AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor
Purpose: We have previously demonstrated that the AgNOR count in proliferating cells is a predictor of tumor recurrence in superficial bladder tumor (J. Urol. 162 (1999), 63–68). In the present study, we evaluate the type of AgNOR associated with cell cycles as a prognostic factor in invasive bladde...
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| Format: | Article |
| Language: | English |
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Wiley
2001-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2001/689480 |
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| _version_ | 1832565063479721984 |
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| author | Mitsuro Tomobe Toru Shimazui Katsunori Uchida Hideyuki Akaza |
| author_facet | Mitsuro Tomobe Toru Shimazui Katsunori Uchida Hideyuki Akaza |
| author_sort | Mitsuro Tomobe |
| collection | DOAJ |
| description | Purpose: We have previously demonstrated that the AgNOR count in proliferating cells is a predictor of tumor recurrence in superficial bladder tumor (J. Urol. 162 (1999), 63–68). In the present study, we evaluate the type of AgNOR associated with cell cycles as a prognostic factor in invasive bladder tumor using a double staining technique employing both AgNOR and MIB-1 labelling. Materials and methods: Forty-four paraffin sections of invasive bladder tumors were stained simultaneously with AgNOR and MIB-1. The number of AgNORs in proliferating (MIB-1 positive) or resting (MIB-1 negative) cells were counted from a total of 100 nuclei. Correlations between MIB-1 associated AgNOR count and clinicopathological parameters were statistically analyzed. Results: The AgNOR count in proliferating cells (proliferating NOR) was significantly higher than that in resting cells (resting NOR) (p < 0.01). The resting NOR in tumors with distant metastases was significantly higher than that in tumors without metastases (p < 0.05). Patients with a low resting NOR tumor had a better prognosis than those with a high resting NOR tumor, whereas the proliferating NOR was not associated with survival. Survival analysis revealed that the resting NOR was the most powerful prognostic marker in patients with invasive bladder tumor (p < 0.05). Conclusions: Resting NOR had a predictive value in the prognosis of patients with invasive bladder tumor. Keywords: Transitional cell carcinoma, invasive, resting cell, AgNORs, MIB-1 |
| format | Article |
| id | doaj-art-d6f251b1d028421e98c3131621aee8ef |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2001-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-d6f251b1d028421e98c3131621aee8ef2025-02-03T01:09:38ZengWileyAnalytical Cellular Pathology0921-89121878-36512001-01-0122419319910.1155/2001/689480AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder TumorMitsuro Tomobe0Toru Shimazui1Katsunori Uchida2Hideyuki Akaza3Department of Urology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba Ibaraki, JapanDepartment of Urology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba Ibaraki, JapanDepartment of Urology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba Ibaraki, JapanDepartment of Urology, Institute of Clinical Medicine, University of Tsukuba, Tsukuba Ibaraki, JapanPurpose: We have previously demonstrated that the AgNOR count in proliferating cells is a predictor of tumor recurrence in superficial bladder tumor (J. Urol. 162 (1999), 63–68). In the present study, we evaluate the type of AgNOR associated with cell cycles as a prognostic factor in invasive bladder tumor using a double staining technique employing both AgNOR and MIB-1 labelling. Materials and methods: Forty-four paraffin sections of invasive bladder tumors were stained simultaneously with AgNOR and MIB-1. The number of AgNORs in proliferating (MIB-1 positive) or resting (MIB-1 negative) cells were counted from a total of 100 nuclei. Correlations between MIB-1 associated AgNOR count and clinicopathological parameters were statistically analyzed. Results: The AgNOR count in proliferating cells (proliferating NOR) was significantly higher than that in resting cells (resting NOR) (p < 0.01). The resting NOR in tumors with distant metastases was significantly higher than that in tumors without metastases (p < 0.05). Patients with a low resting NOR tumor had a better prognosis than those with a high resting NOR tumor, whereas the proliferating NOR was not associated with survival. Survival analysis revealed that the resting NOR was the most powerful prognostic marker in patients with invasive bladder tumor (p < 0.05). Conclusions: Resting NOR had a predictive value in the prognosis of patients with invasive bladder tumor. Keywords: Transitional cell carcinoma, invasive, resting cell, AgNORs, MIB-1http://dx.doi.org/10.1155/2001/689480 |
| spellingShingle | Mitsuro Tomobe Toru Shimazui Katsunori Uchida Hideyuki Akaza AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor Analytical Cellular Pathology |
| title | AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor |
| title_full | AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor |
| title_fullStr | AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor |
| title_full_unstemmed | AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor |
| title_short | AgNOR Count in Resting Cells (Resting NOR) Is a New Prognostic Marker in Invasive Bladder Tumor |
| title_sort | agnor count in resting cells resting nor is a new prognostic marker in invasive bladder tumor |
| url | http://dx.doi.org/10.1155/2001/689480 |
| work_keys_str_mv | AT mitsurotomobe agnorcountinrestingcellsrestingnorisanewprognosticmarkerininvasivebladdertumor AT torushimazui agnorcountinrestingcellsrestingnorisanewprognosticmarkerininvasivebladdertumor AT katsunoriuchida agnorcountinrestingcellsrestingnorisanewprognosticmarkerininvasivebladdertumor AT hideyukiakaza agnorcountinrestingcellsrestingnorisanewprognosticmarkerininvasivebladdertumor |