Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development
Wheat is one of the major food crops in the world. Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population. This study investigated the role of ABP7, a bHLH transcription factor from maize involved i...
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KeAi Communications Co., Ltd.
2023-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922001307 |
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| author | Zaid CHACHAR Siffat Ullah KHAN Xu-huan ZHANG Peng-fei LENG Na ZONG Jun ZHAO |
| author_facet | Zaid CHACHAR Siffat Ullah KHAN Xu-huan ZHANG Peng-fei LENG Na ZONG Jun ZHAO |
| author_sort | Zaid CHACHAR |
| collection | DOAJ |
| description | Wheat is one of the major food crops in the world. Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population. This study investigated the role of ABP7, a bHLH transcription factor from maize involved in kernel development, in regulating grain yield-related traits in transgenic wheat. Molecular characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene expression. At the same time, QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type (WT) plants. Phenotyping under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits (e.g., grain number per spike, grain weight per spike, thousand-grain weight, grain length, and grain width) and increased grain yield per plot, compared to WT plants, whereas line QB205 did not. In addition, total chlorophyll, chlorophyll a, chlorophyll b, and total soluble sugars were largely increased in the flag leaves of both HB123 and HB287 transgenic lines compared to the WT. These results strongly suggest that ABP7 positively regulates yield-related traits and plot grain yield in transgenic wheat. Consequently, ABP7 can be utilized in wheat breeding for grain yield improvement. |
| format | Article |
| id | doaj-art-d76604c3f14d43dea062d52960a5b989 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-d76604c3f14d43dea062d52960a5b9892025-08-20T03:57:39ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-02-0122238939910.1016/j.jia.2022.08.052Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel developmentZaid CHACHAR0Siffat Ullah KHAN1Xu-huan ZHANG2Peng-fei LENG3Na ZONG4Jun ZHAO5Faculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.ChinaFaculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.ChinaFaculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.ChinaFaculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.ChinaCorrespondence ZONG Na, Tel: +86-10-82106139; Faculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.ChinaCorrespondence ZHAO Jun, Tel: +86-10-82105320; Faculty of Maize Functional Genomics, National Key Facility for Crop Gene Resources and Genetic Improvement/Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100080, P.R.ChinaWheat is one of the major food crops in the world. Functional validation of the genes in increasing the grain yield of wheat by genetic engineering is essential for feeding the ever-growing global population. This study investigated the role of ABP7, a bHLH transcription factor from maize involved in kernel development, in regulating grain yield-related traits in transgenic wheat. Molecular characterization showed that transgenic lines HB123 and HB287 contained multicopy integration of ABP7 in the genome with higher transgene expression. At the same time, QB205 was a transgenic event of single copy insertion with no significant difference in ABP7 expression compared to wild-type (WT) plants. Phenotyping under field conditions showed that ABP7 over-expressing transgenic lines HB123 and HB287 exhibited improved grain yield-related traits (e.g., grain number per spike, grain weight per spike, thousand-grain weight, grain length, and grain width) and increased grain yield per plot, compared to WT plants, whereas line QB205 did not. In addition, total chlorophyll, chlorophyll a, chlorophyll b, and total soluble sugars were largely increased in the flag leaves of both HB123 and HB287 transgenic lines compared to the WT. These results strongly suggest that ABP7 positively regulates yield-related traits and plot grain yield in transgenic wheat. Consequently, ABP7 can be utilized in wheat breeding for grain yield improvement.http://www.sciencedirect.com/science/article/pii/S2095311922001307transgenic wheatABP7kernel developmentgrain weightgrain width |
| spellingShingle | Zaid CHACHAR Siffat Ullah KHAN Xu-huan ZHANG Peng-fei LENG Na ZONG Jun ZHAO Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development Journal of Integrative Agriculture transgenic wheat ABP7 kernel development grain weight grain width |
| title | Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development |
| title_full | Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development |
| title_fullStr | Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development |
| title_full_unstemmed | Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development |
| title_short | Characterization of transgenic wheat lines expressing maize ABP7 involved in kernel development |
| title_sort | characterization of transgenic wheat lines expressing maize abp7 involved in kernel development |
| topic | transgenic wheat ABP7 kernel development grain weight grain width |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922001307 |
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