Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment
Chlorella-based wastewater treatment presents a sustainable alternative to conventional methods. While previous studies highlighted Chlorella-bacteria synergy in wastewater remediation, bacterial effects on Chlorella biomass and biodiesel production remain unclear. This study investigates the impact...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Desalination and Water Treatment |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1944398625001663 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849734225354293248 |
|---|---|
| author | Xiuren Zhou Yuqing Li Pengming Yang Guifang Xu Hongsheng Wang |
| author_facet | Xiuren Zhou Yuqing Li Pengming Yang Guifang Xu Hongsheng Wang |
| author_sort | Xiuren Zhou |
| collection | DOAJ |
| description | Chlorella-based wastewater treatment presents a sustainable alternative to conventional methods. While previous studies highlighted Chlorella-bacteria synergy in wastewater remediation, bacterial effects on Chlorella biomass and biodiesel production remain unclear. This study investigates the impact of Bacillus licheniformis on Chlorella vulgaris growth, biomass yield, and biodiesel production. Results demonstrated that B. licheniformis significantly enhanced pollutant removal, achieving equal to or greater than 84.30 ± 2.61 % reduction in all wastewater contaminants, which was markedly superior to C. vulgaris alone. Furthermore, B. licheniformis treatment increased C. vulgaris biomass and biodiesel yield by 28.63 ± 2.88 % and 48.03 ± 3.42 %, respectively. The presence of B. licheniformis enriched the wastewater with bioavailable nutrients by producing hydrolytic enzymes such as N-protease (69.76 ± 8.60 U/mL) and α-amylase (25.78 ± 6.29 U/mL), as well as phytohormones, including auxins (554.98 ± 92.03 ng/mL) and gibberellins (30.08 ± 3.93 ng/mL). Additionally, the combined treatment stabilized pH fluctuations, creating an optimal environment for algal growth. These findings underscore the potential of integrating B. licheniformis with C. vulgaris to enhance wastewater treatment efficiency while simultaneously boosting biofuel production. This synergistic approach offers a strategy for sustainable wastewater management and bioenergy generation. |
| format | Article |
| id | doaj-art-d763f4b2764f4b3b83d011d62465490a |
| institution | DOAJ |
| issn | 1944-3986 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Desalination and Water Treatment |
| spelling | doaj-art-d763f4b2764f4b3b83d011d62465490a2025-08-20T03:07:50ZengElsevierDesalination and Water Treatment1944-39862025-04-0132210115010.1016/j.dwt.2025.101150Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatmentXiuren Zhou0Yuqing Li1Pengming Yang2Guifang Xu3Hongsheng Wang4School of Life Sciences, Henan Institute of Science and Technology, Huanlan Road, 98#, Xinxiang, Henan 453002, China; Corresponding author.School of Life Sciences, Henan Institute of Science and Technology, Huanlan Road, 98#, Xinxiang, Henan 453002, ChinaCollege of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Huanlan Road, 98#, Xinxiang, Henan 453002, ChinaSchool of Life Sciences, Henan Institute of Science and Technology, Huanlan Road, 98#, Xinxiang, Henan 453002, ChinaCollege of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Huanlan Road, 98#, Xinxiang, Henan 453002, ChinaChlorella-based wastewater treatment presents a sustainable alternative to conventional methods. While previous studies highlighted Chlorella-bacteria synergy in wastewater remediation, bacterial effects on Chlorella biomass and biodiesel production remain unclear. This study investigates the impact of Bacillus licheniformis on Chlorella vulgaris growth, biomass yield, and biodiesel production. Results demonstrated that B. licheniformis significantly enhanced pollutant removal, achieving equal to or greater than 84.30 ± 2.61 % reduction in all wastewater contaminants, which was markedly superior to C. vulgaris alone. Furthermore, B. licheniformis treatment increased C. vulgaris biomass and biodiesel yield by 28.63 ± 2.88 % and 48.03 ± 3.42 %, respectively. The presence of B. licheniformis enriched the wastewater with bioavailable nutrients by producing hydrolytic enzymes such as N-protease (69.76 ± 8.60 U/mL) and α-amylase (25.78 ± 6.29 U/mL), as well as phytohormones, including auxins (554.98 ± 92.03 ng/mL) and gibberellins (30.08 ± 3.93 ng/mL). Additionally, the combined treatment stabilized pH fluctuations, creating an optimal environment for algal growth. These findings underscore the potential of integrating B. licheniformis with C. vulgaris to enhance wastewater treatment efficiency while simultaneously boosting biofuel production. This synergistic approach offers a strategy for sustainable wastewater management and bioenergy generation.http://www.sciencedirect.com/science/article/pii/S1944398625001663Bacillus licheniformisBiomassBiodieselChlorella vulgarisHydrolytic enzymesMunicipal wastewater treatment |
| spellingShingle | Xiuren Zhou Yuqing Li Pengming Yang Guifang Xu Hongsheng Wang Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment Desalination and Water Treatment Bacillus licheniformis Biomass Biodiesel Chlorella vulgaris Hydrolytic enzymes Municipal wastewater treatment |
| title | Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment |
| title_full | Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment |
| title_fullStr | Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment |
| title_full_unstemmed | Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment |
| title_short | Bacillus licheniformis enhances Chlorella vulgaris’s biomass and biodiesel production in wastewater treatment |
| title_sort | bacillus licheniformis enhances chlorella vulgaris s biomass and biodiesel production in wastewater treatment |
| topic | Bacillus licheniformis Biomass Biodiesel Chlorella vulgaris Hydrolytic enzymes Municipal wastewater treatment |
| url | http://www.sciencedirect.com/science/article/pii/S1944398625001663 |
| work_keys_str_mv | AT xiurenzhou bacilluslicheniformisenhanceschlorellavulgarissbiomassandbiodieselproductioninwastewatertreatment AT yuqingli bacilluslicheniformisenhanceschlorellavulgarissbiomassandbiodieselproductioninwastewatertreatment AT pengmingyang bacilluslicheniformisenhanceschlorellavulgarissbiomassandbiodieselproductioninwastewatertreatment AT guifangxu bacilluslicheniformisenhanceschlorellavulgarissbiomassandbiodieselproductioninwastewatertreatment AT hongshengwang bacilluslicheniformisenhanceschlorellavulgarissbiomassandbiodieselproductioninwastewatertreatment |