A mixed-mode LC-MS-based method for comprehensive analysis of NAD and related metabolites from biological sample matrices

A mixed-mode LC-MS-based method for comprehensive analysis of NAD and related metabolites from biological sample matrices

Abstract Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite contributing to cellular energy needs and its decline is associated with age-related disorders. Comprehensive analysis of the NAD+ landscape following NAD+ supplementation therapies would provide a broader understanding of...

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Bibliographic Details
Main Authors: Omprakash Nacham, Jordan W. Brown, Mohammad Mehdi Maneshi, Victoria Kurschner, Mike Sheehan, Renee Sadowski, Chris Ling, Nari Talaty, Robert Johnson, Andrew M. Swensen
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Scientific Reports
Subjects:
Nicotinamide adenine dinucleotide
Nicotinamide
NAD-Glo
Targeted mass spectrometry
Mixed mode chromatography
NADomics
Online Access:https://doi.org/10.1038/s41598-025-97834-2
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