Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia
Highlight Research • The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples. • AMOVA revealed that the diversity among individuals in the populations were higher compared to those within indivi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Fisheries and Marine Universitas Airlangga
2023-01-01
|
| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/36686 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849683504988684288 |
|---|---|
| author | Hatika Hairani Rizka Amelia Ipanna Enggar Susetya Arida Susilowati Yuntha Bimantara Tadashi Kajita Mohammad Basyuni |
| author_facet | Hatika Hairani Rizka Amelia Ipanna Enggar Susetya Arida Susilowati Yuntha Bimantara Tadashi Kajita Mohammad Basyuni |
| author_sort | Hatika Hairani |
| collection | DOAJ |
| description | Highlight Research
• The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples.
• AMOVA revealed that the diversity among individuals in the populations were higher compared to those within individuals.
• DNA barcode in mangroves is useful in developing mangrove conservation and restoration initiatives
Abstract
Fish and shrimp are among the species that are vulnerable to high level of disturbance in mangrove ecosystem. This study aimed to investigate the implementation of 16S rRNA gene for fish and shrimp barcoding in mangrove ecosystems in North Sumatra and Aceh, Indonesia. 50 samples fresh fish and shrimp were collected from five mangrove ecosystems in North Sumatra, namely Belawan, Lubuk Kertang, Percut Sei Tuan, and Pulau Sembilan and Langsa of Aceh for DNA extraction process. The research was divided into four activities: sample collection, DNA isolation, PCR amplification, and data analysis. DNA was extracted using kit extraction (kit Reliaprep gDNA Tissue Miniprep System) and with PCR amplification. Genetic parameters were analyzed using Gen Alex 6.51 software and the relationship between sites was examined using the MVSP 3.22. The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, according to the findings, with a heterozygosity of 0.926, with the exception of Langsa samples, which were only identified in subclass A1. Genetic relationship between sites revealed that all samples clustered into two branches and were randomly dispersed within each site. This work confirmed the 16S rRNA gene worked for fish and shrimp barcoding in mangrove ecosystems, North Sumatra and Aceh, Indonesia and our findings are expected to be useful in developing mangrove conservation and restoration initiatives. |
| format | Article |
| id | doaj-art-d6e911f485604e40a3b2d4c820ea8d67 |
| institution | DOAJ |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-d6e911f485604e40a3b2d4c820ea8d672025-08-20T03:23:51ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592023-01-0115115216110.20473/jipk.v15i1.3668634727Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, IndonesiaHatika Hairani0Rizka Amelia1Ipanna Enggar Susetya2Arida Susilowati3Yuntha Bimantara4Tadashi Kajita5Mohammad Basyuni6https://orcid.org/0000-0003-1120-8571Department of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155. IndonesiaDepartment of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155. IndonesiaDepartment of Aquatic Resources Management, Faculty of Agriculture, Universitas Sumatera Utara, Medan, 20155. IndonesiaCenter of Excellence for Mangrove, Universitas Sumatra Utara, Medan, 20155. IndonesiaDepartment of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155. IndonesiaIriomote Station, Tropical Biosphere Research Center, University of the Ryukyus, Taketomi, Okinawa. JapanDepartment of Forestry, Faculty of Forestry, Universitas Sumatera Utara, Medan, 20155. IndonesiaHighlight Research • The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, with a heterozygosity of 0.926 and the exception of Langsa samples. • AMOVA revealed that the diversity among individuals in the populations were higher compared to those within individuals. • DNA barcode in mangroves is useful in developing mangrove conservation and restoration initiatives Abstract Fish and shrimp are among the species that are vulnerable to high level of disturbance in mangrove ecosystem. This study aimed to investigate the implementation of 16S rRNA gene for fish and shrimp barcoding in mangrove ecosystems in North Sumatra and Aceh, Indonesia. 50 samples fresh fish and shrimp were collected from five mangrove ecosystems in North Sumatra, namely Belawan, Lubuk Kertang, Percut Sei Tuan, and Pulau Sembilan and Langsa of Aceh for DNA extraction process. The research was divided into four activities: sample collection, DNA isolation, PCR amplification, and data analysis. DNA was extracted using kit extraction (kit Reliaprep gDNA Tissue Miniprep System) and with PCR amplification. Genetic parameters were analyzed using Gen Alex 6.51 software and the relationship between sites was examined using the MVSP 3.22. The genetic diversity of fish and shrimp from mangrove habitats was relatively substantial, according to the findings, with a heterozygosity of 0.926, with the exception of Langsa samples, which were only identified in subclass A1. Genetic relationship between sites revealed that all samples clustered into two branches and were randomly dispersed within each site. This work confirmed the 16S rRNA gene worked for fish and shrimp barcoding in mangrove ecosystems, North Sumatra and Aceh, Indonesia and our findings are expected to be useful in developing mangrove conservation and restoration initiatives.https://e-journal.unair.ac.id/JIPK/article/view/36686conservationdna barcodinggenetic diversitygenetic relationshippcr amplificationrestoration |
| spellingShingle | Hatika Hairani Rizka Amelia Ipanna Enggar Susetya Arida Susilowati Yuntha Bimantara Tadashi Kajita Mohammad Basyuni Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia Jurnal Ilmiah Perikanan dan Kelautan conservation dna barcoding genetic diversity genetic relationship pcr amplification restoration |
| title | Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia |
| title_full | Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia |
| title_fullStr | Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia |
| title_full_unstemmed | Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia |
| title_short | Implementation of 16S rRNA Gene for Fish and Shrimp Barcoding in Mangrove Ecosystems in North Sumatra and Aceh, Indonesia |
| title_sort | implementation of 16s rrna gene for fish and shrimp barcoding in mangrove ecosystems in north sumatra and aceh indonesia |
| topic | conservation dna barcoding genetic diversity genetic relationship pcr amplification restoration |
| url | https://e-journal.unair.ac.id/JIPK/article/view/36686 |
| work_keys_str_mv | AT hatikahairani implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia AT rizkaamelia implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia AT ipannaenggarsusetya implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia AT aridasusilowati implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia AT yunthabimantara implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia AT tadashikajita implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia AT mohammadbasyuni implementationof16srrnageneforfishandshrimpbarcodinginmangroveecosystemsinnorthsumatraandacehindonesia |