Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms
Phosphorus deficiency critically constrains crop growth. Soil microbial diversity, which is crucial for maintaining terrestrial ecosystem integrity, plays a key role in promoting soil P cycling. Therefore, it is imperative to understand the survival strategies of microorganisms under P-limited condi...
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MDPI AG
2024-11-01
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| author | Junjie Hu Yanhao Lian Hui Guo Zongzhen Li Haifang Pang Mengjiao Zhang Yongzhe Ren Tongbao Lin Zhiqiang Wang |
| author_facet | Junjie Hu Yanhao Lian Hui Guo Zongzhen Li Haifang Pang Mengjiao Zhang Yongzhe Ren Tongbao Lin Zhiqiang Wang |
| author_sort | Junjie Hu |
| collection | DOAJ |
| description | Phosphorus deficiency critically constrains crop growth. Soil microbial diversity, which is crucial for maintaining terrestrial ecosystem integrity, plays a key role in promoting soil P cycling. Therefore, it is imperative to understand the survival strategies of microorganisms under P-limited conditions and explore their roles in community regulation. We initiated a comprehensive, long-term, in situ wheat field experiment to measure soil physicochemical properties, focusing on the different forms of soil inorganic P. Subsequently, 16S rRNA and ITS marker sequencing was employed to study changes in soil microbial abundance and community structure and predict functional alterations. The results showed that soil water and P deficiencies significantly affected wheat growth and development, soil physicochemical properties, and microbial diversity and function. Prolonged P deficiency lowered soil pH, significantly increasing phosphatase content (58%) under W1 (normal irrigation) conditions. Divalent calcium phosphate decreased significantly under W0 (lack of irrigation) and W1 conditions, and the most stable ten-valent calcium phosphate began to transform under W0 conditions. Soil microbial diversity increased (e.g., <i>Proteobacteria</i> and <i>Vicinamibacterales</i>) and enhanced the transport capacity of bacteria. P deficiency affected the coexistence networks between bacteria and fungi, and SEM (structural equation modeling) analysis revealed a stronger correlation in bacteria (r<sup>2</sup> = 0.234) than in fungi (r<sup>2</sup> = 0.172). In soils deprived of P for 7 years, the soil P content and forms were coupled with microbial changes. Microorganisms exhibited community and functional changes in response to low-phosphorus soil, concurrently influencing soil P status. This study enhances our understanding of rhizospheric processes in soil P cycling under microbial feedback, particularly the impact of microbial interactions on changes in soil P forms under P-limited conditions. |
| format | Article |
| id | doaj-art-d71791cc43f541c0a2aebda926f8cdda |
| institution | OA Journals |
| issn | 2077-0472 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-d71791cc43f541c0a2aebda926f8cdda2025-08-20T02:26:51ZengMDPI AGAgriculture2077-04722024-11-011411202210.3390/agriculture14112022Response of Long-Term Water and Phosphorus of Wheat to Soil MicroorganismsJunjie Hu0Yanhao Lian1Hui Guo2Zongzhen Li3Haifang Pang4Mengjiao Zhang5Yongzhe Ren6Tongbao Lin7Zhiqiang Wang8College of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaCollege of Agriculture, Henan Agricultural University, Zhengzhou 450002, ChinaPhosphorus deficiency critically constrains crop growth. Soil microbial diversity, which is crucial for maintaining terrestrial ecosystem integrity, plays a key role in promoting soil P cycling. Therefore, it is imperative to understand the survival strategies of microorganisms under P-limited conditions and explore their roles in community regulation. We initiated a comprehensive, long-term, in situ wheat field experiment to measure soil physicochemical properties, focusing on the different forms of soil inorganic P. Subsequently, 16S rRNA and ITS marker sequencing was employed to study changes in soil microbial abundance and community structure and predict functional alterations. The results showed that soil water and P deficiencies significantly affected wheat growth and development, soil physicochemical properties, and microbial diversity and function. Prolonged P deficiency lowered soil pH, significantly increasing phosphatase content (58%) under W1 (normal irrigation) conditions. Divalent calcium phosphate decreased significantly under W0 (lack of irrigation) and W1 conditions, and the most stable ten-valent calcium phosphate began to transform under W0 conditions. Soil microbial diversity increased (e.g., <i>Proteobacteria</i> and <i>Vicinamibacterales</i>) and enhanced the transport capacity of bacteria. P deficiency affected the coexistence networks between bacteria and fungi, and SEM (structural equation modeling) analysis revealed a stronger correlation in bacteria (r<sup>2</sup> = 0.234) than in fungi (r<sup>2</sup> = 0.172). In soils deprived of P for 7 years, the soil P content and forms were coupled with microbial changes. Microorganisms exhibited community and functional changes in response to low-phosphorus soil, concurrently influencing soil P status. This study enhances our understanding of rhizospheric processes in soil P cycling under microbial feedback, particularly the impact of microbial interactions on changes in soil P forms under P-limited conditions.https://www.mdpi.com/2077-0472/14/11/2022wheatphosphorus deficiencymicrobial diversitymicrobial functionmicrobial interaction |
| spellingShingle | Junjie Hu Yanhao Lian Hui Guo Zongzhen Li Haifang Pang Mengjiao Zhang Yongzhe Ren Tongbao Lin Zhiqiang Wang Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms Agriculture wheat phosphorus deficiency microbial diversity microbial function microbial interaction |
| title | Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms |
| title_full | Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms |
| title_fullStr | Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms |
| title_full_unstemmed | Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms |
| title_short | Response of Long-Term Water and Phosphorus of Wheat to Soil Microorganisms |
| title_sort | response of long term water and phosphorus of wheat to soil microorganisms |
| topic | wheat phosphorus deficiency microbial diversity microbial function microbial interaction |
| url | https://www.mdpi.com/2077-0472/14/11/2022 |
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