Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars
Purpose: To describe a case of well-documented corneal stroma transparency improvement following the intrastromal implantation of autologous adipose-derived mesenchymal stem cells (ADASC) in a keratoconic patient with established stromal scars. Observations: ADASC were isolated by elective liposucti...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | American Journal of Ophthalmology Case Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2451993625000234 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823864438418046976 |
|---|---|
| author | Jorge L. Alio del Barrio Alberto Parafita-Fernandez Daniel Gomez Plaza Maria Eugenia Fernandez Jorge L. Alio |
| author_facet | Jorge L. Alio del Barrio Alberto Parafita-Fernandez Daniel Gomez Plaza Maria Eugenia Fernandez Jorge L. Alio |
| author_sort | Jorge L. Alio del Barrio |
| collection | DOAJ |
| description | Purpose: To describe a case of well-documented corneal stroma transparency improvement following the intrastromal implantation of autologous adipose-derived mesenchymal stem cells (ADASC) in a keratoconic patient with established stromal scars. Observations: ADASC were isolated by elective liposuction, and a solution composed of 3x106 ADASC contained in 1mL saline was used to soak a corneal intrastromal pocket created with femtosecond laser at mid-depth. No signs of inflammation or rejection were observed. One year after surgery, we observed a complete restoration of the pre-existing corneal stroma scars, observed both clinically and by anterior segment OCT. The rest of the visual and topographic parameters did not show relevant changes except for the patient's refractive sphere. OCT showed a thin new layer of neocollagen deposited at the surgical plane. Total stroma optical density (OD) improved from 51.5 to 41.2 GSU, anterior stroma OD improved from 55.9 to 42.8 GSU, and posterior stroma OD improved from 46.9 to 39.6 GSU. Conclusions and importance: This clinical case provides new clinical evidence supporting the use of intrastromal mesenchymal stem cell implantation to solve or alleviate established corneal scars. |
| format | Article |
| id | doaj-art-d6d6fa0f7d614021962b43dec1ada304 |
| institution | Kabale University |
| issn | 2451-9936 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | American Journal of Ophthalmology Case Reports |
| spelling | doaj-art-d6d6fa0f7d614021962b43dec1ada3042025-02-09T05:00:44ZengElsevierAmerican Journal of Ophthalmology Case Reports2451-99362025-03-0137102270Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scarsJorge L. Alio del Barrio0Alberto Parafita-Fernandez1Daniel Gomez Plaza2Maria Eugenia Fernandez3Jorge L. Alio4Cornea, Cataract and Refractive Surgery Unit, Vissum (Grupo Miranza), Alicante, Spain; Division of Ophthalmology, School of Medicine, Universidad Miguel Hernández, Alicante, SpainOphthalmology Department, Complexo Hospitalario Universitario de Pontevedra, Pontevedra, Spain; Clínica Oftalmológica Dr Parafita, Ribeira, A Coruña, SpainCornea, Cataract and Refractive Surgery Unit, Vissum (Grupo Miranza), Alicante, Spain; Instituto de Microcirugía Ocular (IMO), Barcelona, SpainUnidad de Terapia Celular. Hospital Gregorio Marañon, Madrid, SpainCornea, Cataract and Refractive Surgery Unit, Vissum (Grupo Miranza), Alicante, Spain; Division of Ophthalmology, School of Medicine, Universidad Miguel Hernández, Alicante, Spain; Corresponding author. Avda de Denia s/n, Vissum (Miranza Group), 03016, Alicante, Spain.Purpose: To describe a case of well-documented corneal stroma transparency improvement following the intrastromal implantation of autologous adipose-derived mesenchymal stem cells (ADASC) in a keratoconic patient with established stromal scars. Observations: ADASC were isolated by elective liposuction, and a solution composed of 3x106 ADASC contained in 1mL saline was used to soak a corneal intrastromal pocket created with femtosecond laser at mid-depth. No signs of inflammation or rejection were observed. One year after surgery, we observed a complete restoration of the pre-existing corneal stroma scars, observed both clinically and by anterior segment OCT. The rest of the visual and topographic parameters did not show relevant changes except for the patient's refractive sphere. OCT showed a thin new layer of neocollagen deposited at the surgical plane. Total stroma optical density (OD) improved from 51.5 to 41.2 GSU, anterior stroma OD improved from 55.9 to 42.8 GSU, and posterior stroma OD improved from 46.9 to 39.6 GSU. Conclusions and importance: This clinical case provides new clinical evidence supporting the use of intrastromal mesenchymal stem cell implantation to solve or alleviate established corneal scars.http://www.sciencedirect.com/science/article/pii/S2451993625000234Stem cellsCorneal transplantCorneaCellular therapyKeratoconusMesenchymal stem cells |
| spellingShingle | Jorge L. Alio del Barrio Alberto Parafita-Fernandez Daniel Gomez Plaza Maria Eugenia Fernandez Jorge L. Alio Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars American Journal of Ophthalmology Case Reports Stem cells Corneal transplant Cornea Cellular therapy Keratoconus Mesenchymal stem cells |
| title | Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars |
| title_full | Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars |
| title_fullStr | Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars |
| title_full_unstemmed | Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars |
| title_short | Intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars |
| title_sort | intrastromal autologous implantation of adipose derived adult stem cells for the management of established corneal scars |
| topic | Stem cells Corneal transplant Cornea Cellular therapy Keratoconus Mesenchymal stem cells |
| url | http://www.sciencedirect.com/science/article/pii/S2451993625000234 |
| work_keys_str_mv | AT jorgelaliodelbarrio intrastromalautologousimplantationofadiposederivedadultstemcellsforthemanagementofestablishedcornealscars AT albertoparafitafernandez intrastromalautologousimplantationofadiposederivedadultstemcellsforthemanagementofestablishedcornealscars AT danielgomezplaza intrastromalautologousimplantationofadiposederivedadultstemcellsforthemanagementofestablishedcornealscars AT mariaeugeniafernandez intrastromalautologousimplantationofadiposederivedadultstemcellsforthemanagementofestablishedcornealscars AT jorgelalio intrastromalautologousimplantationofadiposederivedadultstemcellsforthemanagementofestablishedcornealscars |