A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference

Bayesian phylogenetic analysis is essential for elucidating evolutionary relationships among organisms. Traditional methods often rely on fixed models and manual parameter settings, which can limit accuracy and efficiency. This protocol presents an integrated workflow that leverages GUIDANCE2 for ri...

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Main Authors: Jinxing Wang, Fangmin Chen, Xu Xiao, Xinyao Yang, Wanting Xia
Format: Article
Language:English
Published: Bio-protocol LLC 2025-04-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5276&type=0
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author Jinxing Wang
Fangmin Chen
Xu Xiao
Xinyao Yang
Wanting Xia
author_facet Jinxing Wang
Fangmin Chen
Xu Xiao
Xinyao Yang
Wanting Xia
author_sort Jinxing Wang
collection DOAJ
description Bayesian phylogenetic analysis is essential for elucidating evolutionary relationships among organisms. Traditional methods often rely on fixed models and manual parameter settings, which can limit accuracy and efficiency. This protocol presents an integrated workflow that leverages GUIDANCE2 for rigorous sequence alignment, ProtTest and MrModeltest for robust model selection, and MrBayes for phylogenetic tree estimation through Bayesian inference. By automating key steps and providing detailed command-line instructions, this protocol enhances the reliability and reproducibility of phylogenetic studies.
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spelling doaj-art-d707514dcaac485391e98b2c6213f5f72025-08-20T03:48:46ZengBio-protocol LLCBio-Protocol2331-83252025-04-0115810.21769/BioProtoc.5276A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic InferenceJinxing Wang0Fangmin Chen1Xu Xiao2Xinyao Yang3Wanting Xia4Liaoning Province Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Bioengineering, Shenyang University, Liaoning, ChinaLiaoning Province Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Bioengineering, Shenyang University, Liaoning, ChinaKey Laboratory of Ecological Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang, Liaoning, ChinaSchool of Information Engineering, Wenzhou Business College, Wenzhou, Zhejiang, ChinaLiaoning Province Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Bioengineering, Shenyang University, Liaoning, ChinaKey Laboratory of Ecological Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang, Liaoning, ChinaLiaoning Province Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Bioengineering, Shenyang University, Liaoning, ChinaKey Laboratory of Ecological Restoration of Regional Contaminated Environment, Ministry of Education, Shenyang University, Shenyang, Liaoning, ChinaBayesian phylogenetic analysis is essential for elucidating evolutionary relationships among organisms. Traditional methods often rely on fixed models and manual parameter settings, which can limit accuracy and efficiency. This protocol presents an integrated workflow that leverages GUIDANCE2 for rigorous sequence alignment, ProtTest and MrModeltest for robust model selection, and MrBayes for phylogenetic tree estimation through Bayesian inference. By automating key steps and providing detailed command-line instructions, this protocol enhances the reliability and reproducibility of phylogenetic studies.https://bio-protocol.org/en/bpdetail?id=5276&type=0
spellingShingle Jinxing Wang
Fangmin Chen
Xu Xiao
Xinyao Yang
Wanting Xia
A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference
Bio-Protocol
title A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference
title_full A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference
title_fullStr A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference
title_full_unstemmed A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference
title_short A Comprehensive Protocol for Bayesian Phylogenetic Analysis Using MrBayes: From Sequence Alignment to Model Selection and Phylogenetic Inference
title_sort comprehensive protocol for bayesian phylogenetic analysis using mrbayes from sequence alignment to model selection and phylogenetic inference
url https://bio-protocol.org/en/bpdetail?id=5276&type=0
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