A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation
This study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The iSperm system integrates a tablet, optical module,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-06-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/6/394 |
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| author | Shu-Sheng Lin Chang-Yu Chen Cheng-Ming Lin Tsun-Chao Chiang Yu-Siang Tang Chang-Ching Yeh Wei-Fan Hsu Andrew M. Wo |
| author_facet | Shu-Sheng Lin Chang-Yu Chen Cheng-Ming Lin Tsun-Chao Chiang Yu-Siang Tang Chang-Ching Yeh Wei-Fan Hsu Andrew M. Wo |
| author_sort | Shu-Sheng Lin |
| collection | DOAJ |
| description | This study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The iSperm system integrates a tablet, optical module, heater, and real-time image analysis app to deliver automated measurements of sperm concentration, motility, and progressive motility in under one minute. Precision and user variability tests demonstrated high concordance with CASA and the hemocytometer, with minimal differences between trained and untrained users. A method comparison using 77 farm-collected samples confirmed agreement through Passing–Bablok regression and Bland–Altman analysis. ROC curve analyses further validated diagnostic accuracy for all parameters, with AUC values exceeding 0.95. The iSperm platform offers a reliable, user-friendly, and field-deployable solution for on-site semen quality assessment, improving decision-making in swine artificial insemination. |
| format | Article |
| id | doaj-art-d7173ded4b2a43c88bfbee74c4ed7f0a |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-d7173ded4b2a43c88bfbee74c4ed7f0a2025-08-20T03:27:13ZengMDPI AGBiosensors2079-63742025-06-0115639410.3390/bios15060394A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen EvaluationShu-Sheng Lin0Chang-Yu Chen1Cheng-Ming Lin2Tsun-Chao Chiang3Yu-Siang Tang4Chang-Ching Yeh5Wei-Fan Hsu6Andrew M. Wo7Institute of Applied Mechanics, National Taiwan University, Taipei 106319, TaiwanAidmics Biotechnology, Taipei 10647, TaiwanAidmics Biotechnology, Taipei 10647, TaiwanAidmics Biotechnology, Taipei 10647, TaiwanAidmics Biotechnology, Taipei 10647, TaiwanAidmics Biotechnology, Taipei 10647, TaiwanInstitute of Applied Mechanics, National Taiwan University, Taipei 106319, TaiwanInstitute of Applied Mechanics, National Taiwan University, Taipei 106319, TaiwanThis study presents a mobile-based sperm analysis system featuring a user-friendly, droplet-loaded microfluidic chip that enables non-specialist users to perform the rapid and accurate quantitative evaluation of boar semen directly on the farm. The iSperm system integrates a tablet, optical module, heater, and real-time image analysis app to deliver automated measurements of sperm concentration, motility, and progressive motility in under one minute. Precision and user variability tests demonstrated high concordance with CASA and the hemocytometer, with minimal differences between trained and untrained users. A method comparison using 77 farm-collected samples confirmed agreement through Passing–Bablok regression and Bland–Altman analysis. ROC curve analyses further validated diagnostic accuracy for all parameters, with AUC values exceeding 0.95. The iSperm platform offers a reliable, user-friendly, and field-deployable solution for on-site semen quality assessment, improving decision-making in swine artificial insemination.https://www.mdpi.com/2079-6374/15/6/394portable sperm analysis systemboar semen analysison-farm point-of-care testingmicrofluidic chipimage processinguser variability assessment |
| spellingShingle | Shu-Sheng Lin Chang-Yu Chen Cheng-Ming Lin Tsun-Chao Chiang Yu-Siang Tang Chang-Ching Yeh Wei-Fan Hsu Andrew M. Wo A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation Biosensors portable sperm analysis system boar semen analysis on-farm point-of-care testing microfluidic chip image processing user variability assessment |
| title | A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation |
| title_full | A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation |
| title_fullStr | A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation |
| title_full_unstemmed | A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation |
| title_short | A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation |
| title_sort | mobile sperm analyzer with user friendly microfluidic chips for rapid on farm semen evaluation |
| topic | portable sperm analysis system boar semen analysis on-farm point-of-care testing microfluidic chip image processing user variability assessment |
| url | https://www.mdpi.com/2079-6374/15/6/394 |
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