BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis
Abstract Gray mold, caused by Botrytis cinerea, poses an escalating threat to ginseng production in China. Excessive application of chemical fungicides has resulted in severe resistance development. To elucidate the resistance mechanism of B. cinerea to pyrimethanil, transcriptomic comparisons were...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-96041-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849765523872546816 |
|---|---|
| author | Shuqi Wang Xinyi Zhang Shan Zhong Rong Wang Bingwei Chen Wanlong Ding Hongmei Luo Yong Li |
| author_facet | Shuqi Wang Xinyi Zhang Shan Zhong Rong Wang Bingwei Chen Wanlong Ding Hongmei Luo Yong Li |
| author_sort | Shuqi Wang |
| collection | DOAJ |
| description | Abstract Gray mold, caused by Botrytis cinerea, poses an escalating threat to ginseng production in China. Excessive application of chemical fungicides has resulted in severe resistance development. To elucidate the resistance mechanism of B. cinerea to pyrimethanil, transcriptomic comparisons were conducted between resistant (HRG21) and sensitive (FSG43) strains following exposure to pyrimethanil for 2 and 6 h. GO and KEGG analyses identified differential expression of genes associated with ABC and MFS transporters as well as methionine biosynthesis. qRT-PCR validation confirmed a marked upregulation of ABC (BcatrA, BcatrB, BcatrD, BcatrO, and Bmr3) and MFS (Bchex1 and BcmfsM2) transporter genes in HRG21, whereas in FSG43, ABC (BcatrA, BcatrB, BcatrD, and BcatrO) and MFS (Bchex1) transporter genes were downregulated. No significant transcriptional changes were observed in Mrr1, a transcription factor gene, following pyrimethanil exposure. Molecular docking analysis identified potential binding sites and interaction targets for five key transporters (BcatrB, BcatrA, BcatrD, Bmr3, and Bchex1). Furthermore, exogenous methionine supplementation partially mitigated pyrimethanil toxicity in FSG43. However, amino acid sequences of enzymes involved in methionine biosynthesis (BcmetC, BcStr2, Bcmet2, Bcmxr1, and Bcmxr2) exhibited no variation between FSG43 and HRG21. The results indicate that BcatrB plays a central role in pyrimethanil resistance, while methionine contributes only marginally to resistance mechanisms. |
| format | Article |
| id | doaj-art-d6caa4158d54485887321534a09cea55 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d6caa4158d54485887321534a09cea552025-08-20T03:04:49ZengNature PortfolioScientific Reports2045-23222025-04-0115111410.1038/s41598-025-96041-3BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysisShuqi Wang0Xinyi Zhang1Shan Zhong2Rong Wang3Bingwei Chen4Wanlong Ding5Hongmei Luo6Yong Li7State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Chinese Academy of Medical Sciences, Peking Union Medical College, Institute of Medicinal Plant DevelopmentAbstract Gray mold, caused by Botrytis cinerea, poses an escalating threat to ginseng production in China. Excessive application of chemical fungicides has resulted in severe resistance development. To elucidate the resistance mechanism of B. cinerea to pyrimethanil, transcriptomic comparisons were conducted between resistant (HRG21) and sensitive (FSG43) strains following exposure to pyrimethanil for 2 and 6 h. GO and KEGG analyses identified differential expression of genes associated with ABC and MFS transporters as well as methionine biosynthesis. qRT-PCR validation confirmed a marked upregulation of ABC (BcatrA, BcatrB, BcatrD, BcatrO, and Bmr3) and MFS (Bchex1 and BcmfsM2) transporter genes in HRG21, whereas in FSG43, ABC (BcatrA, BcatrB, BcatrD, and BcatrO) and MFS (Bchex1) transporter genes were downregulated. No significant transcriptional changes were observed in Mrr1, a transcription factor gene, following pyrimethanil exposure. Molecular docking analysis identified potential binding sites and interaction targets for five key transporters (BcatrB, BcatrA, BcatrD, Bmr3, and Bchex1). Furthermore, exogenous methionine supplementation partially mitigated pyrimethanil toxicity in FSG43. However, amino acid sequences of enzymes involved in methionine biosynthesis (BcmetC, BcStr2, Bcmet2, Bcmxr1, and Bcmxr2) exhibited no variation between FSG43 and HRG21. The results indicate that BcatrB plays a central role in pyrimethanil resistance, while methionine contributes only marginally to resistance mechanisms.https://doi.org/10.1038/s41598-025-96041-3Botrytis cinereaPyrimethanilResistance mechanismsEfflux transportersMethionine biosynthesis |
| spellingShingle | Shuqi Wang Xinyi Zhang Shan Zhong Rong Wang Bingwei Chen Wanlong Ding Hongmei Luo Yong Li BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis Scientific Reports Botrytis cinerea Pyrimethanil Resistance mechanisms Efflux transporters Methionine biosynthesis |
| title | BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis |
| title_full | BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis |
| title_fullStr | BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis |
| title_full_unstemmed | BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis |
| title_short | BcatrB mediates pyrimethanil resistance in Botrytis cinerea revealed by transcriptomics analysis |
| title_sort | bcatrb mediates pyrimethanil resistance in botrytis cinerea revealed by transcriptomics analysis |
| topic | Botrytis cinerea Pyrimethanil Resistance mechanisms Efflux transporters Methionine biosynthesis |
| url | https://doi.org/10.1038/s41598-025-96041-3 |
| work_keys_str_mv | AT shuqiwang bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT xinyizhang bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT shanzhong bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT rongwang bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT bingweichen bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT wanlongding bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT hongmeiluo bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis AT yongli bcatrbmediatespyrimethanilresistanceinbotrytiscinerearevealedbytranscriptomicsanalysis |