Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows

Genistin is an isoflavone of soybean, with estrogenic activity. This experiment was conducted to investigate its effect on reproductive performance, antioxidant capacity, and immunity in gestating sows. Seventy-two sows (Landrace × Yorkshire) were selected and randomly divided into two treatment gro...

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Main Authors: Yuchen Li, Bing Yu, Chunxue Liu, Shuangshuang Xia, Yuheng Luo, Ping Zheng, Guanglei Cong, Jie Yu, Junqiu Luo, Hui Yan, Jun He
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Veterinary Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2024.1489227/full
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author Yuchen Li
Bing Yu
Chunxue Liu
Shuangshuang Xia
Yuheng Luo
Ping Zheng
Guanglei Cong
Jie Yu
Junqiu Luo
Hui Yan
Jun He
author_facet Yuchen Li
Bing Yu
Chunxue Liu
Shuangshuang Xia
Yuheng Luo
Ping Zheng
Guanglei Cong
Jie Yu
Junqiu Luo
Hui Yan
Jun He
author_sort Yuchen Li
collection DOAJ
description Genistin is an isoflavone of soybean, with estrogenic activity. This experiment was conducted to investigate its effect on reproductive performance, antioxidant capacity, and immunity in gestating sows. Seventy-two sows (Landrace × Yorkshire) were selected and randomly divided into two treatment groups (n = 36) based on their backfat thickness, parity and fed with basal diet or supplementation of 150 mg/ kg genistin to the basal diet based on DMI for the entire gestation period. Results showed that dietary genistin supplementation significantly increased the average number of live born per litter (p < 0.05), and tended to increase the number of healthy piglets per litter (p = 0.058), but decreased the average weight of live born per litter (p < 0.05). Dietary genistin supplementation significantly decreased the number of mummified and stillbirths per litter (p < 0.05). Moreover, the average daily feed intake (ADFI) and total feed intake of the gestating sows were also increased in the genistin-supplemented group (p < 0.05). Genistin significantly increased the serum concentrations of catalase (CAT), immunoglobulin A (IgA), IgG, and IgM at 35 days of gestation (p < 0.05). The serum concentrations of interleukin-10 (IL-10) and interferon-γ (IFN-γ) were also increased upon genistin supplementation (p < 0.05). However, genistin supplementation tended to decrease the serum concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and leptin at 85 days of gestation (p = 0.081 and p = 0.096, respectively). Interestingly, genistin supplementation decreased the transcript abundance of interferon-γ (IFN-γ) and placental imprinting gene H19, but significantly increased the transcript abundance of insulin-like growth factor I (IGF-I) and amino acid transporters such as the sodium-coupled neutral amino acid transporter 2 (SNTA2) and SNAT4 in the placenta (p < 0.05). These results suggested that dietary genistin supplementation during gestation can improve the reproductive performance of sows, which was probably associated with improving of antioxidant capacity and immunity, as well as changes of transcript abundance of critical functional genes in the placenta.
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spelling doaj-art-d74200b9f11143e18179cb5115d6f2992024-11-21T12:14:28ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-11-011110.3389/fvets.2024.14892271489227Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sowsYuchen Li0Bing Yu1Chunxue Liu2Shuangshuang Xia3Yuheng Luo4Ping Zheng5Guanglei Cong6Jie Yu7Junqiu Luo8Hui Yan9Jun He10Institute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaAnyou Biotechnology Group Co., Ltd., Taicang, ChinaAnyou Biotechnology Group Co., Ltd., Taicang, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaAnyou Biotechnology Group Co., Ltd., Taicang, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaInstitute of Animal Nutrition Sichuan Agricultural University, Chengdu, ChinaGenistin is an isoflavone of soybean, with estrogenic activity. This experiment was conducted to investigate its effect on reproductive performance, antioxidant capacity, and immunity in gestating sows. Seventy-two sows (Landrace × Yorkshire) were selected and randomly divided into two treatment groups (n = 36) based on their backfat thickness, parity and fed with basal diet or supplementation of 150 mg/ kg genistin to the basal diet based on DMI for the entire gestation period. Results showed that dietary genistin supplementation significantly increased the average number of live born per litter (p < 0.05), and tended to increase the number of healthy piglets per litter (p = 0.058), but decreased the average weight of live born per litter (p < 0.05). Dietary genistin supplementation significantly decreased the number of mummified and stillbirths per litter (p < 0.05). Moreover, the average daily feed intake (ADFI) and total feed intake of the gestating sows were also increased in the genistin-supplemented group (p < 0.05). Genistin significantly increased the serum concentrations of catalase (CAT), immunoglobulin A (IgA), IgG, and IgM at 35 days of gestation (p < 0.05). The serum concentrations of interleukin-10 (IL-10) and interferon-γ (IFN-γ) were also increased upon genistin supplementation (p < 0.05). However, genistin supplementation tended to decrease the serum concentrations of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and leptin at 85 days of gestation (p = 0.081 and p = 0.096, respectively). Interestingly, genistin supplementation decreased the transcript abundance of interferon-γ (IFN-γ) and placental imprinting gene H19, but significantly increased the transcript abundance of insulin-like growth factor I (IGF-I) and amino acid transporters such as the sodium-coupled neutral amino acid transporter 2 (SNTA2) and SNAT4 in the placenta (p < 0.05). These results suggested that dietary genistin supplementation during gestation can improve the reproductive performance of sows, which was probably associated with improving of antioxidant capacity and immunity, as well as changes of transcript abundance of critical functional genes in the placenta.https://www.frontiersin.org/articles/10.3389/fvets.2024.1489227/fullgenistingestation sowreproductive performanceserum biochemistryplacental gene
spellingShingle Yuchen Li
Bing Yu
Chunxue Liu
Shuangshuang Xia
Yuheng Luo
Ping Zheng
Guanglei Cong
Jie Yu
Junqiu Luo
Hui Yan
Jun He
Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows
Frontiers in Veterinary Science
genistin
gestation sow
reproductive performance
serum biochemistry
placental gene
title Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows
title_full Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows
title_fullStr Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows
title_full_unstemmed Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows
title_short Effects of dietary genistin supplementation on reproductive performance, immunity and antioxidative capacity in gestating sows
title_sort effects of dietary genistin supplementation on reproductive performance immunity and antioxidative capacity in gestating sows
topic genistin
gestation sow
reproductive performance
serum biochemistry
placental gene
url https://www.frontiersin.org/articles/10.3389/fvets.2024.1489227/full
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