasis, and promoted a metabolic shift towards glycolysis, impacting GC cell proliferation and migration. Conclusions Taken together, our results shed light on the novel role of DMD gene in gastric cancer development and provide valuable insights into potential therapeutic strategies.https://doi.org/10.1186/s12935-025-03829-4Gastric cancerDMD geneCalcium homeostasisMitochondrial dysfunctionGlycolysis |
| spellingShingle | Xiaodong Zou Tong Wu Tao Su Hui Xiao Chuyan Ni Lijuan Hu Wenchu Lin Weilin Chen Richard D Ye Jianjiao Lin Li Xiang Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration Cancer Cell International Gastric cancer DMD gene Calcium homeostasis Mitochondrial dysfunction Glycolysis |
| title | Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration |
| title_full | Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration |
| title_fullStr | Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration |
| title_full_unstemmed | Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration |
| title_short | Disruption of DMD gene leads to altered calcium homeostasis and metabolic shift impacting gastric Cancer cell proliferation and migration |
| title_sort | disruption of dmd gene leads to altered calcium homeostasis and metabolic shift impacting gastric cancer cell proliferation and migration |
| topic | Gastric cancer DMD gene Calcium homeostasis Mitochondrial dysfunction Glycolysis |
| url | https://doi.org/10.1186/s12935-025-03829-4 |
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