Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination
This study investigates the inhibitory effects of Diaporthe sp. isolate EC010 extract on barnyardgrass (Echinochloa crus-galli) seed germination and growth. Application of sequential extraction techniques to Diaporthe sp. mycelium resulted in partial separation of the phytotoxic compounds. The ethyl...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-11-01
|
| Series: | Frontiers in Agronomy |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fagro.2024.1456168/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850129037710589952 |
|---|---|
| author | Naphat Somala Nutcha Manichart Chamroon Laosinwattana Pattharin Wichittrakarn Kaori Yoneyama Montinee Teerarak Nawasit Chotsaeng |
| author_facet | Naphat Somala Nutcha Manichart Chamroon Laosinwattana Pattharin Wichittrakarn Kaori Yoneyama Montinee Teerarak Nawasit Chotsaeng |
| author_sort | Naphat Somala |
| collection | DOAJ |
| description | This study investigates the inhibitory effects of Diaporthe sp. isolate EC010 extract on barnyardgrass (Echinochloa crus-galli) seed germination and growth. Application of sequential extraction techniques to Diaporthe sp. mycelium resulted in partial separation of the phytotoxic compounds. The ethyl acetate (EtOAc) fraction most greatly reduced seed germination (81.01%), root length (89.18%), and shoot length (84.74%) compared to the control. Chemical characterization using gas chromatography-mass spectrometry revealed major constituents of linoleic acid, butyl ester (9.69%), hexadecanoic acid (7.99%), and 14-pentadecenoic acid (7.86%). With regard to physiological and biochemical indexes, treated seeds exhibited lower imbibition, significantly decreased α-amylase (EC 3.2.1.1) activity (p<0.05), and increased accumulation of malondialdehyde (85.52%) and hydrogen peroxide (141.10%). Moreover, activity of the antioxidant enzymes superoxide dismutase (EC 1.15.1.1) and guaiacol peroxidase (EC 1.11.1.7) was upregulated (67.24 and 61.62%, respectively), while catalase (EC 1.11.1.6) activity was downregulated (-33.75%). The inference is that an imbalance in ROS levels combined with reduced antioxidant potential drives the gradual accumulation of oxidative damage in seed cells and consequent loss of seed viability. All told, these results confirm the Diaporthe extract to induce oxidative stress and inhibit antioxidant enzymes. This study clearly demonstrates the oxidative damage associated with Diaporthe allelochemicals. |
| format | Article |
| id | doaj-art-d749753ead5a4bd2897f49322e6b5e9c |
| institution | OA Journals |
| issn | 2673-3218 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Agronomy |
| spelling | doaj-art-d749753ead5a4bd2897f49322e6b5e9c2025-08-20T02:33:07ZengFrontiers Media S.A.Frontiers in Agronomy2673-32182024-11-01610.3389/fagro.2024.14561681456168Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germinationNaphat Somala0Nutcha Manichart1Chamroon Laosinwattana2Pattharin Wichittrakarn3Kaori Yoneyama4Montinee Teerarak5Nawasit Chotsaeng6School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, ThailandSchool of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, ThailandSchool of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, ThailandKing Mongkut Chaokhunthahan Hospital, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, ThailandDepartment of Biochemistry and Molecular Biology, Saitama University, Saitama, JapanSchool of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, ThailandSchool of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, ThailandThis study investigates the inhibitory effects of Diaporthe sp. isolate EC010 extract on barnyardgrass (Echinochloa crus-galli) seed germination and growth. Application of sequential extraction techniques to Diaporthe sp. mycelium resulted in partial separation of the phytotoxic compounds. The ethyl acetate (EtOAc) fraction most greatly reduced seed germination (81.01%), root length (89.18%), and shoot length (84.74%) compared to the control. Chemical characterization using gas chromatography-mass spectrometry revealed major constituents of linoleic acid, butyl ester (9.69%), hexadecanoic acid (7.99%), and 14-pentadecenoic acid (7.86%). With regard to physiological and biochemical indexes, treated seeds exhibited lower imbibition, significantly decreased α-amylase (EC 3.2.1.1) activity (p<0.05), and increased accumulation of malondialdehyde (85.52%) and hydrogen peroxide (141.10%). Moreover, activity of the antioxidant enzymes superoxide dismutase (EC 1.15.1.1) and guaiacol peroxidase (EC 1.11.1.7) was upregulated (67.24 and 61.62%, respectively), while catalase (EC 1.11.1.6) activity was downregulated (-33.75%). The inference is that an imbalance in ROS levels combined with reduced antioxidant potential drives the gradual accumulation of oxidative damage in seed cells and consequent loss of seed viability. All told, these results confirm the Diaporthe extract to induce oxidative stress and inhibit antioxidant enzymes. This study clearly demonstrates the oxidative damage associated with Diaporthe allelochemicals.https://www.frontiersin.org/articles/10.3389/fagro.2024.1456168/fullallelochemicalsbarnyardgrassDiaporthe sp.oxidative damagephytotoxicity |
| spellingShingle | Naphat Somala Nutcha Manichart Chamroon Laosinwattana Pattharin Wichittrakarn Kaori Yoneyama Montinee Teerarak Nawasit Chotsaeng Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination Frontiers in Agronomy allelochemicals barnyardgrass Diaporthe sp. oxidative damage phytotoxicity |
| title | Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination |
| title_full | Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination |
| title_fullStr | Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination |
| title_full_unstemmed | Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination |
| title_short | Oxidative damage in Echinochloa crus−galli seeds exposed to Diaporthe sp. (Diaporthales, Ascomycota) fungal extract during germination |
| title_sort | oxidative damage in echinochloa crus galli seeds exposed to diaporthe sp diaporthales ascomycota fungal extract during germination |
| topic | allelochemicals barnyardgrass Diaporthe sp. oxidative damage phytotoxicity |
| url | https://www.frontiersin.org/articles/10.3389/fagro.2024.1456168/full |
| work_keys_str_mv | AT naphatsomala oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination AT nutchamanichart oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination AT chamroonlaosinwattana oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination AT pattharinwichittrakarn oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination AT kaoriyoneyama oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination AT montineeteerarak oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination AT nawasitchotsaeng oxidativedamageinechinochloacrusgalliseedsexposedtodiaporthespdiaporthalesascomycotafungalextractduringgermination |