HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene
The present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/804167 |
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| author | Antonio C. R. Vallinoto Bárbara Brasil Santana Keyla S. G. Sá Tuane C. S. Ferreira Rita Catarina M. Sousa Vânia N. Azevedo Rosimar N. M. Feitosa Luiz Fernando A. Machado Marluísa O. G. Ishak Ricardo Ishak |
| author_facet | Antonio C. R. Vallinoto Bárbara Brasil Santana Keyla S. G. Sá Tuane C. S. Ferreira Rita Catarina M. Sousa Vânia N. Azevedo Rosimar N. M. Feitosa Luiz Fernando A. Machado Marluísa O. G. Ishak Ricardo Ishak |
| author_sort | Antonio C. R. Vallinoto |
| collection | DOAJ |
| description | The present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300 seronegative healthy controls were investigated. Plasma levels of the cytokines TNF-α, TNF-β, IL-8, IL-10, IL-6, and IFN-γ from infected patients were measured using an indirect enzyme-linked immunosorbent assay. The HTLV proviral load was measured using a real-time PCR assay, and T-cell subset counts were determined by flow cytometry. Real-time PCR was used to genotype the rs12979860 SNP. The allelic and genotypic distributions displayed no significant differences among the investigated groups. No significant association between the serum cytokine levels and the presence of the rs12979860 SNP in symptomatic and asymptomatic subjects was observed. A positive correlation (p=0.0015) between TNF-β and IFN-γ was observed in the asymptomatic group, but a positive correlation was only observed (p=0.0180) between TNF-α and IL-6 in the HAM/TSP group. The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects. The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome. |
| format | Article |
| id | doaj-art-d6d655a73d0c43e5bc93a16a3bb59a0b |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d6d655a73d0c43e5bc93a16a3bb59a0b2025-08-20T03:19:49ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/804167804167HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B GeneAntonio C. R. Vallinoto0Bárbara Brasil Santana1Keyla S. G. Sá2Tuane C. S. Ferreira3Rita Catarina M. Sousa4Vânia N. Azevedo5Rosimar N. M. Feitosa6Luiz Fernando A. Machado7Marluísa O. G. Ishak8Ricardo Ishak9Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilNúcleo de Medicina Tropical, Universidade Federal do Pará, Guamá, 66050-240 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilLaboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Guamá, 66075-110 Belém, PA, BrazilThe present study investigated the association between the rs12979860 polymorphism in the IL-28B gene and HTLV-1 infection as well as the development of HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1-infected patients (26 HAM/TSP symptomatic and 53 asymptomatic) and 300 seronegative healthy controls were investigated. Plasma levels of the cytokines TNF-α, TNF-β, IL-8, IL-10, IL-6, and IFN-γ from infected patients were measured using an indirect enzyme-linked immunosorbent assay. The HTLV proviral load was measured using a real-time PCR assay, and T-cell subset counts were determined by flow cytometry. Real-time PCR was used to genotype the rs12979860 SNP. The allelic and genotypic distributions displayed no significant differences among the investigated groups. No significant association between the serum cytokine levels and the presence of the rs12979860 SNP in symptomatic and asymptomatic subjects was observed. A positive correlation (p=0.0015) between TNF-β and IFN-γ was observed in the asymptomatic group, but a positive correlation was only observed (p=0.0180) between TNF-α and IL-6 in the HAM/TSP group. The proviral load was significantly higher in HAM/TSP patients than in asymptomatic subjects. The present results do not support a previous report indicating an association between the SNP rs12979860 and HAM/TSP outcome.http://dx.doi.org/10.1155/2015/804167 |
| spellingShingle | Antonio C. R. Vallinoto Bárbara Brasil Santana Keyla S. G. Sá Tuane C. S. Ferreira Rita Catarina M. Sousa Vânia N. Azevedo Rosimar N. M. Feitosa Luiz Fernando A. Machado Marluísa O. G. Ishak Ricardo Ishak HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene Mediators of Inflammation |
| title | HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene |
| title_full | HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene |
| title_fullStr | HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene |
| title_full_unstemmed | HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene |
| title_short | HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis Is Not Associated with SNP rs12979860 of the IL-28B Gene |
| title_sort | htlv 1 associated myelopathy tropical spastic paraparesis is not associated with snp rs12979860 of the il 28b gene |
| url | http://dx.doi.org/10.1155/2015/804167 |
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