HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers
IntroductionThis study investigates the impact of HTLV-2 infection on small RNA (sRNA) expression profiles, building on previous findings related to HTLV-1.MethodsUsing Illumina Massive Parallel Sequencing, we analyzed sRNA profiles from asymptomatic HTLV-1 and HTLV-2 infected individuals and health...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2025.1547712/full |
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| author | Lorena Abreu Fernandes Victor Ângelo Folgosi Rodrigo Pessôa Tatiane Assone Jefferson Russo Victor Jorge Casseb Augusto César Penalva de Oliveira Youko Nukui Alberto José da Silva Duarte Sabri Saeed Sanabani Sabri Saeed Sanabani |
| author_facet | Lorena Abreu Fernandes Victor Ângelo Folgosi Rodrigo Pessôa Tatiane Assone Jefferson Russo Victor Jorge Casseb Augusto César Penalva de Oliveira Youko Nukui Alberto José da Silva Duarte Sabri Saeed Sanabani Sabri Saeed Sanabani |
| author_sort | Lorena Abreu Fernandes |
| collection | DOAJ |
| description | IntroductionThis study investigates the impact of HTLV-2 infection on small RNA (sRNA) expression profiles, building on previous findings related to HTLV-1.MethodsUsing Illumina Massive Parallel Sequencing, we analyzed sRNA profiles from asymptomatic HTLV-1 and HTLV-2 infected individuals and healthy controls.ResultsOur results reveal significant differential expression of 331 known and 441 novel sRNAs among the groups, including miRNAs, piRNAs, and tRNAs. Notably, distinct clusters of sRNA expression patterns were identified, with specific miRNAs showing significant upregulation or downregulation in HTLV-1 and HTLV-2 infections. Gene Ontology analysis indicated significant involvement of target genes in transcription regulation and RNA-binding processes, while KEGG pathway analysis highlighted enrichment in cancer-related pathways and signaling cascades such as FoxO, Ras, and MAPK. Network analysis identified key miRNAs, such as hsa-miR-20b-5p and hsa-let-7e-5p, as central regulators with extensive interactions, suggesting their potential role in the pathogenesis and immune response of HTLV infections.DiscussionThese findings provide a foundation for future research into the molecular mechanisms of HTLV infections and the development of targeted therapies. The identified sRNAs, especially important miRNAs such as hsa-miR-20b-5p and hsa-let-7e-5p, could serve as potential biomarkers for disease progression or as therapeutic targets to modulate the immune response and disrupt viral pathogenesis. This opens up new avenues for precision medicine in HTLV-associated diseases. |
| format | Article |
| id | doaj-art-d73ae4bbbf4d47bd93254ff25a99f394 |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-d73ae4bbbf4d47bd93254ff25a99f3942025-08-20T03:01:01ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-02-011210.3389/fmed.2025.15477121547712HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriersLorena Abreu Fernandes0Victor Ângelo Folgosi1Rodrigo Pessôa2Tatiane Assone3Jefferson Russo Victor4Jorge Casseb5Augusto César Penalva de Oliveira6Youko Nukui7Alberto José da Silva Duarte8Sabri Saeed Sanabani9Sabri Saeed Sanabani10Post-Graduation Program in Translational Medicine, Department of Medicine, Federal University of São Paulo, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of São Paulo Medical School, São Paulo, BrazilPost-Graduation Program in Translational Medicine, Department of Medicine, Federal University of São Paulo, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of São Paulo Medical School, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of São Paulo Medical School, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of São Paulo Medical School, São Paulo, BrazilDepartment of Neurology, Institute of Infectology Emílio Ribas (IIER), São Paulo, BrazilDepartment of Hematology, Faculty of Medicine, University of São Paulo, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of São Paulo Medical School, São Paulo, BrazilLaboratory of Medical Investigation LIM-56, Division of Dermatology, University of São Paulo Medical School, São Paulo, BrazilLaboratory of Medical Investigation 03 (LIM03), Clinics Hospital, Faculty of Medicine, University of São Paulo, São Paulo, BrazilIntroductionThis study investigates the impact of HTLV-2 infection on small RNA (sRNA) expression profiles, building on previous findings related to HTLV-1.MethodsUsing Illumina Massive Parallel Sequencing, we analyzed sRNA profiles from asymptomatic HTLV-1 and HTLV-2 infected individuals and healthy controls.ResultsOur results reveal significant differential expression of 331 known and 441 novel sRNAs among the groups, including miRNAs, piRNAs, and tRNAs. Notably, distinct clusters of sRNA expression patterns were identified, with specific miRNAs showing significant upregulation or downregulation in HTLV-1 and HTLV-2 infections. Gene Ontology analysis indicated significant involvement of target genes in transcription regulation and RNA-binding processes, while KEGG pathway analysis highlighted enrichment in cancer-related pathways and signaling cascades such as FoxO, Ras, and MAPK. Network analysis identified key miRNAs, such as hsa-miR-20b-5p and hsa-let-7e-5p, as central regulators with extensive interactions, suggesting their potential role in the pathogenesis and immune response of HTLV infections.DiscussionThese findings provide a foundation for future research into the molecular mechanisms of HTLV infections and the development of targeted therapies. The identified sRNAs, especially important miRNAs such as hsa-miR-20b-5p and hsa-let-7e-5p, could serve as potential biomarkers for disease progression or as therapeutic targets to modulate the immune response and disrupt viral pathogenesis. This opens up new avenues for precision medicine in HTLV-associated diseases.https://www.frontiersin.org/articles/10.3389/fmed.2025.1547712/fullHTLV-1HTLV-2sRNA expressionGene OntologyKEGG pathwaysmiRNAs |
| spellingShingle | Lorena Abreu Fernandes Victor Ângelo Folgosi Rodrigo Pessôa Tatiane Assone Jefferson Russo Victor Jorge Casseb Augusto César Penalva de Oliveira Youko Nukui Alberto José da Silva Duarte Sabri Saeed Sanabani Sabri Saeed Sanabani HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers Frontiers in Medicine HTLV-1 HTLV-2 sRNA expression Gene Ontology KEGG pathways miRNAs |
| title | HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers |
| title_full | HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers |
| title_fullStr | HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers |
| title_full_unstemmed | HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers |
| title_short | HTLV-1 and HTLV-2 infections significantly alter small RNA expression in asymptomatic carriers |
| title_sort | htlv 1 and htlv 2 infections significantly alter small rna expression in asymptomatic carriers |
| topic | HTLV-1 HTLV-2 sRNA expression Gene Ontology KEGG pathways miRNAs |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2025.1547712/full |
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