Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS
Abstract This study presents an investigation of the chemical composition of Aloe vera leaf tissue with a focus on the spatial distribution of compounds. The composition was studied using two mass spectrometry imaging techniques: silver-109 nanoparticles assisted laser desorption/ionization mass spe...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-88144-8 |
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| author | Sumi Krupa Tomasz Ruman Wiktoria Szuberla Joanna Nizioł |
| author_facet | Sumi Krupa Tomasz Ruman Wiktoria Szuberla Joanna Nizioł |
| author_sort | Sumi Krupa |
| collection | DOAJ |
| description | Abstract This study presents an investigation of the chemical composition of Aloe vera leaf tissue with a focus on the spatial distribution of compounds. The composition was studied using two mass spectrometry imaging techniques: silver-109 nanoparticles assisted laser desorption/ionization mass spectrometry imaging (109AgNPs-LDI-MSI) and laser ablation-remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging (LARAPPI/CI-MSI) and the identification was aided by ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS) analysis. The results showed an abundance of phenolic compounds with antioxidant, antimicrobial, and anti-inflammatory properties, making it a beneficial food additive and food packaging material. Analysis of the results of mass spectrometry imaging provided information about the potential changes in metabolic pathway expression in different regions of the leaf. |
| format | Article |
| id | doaj-art-d6f7d1bd72ea40c48ae8dc42bcf6ef0b |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d6f7d1bd72ea40c48ae8dc42bcf6ef0b2025-02-02T12:18:11ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-025-88144-8Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMSSumi Krupa0Tomasz Ruman1Wiktoria Szuberla2Joanna Nizioł3Doctoral School, Rzeszów University of TechnologyDepartment of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of TechnologyDepartment of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of TechnologyDepartment of Polymers and Biopolymers, Faculty of Chemistry, Rzeszów University of TechnologyAbstract This study presents an investigation of the chemical composition of Aloe vera leaf tissue with a focus on the spatial distribution of compounds. The composition was studied using two mass spectrometry imaging techniques: silver-109 nanoparticles assisted laser desorption/ionization mass spectrometry imaging (109AgNPs-LDI-MSI) and laser ablation-remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging (LARAPPI/CI-MSI) and the identification was aided by ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS) analysis. The results showed an abundance of phenolic compounds with antioxidant, antimicrobial, and anti-inflammatory properties, making it a beneficial food additive and food packaging material. Analysis of the results of mass spectrometry imaging provided information about the potential changes in metabolic pathway expression in different regions of the leaf.https://doi.org/10.1038/s41598-025-88144-8Aloe veraMass spectrometry imagingLaser ablationMetabolomicsPlant tissueAmbient ionization |
| spellingShingle | Sumi Krupa Tomasz Ruman Wiktoria Szuberla Joanna Nizioł Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS Scientific Reports Aloe vera Mass spectrometry imaging Laser ablation Metabolomics Plant tissue Ambient ionization |
| title | Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS |
| title_full | Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS |
| title_fullStr | Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS |
| title_full_unstemmed | Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS |
| title_short | Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS |
| title_sort | analysis of the spatial distribution of metabolites in aloe vera leaves by mass spectrometry imaging and uhplc uhrms |
| topic | Aloe vera Mass spectrometry imaging Laser ablation Metabolomics Plant tissue Ambient ionization |
| url | https://doi.org/10.1038/s41598-025-88144-8 |
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