Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023
We used metagenomic next-generation sequencing (mNGS) to investigate an outbreak of Fusarium solani meningitis in US patients who had surgical procedures under spinal anesthesia in Matamoros, Mexico, during 2023. Using a novel method called metaMELT (metagenomic multiple extended locus typing), we...
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Centers for Disease Control and Prevention
2025-05-01
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| Series: | Emerging Infectious Diseases |
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| Online Access: | https://wwwnc.cdc.gov/eid/article/31/5/24-1657_article |
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| author | Charles Y. Chiu Venice Servellita Mikael de Lorenzi-Tognon Patrick Benoit Nanami Sumimoto Abiodun Foresythe Filipe M. Cerqueira Natalie Williams-Bouyer Ping Ren Lauren Nicholas S. Herrera David C. Gaston Leanna Sayyad Shannon L. Whitmer John Klena Holenarasipur R. Vikram Jeremy A.W. Gold Lalitha Gade Lindsay Parnell Elizabeth Misas Tom M. Chiller Isabel S. Griffin Sridhar V. Basavaraju Dallas J. Smith Anastasia P. Litvintseva Nancy A. Chow |
| author_facet | Charles Y. Chiu Venice Servellita Mikael de Lorenzi-Tognon Patrick Benoit Nanami Sumimoto Abiodun Foresythe Filipe M. Cerqueira Natalie Williams-Bouyer Ping Ren Lauren Nicholas S. Herrera David C. Gaston Leanna Sayyad Shannon L. Whitmer John Klena Holenarasipur R. Vikram Jeremy A.W. Gold Lalitha Gade Lindsay Parnell Elizabeth Misas Tom M. Chiller Isabel S. Griffin Sridhar V. Basavaraju Dallas J. Smith Anastasia P. Litvintseva Nancy A. Chow |
| author_sort | Charles Y. Chiu |
| collection | DOAJ |
| description |
We used metagenomic next-generation sequencing (mNGS) to investigate an outbreak of Fusarium solani meningitis in US patients who had surgical procedures under spinal anesthesia in Matamoros, Mexico, during 2023. Using a novel method called metaMELT (metagenomic multiple extended locus typing), we performed phylogenetic analysis of concatenated mNGS reads from 4 patients (P1–P4) in parallel with reads from 28 fungal reference genomes. Fungal strains from the 4 patients were most closely related to each other and to 2 cultured isolates from P1 and an additional case (P5), suggesting that all cases arose from a point source exposure. Our findings support epidemiologic data implicating a contaminated drug or device used for epidural anesthesia as the likely cause of the outbreak. In addition, our findings show that the benefits of mNGS extend beyond diagnosis of infections to public health outbreak investigation.
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| format | Article |
| id | doaj-art-d6c7bae6ca14435fb9a7a44fc22d1d28 |
| institution | DOAJ |
| issn | 1080-6040 1080-6059 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj-art-d6c7bae6ca14435fb9a7a44fc22d1d282025-08-20T03:13:48ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592025-05-0131594895710.3201/eid3105.241657Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023Charles Y. ChiuVenice ServellitaMikael de Lorenzi-TognonPatrick BenoitNanami SumimotoAbiodun ForesytheFilipe M. CerqueiraNatalie Williams-BouyerPing RenLauren Nicholas S. HerreraDavid C. GastonLeanna SayyadShannon L. WhitmerJohn KlenaHolenarasipur R. VikramJeremy A.W. GoldLalitha GadeLindsay ParnellElizabeth MisasTom M. ChillerIsabel S. GriffinSridhar V. BasavarajuDallas J. SmithAnastasia P. LitvintsevaNancy A. Chow We used metagenomic next-generation sequencing (mNGS) to investigate an outbreak of Fusarium solani meningitis in US patients who had surgical procedures under spinal anesthesia in Matamoros, Mexico, during 2023. Using a novel method called metaMELT (metagenomic multiple extended locus typing), we performed phylogenetic analysis of concatenated mNGS reads from 4 patients (P1–P4) in parallel with reads from 28 fungal reference genomes. Fungal strains from the 4 patients were most closely related to each other and to 2 cultured isolates from P1 and an additional case (P5), suggesting that all cases arose from a point source exposure. Our findings support epidemiologic data implicating a contaminated drug or device used for epidural anesthesia as the likely cause of the outbreak. In addition, our findings show that the benefits of mNGS extend beyond diagnosis of infections to public health outbreak investigation. https://wwwnc.cdc.gov/eid/article/31/5/24-1657_articleFusarium solanifungimeningitis/encephalitismetaMELTmultiple extended locus typingmetagenomic next-generation sequencing |
| spellingShingle | Charles Y. Chiu Venice Servellita Mikael de Lorenzi-Tognon Patrick Benoit Nanami Sumimoto Abiodun Foresythe Filipe M. Cerqueira Natalie Williams-Bouyer Ping Ren Lauren Nicholas S. Herrera David C. Gaston Leanna Sayyad Shannon L. Whitmer John Klena Holenarasipur R. Vikram Jeremy A.W. Gold Lalitha Gade Lindsay Parnell Elizabeth Misas Tom M. Chiller Isabel S. Griffin Sridhar V. Basavaraju Dallas J. Smith Anastasia P. Litvintseva Nancy A. Chow Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023 Emerging Infectious Diseases Fusarium solani fungi meningitis/encephalitis metaMELT multiple extended locus typing metagenomic next-generation sequencing |
| title | Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023 |
| title_full | Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023 |
| title_fullStr | Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023 |
| title_full_unstemmed | Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023 |
| title_short | Metagenomic Identification of Fusarium solani Strain as Cause of US Fungal Meningitis Outbreak Associated with Surgical Procedures in Mexico, 2023 |
| title_sort | metagenomic identification of fusarium solani strain as cause of us fungal meningitis outbreak associated with surgical procedures in mexico 2023 |
| topic | Fusarium solani fungi meningitis/encephalitis metaMELT multiple extended locus typing metagenomic next-generation sequencing |
| url | https://wwwnc.cdc.gov/eid/article/31/5/24-1657_article |
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