Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study
Introduction. For the treatment of overactive bladder syndrome (OAB), injection of botulinum toxin A (BoNT-A) has been shown to be effective. However, there is a need for a less invasive method for administering BoNT-A, which could significantly expand the treatment options for OAB.Objective. To ass...
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Ministry of Health of Russian Federation, Rostov State Medical University, State Budget Educational Institute of Higher Professional Education
2024-07-01
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| Series: | Вестник урологии |
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| Online Access: | https://www.urovest.ru/jour/article/view/885 |
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| author | S. V. Poroyskiy D. V. Perlin O. G. Srussovskaya N. A. Goncharov A. A. Kuznetsov E. A. Morozov |
| author_facet | S. V. Poroyskiy D. V. Perlin O. G. Srussovskaya N. A. Goncharov A. A. Kuznetsov E. A. Morozov |
| author_sort | S. V. Poroyskiy |
| collection | DOAJ |
| description | Introduction. For the treatment of overactive bladder syndrome (OAB), injection of botulinum toxin A (BoNT-A) has been shown to be effective. However, there is a need for a less invasive method for administering BoNT-A, which could significantly expand the treatment options for OAB.Objective. To assess the impact of tizol on the absorption of BoNT-A by the bladder mucosa and compare it to the individual absorption of BoNT-A.Materials & Methods. Dialysis through the mucous membrane of the сalf bladder was used as an experimental model to study changes in bioavailability of BoNT-A complexed with tisol (BoNT-A + T) and pure BoNT-A solution during in vitro experiment. After dialysis, the BoNT-A concentration in both samples was determined using a spectrophotometer. Dialysis curves were plotted according to the data obtained. Kruvchinsky equilibrium dialysis method was used to determine botulinum toxin A bioavailability. The UV spectrophotometry method was used to determine the concentration of BoNT-A in the acceptor medium by reaction of BoNT-A with Benedict's reagent.Results. It was established that the maximum concentration of BoNT-A diffused into the acceptor medium from the blend of the test substance with tizol after nine hours. The area under the curve for dialysis of BoNT-A + T exceeds the area under the curve of pure BoNT-A by almost 20%, suggesting an improvement in the drug's bioavailability when blended with tizol.Conclusion. Based on our experiment, it was found out that the BoNT-A + T has greater bioavailability than a solution of pure BoNT-A. However, the diffusion rate of the component mixture is sufficiently low. |
| format | Article |
| id | doaj-art-d6d82a8053fc49e49d312ca711ca6f59 |
| institution | Kabale University |
| issn | 2308-6424 |
| language | Russian |
| publishDate | 2024-07-01 |
| publisher | Ministry of Health of Russian Federation, Rostov State Medical University, State Budget Educational Institute of Higher Professional Education |
| record_format | Article |
| series | Вестник урологии |
| spelling | doaj-art-d6d82a8053fc49e49d312ca711ca6f592025-08-20T03:43:33ZrusMinistry of Health of Russian Federation, Rostov State Medical University, State Budget Educational Institute of Higher Professional EducationВестник урологии2308-64242024-07-01123798710.21886/2308-6424-2024-12-3-79-87533Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental studyS. V. Poroyskiy0D. V. Perlin1O. G. Srussovskaya2N. A. Goncharov3A. A. Kuznetsov4E. A. Morozov5Volgograd State Medical UniversityVolgograd State Medical University; Volgograd Regional Urology and Nephrology CentreVolgograd State Medical UniversityVolgograd State Medical University; Volgograd Regional Hospital No. 1Volgograd State Medical UniversityVolgograd State Medical UniversityIntroduction. For the treatment of overactive bladder syndrome (OAB), injection of botulinum toxin A (BoNT-A) has been shown to be effective. However, there is a need for a less invasive method for administering BoNT-A, which could significantly expand the treatment options for OAB.Objective. To assess the impact of tizol on the absorption of BoNT-A by the bladder mucosa and compare it to the individual absorption of BoNT-A.Materials & Methods. Dialysis through the mucous membrane of the сalf bladder was used as an experimental model to study changes in bioavailability of BoNT-A complexed with tisol (BoNT-A + T) and pure BoNT-A solution during in vitro experiment. After dialysis, the BoNT-A concentration in both samples was determined using a spectrophotometer. Dialysis curves were plotted according to the data obtained. Kruvchinsky equilibrium dialysis method was used to determine botulinum toxin A bioavailability. The UV spectrophotometry method was used to determine the concentration of BoNT-A in the acceptor medium by reaction of BoNT-A with Benedict's reagent.Results. It was established that the maximum concentration of BoNT-A diffused into the acceptor medium from the blend of the test substance with tizol after nine hours. The area under the curve for dialysis of BoNT-A + T exceeds the area under the curve of pure BoNT-A by almost 20%, suggesting an improvement in the drug's bioavailability when blended with tizol.Conclusion. Based on our experiment, it was found out that the BoNT-A + T has greater bioavailability than a solution of pure BoNT-A. However, the diffusion rate of the component mixture is sufficiently low.https://www.urovest.ru/jour/article/view/885botulinum toxin atizol, titanium glycerosolvateurotheliumhyperactive bladder |
| spellingShingle | S. V. Poroyskiy D. V. Perlin O. G. Srussovskaya N. A. Goncharov A. A. Kuznetsov E. A. Morozov Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study Вестник урологии botulinum toxin a tizol, titanium glycerosolvate urothelium hyperactive bladder |
| title | Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study |
| title_full | Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study |
| title_fullStr | Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study |
| title_full_unstemmed | Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study |
| title_short | Comparative evaluation of bioavailability of Botulinum toxin A complexed with Tizol (titanium glycerosolvate aquacomplex) versus pure Botulinum toxin A solution for bladder mucosa: an experimental study |
| title_sort | comparative evaluation of bioavailability of botulinum toxin a complexed with tizol titanium glycerosolvate aquacomplex versus pure botulinum toxin a solution for bladder mucosa an experimental study |
| topic | botulinum toxin a tizol, titanium glycerosolvate urothelium hyperactive bladder |
| url | https://www.urovest.ru/jour/article/view/885 |
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