Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets
<i>Cordyceps militaris</i> extract (CME) contains many bioactive compounds, mainly cordycepin (CPN). This study aimed to investigate the possible mechanisms underlying the amelioration of LPS-induced acute liver injury in piglets by CME or CPN supplementation using multi-omics analysis....
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2024-10-01
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| author | Ding Tan Endian Li Shijie Xiong Yue Sun Wenbo Cheng Yong Su Yang Lu |
| author_facet | Ding Tan Endian Li Shijie Xiong Yue Sun Wenbo Cheng Yong Su Yang Lu |
| author_sort | Ding Tan |
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| description | <i>Cordyceps militaris</i> extract (CME) contains many bioactive compounds, mainly cordycepin (CPN). This study aimed to investigate the possible mechanisms underlying the amelioration of LPS-induced acute liver injury in piglets by CME or CPN supplementation using multi-omics analysis. Twenty-four weaned piglets were randomly distributed into 4 groups (<i>n</i> = 6): the control and LPS groups were fed basal diets; the CPN + LPS (CPN-LPS) and CME + LPS (CME-LPS) groups were fed the basal diets supplemented with CME or CPN. The results showed that CPN or CME supplementation significantly decreased the C-reactive protein level (<i>p</i> < 0.05) and improved liver tissue pathology to prevent acute liver injury after LPS treatment. Compared with LPS, the transcriptomic analysis indicated that CPN supplementation significantly downregulated cell adhesion molecules, while CME supplementation significantly downregulated inflammatory mediator regulation of TRP channels, complement and coagulation cascades and cytokine-cytokine receptor interaction. The metabolomic results showed that CPN or CME supplementation significantly reduced disease biomarker of bicyclo-prostaglandin E2, and increased levels of deoxyinosine and 3-hydroxyanthranilic acid (<i>p</i> < 0.05). The combined transcriptome and metabolome helped identify two metabolites PC 34:2 and PC 36:0, which may be associated with the restoration of liver cell morphology. In conclusion, CPN and CME could attenuate LPS-induced acute liver injury by regulating immune-related genes and metabolites. This study elucidates the potential protective mechanism of CPN or CME supplementation against acute liver injury. |
| format | Article |
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| issn | 2076-2615 |
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| spelling | doaj-art-d6fcb334bd654693adc58adfe835beed2025-08-20T01:47:41ZengMDPI AGAnimals2076-26152024-10-011419287310.3390/ani14192873Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in PigletsDing Tan0Endian Li1Shijie Xiong2Yue Sun3Wenbo Cheng4Yong Su5Yang Lu6Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaLaboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China<i>Cordyceps militaris</i> extract (CME) contains many bioactive compounds, mainly cordycepin (CPN). This study aimed to investigate the possible mechanisms underlying the amelioration of LPS-induced acute liver injury in piglets by CME or CPN supplementation using multi-omics analysis. Twenty-four weaned piglets were randomly distributed into 4 groups (<i>n</i> = 6): the control and LPS groups were fed basal diets; the CPN + LPS (CPN-LPS) and CME + LPS (CME-LPS) groups were fed the basal diets supplemented with CME or CPN. The results showed that CPN or CME supplementation significantly decreased the C-reactive protein level (<i>p</i> < 0.05) and improved liver tissue pathology to prevent acute liver injury after LPS treatment. Compared with LPS, the transcriptomic analysis indicated that CPN supplementation significantly downregulated cell adhesion molecules, while CME supplementation significantly downregulated inflammatory mediator regulation of TRP channels, complement and coagulation cascades and cytokine-cytokine receptor interaction. The metabolomic results showed that CPN or CME supplementation significantly reduced disease biomarker of bicyclo-prostaglandin E2, and increased levels of deoxyinosine and 3-hydroxyanthranilic acid (<i>p</i> < 0.05). The combined transcriptome and metabolome helped identify two metabolites PC 34:2 and PC 36:0, which may be associated with the restoration of liver cell morphology. In conclusion, CPN and CME could attenuate LPS-induced acute liver injury by regulating immune-related genes and metabolites. This study elucidates the potential protective mechanism of CPN or CME supplementation against acute liver injury.https://www.mdpi.com/2076-2615/14/19/2873piglets<i>Cordyceps militaris</i> extractcordycepinliver injurymetabolomics and transcriptomics |
| spellingShingle | Ding Tan Endian Li Shijie Xiong Yue Sun Wenbo Cheng Yong Su Yang Lu Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets Animals piglets <i>Cordyceps militaris</i> extract cordycepin liver injury metabolomics and transcriptomics |
| title | Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets |
| title_full | Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets |
| title_fullStr | Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets |
| title_full_unstemmed | Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets |
| title_short | Transcriptomic and Metabolomic Analyses Reveal the Attenuating Role of Cordycepin and <i>Cordyceps militaris</i> Extract on Acute Liver Injury Induced by LPS in Piglets |
| title_sort | transcriptomic and metabolomic analyses reveal the attenuating role of cordycepin and i cordyceps militaris i extract on acute liver injury induced by lps in piglets |
| topic | piglets <i>Cordyceps militaris</i> extract cordycepin liver injury metabolomics and transcriptomics |
| url | https://www.mdpi.com/2076-2615/14/19/2873 |
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