Microfluidic device for both active and passive cell separation techniques: A review

Microfluidic separation of particles and cells has been extensively developed in recent years in a wide range of applications of sciences, engineering, and industry. At the same time, this area has been explored within the framework of interdisciplinary study, resulting in several experiments conduc...

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Main Authors: Muhammad Asraf Mansor, Muhammad Asyraf Jamrus, Chong Kar Lok, Mohd Ridzuan Ahmad, Michal Petrů, Seyed Saeid Rahimian Koloor
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Sensors and Actuators Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666053924000936
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author Muhammad Asraf Mansor
Muhammad Asyraf Jamrus
Chong Kar Lok
Mohd Ridzuan Ahmad
Michal Petrů
Seyed Saeid Rahimian Koloor
author_facet Muhammad Asraf Mansor
Muhammad Asyraf Jamrus
Chong Kar Lok
Mohd Ridzuan Ahmad
Michal Petrů
Seyed Saeid Rahimian Koloor
author_sort Muhammad Asraf Mansor
collection DOAJ
description Microfluidic separation of particles and cells has been extensively developed in recent years in a wide range of applications of sciences, engineering, and industry. At the same time, this area has been explored within the framework of interdisciplinary study, resulting in several experiments conducted in this field. Due to the heterogeneity of cells, label-free methods that utilize the inherent physical properties of cells to separate, offer more advantages including high throughput, simple design, reduced sample volumes, and low device cost. This paper comprehensively studies the latest progress in microfluidic technology for both active and passive cell separation techniques. In order to limit the extent of this work, our primary emphasis is on six common methods of cell separation: acoustophoresis, dielectrophoresis, magnetophoresis, inertial, pinched flow fractionation (PFF), and deterministic lateral displacement (DLD). We elaborate on the efficiency, throughput rate, and clogging in microfluidic devices. In addition, the advantages and limitations of both microfluidics devices were discussed.
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spelling doaj-art-d6ae34375248449fb3c93dd687c9ebbe2025-08-20T02:35:24ZengElsevierSensors and Actuators Reports2666-05392025-06-01910027710.1016/j.snr.2024.100277Microfluidic device for both active and passive cell separation techniques: A reviewMuhammad Asraf Mansor0Muhammad Asyraf Jamrus1Chong Kar Lok2Mohd Ridzuan Ahmad3Michal Petrů4Seyed Saeid Rahimian Koloor5Department of Control and Mechatronics Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, MalaysiaDepartment of Control and Mechatronics Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, MalaysiaDepartment of Control and Mechatronics Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, MalaysiaDepartment of Control and Mechatronics Engineering, Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia; Corresponding authors.Department of Mechanical Engineering, Technical University of Liberec, Liberec, Czech RepublicInstitute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Liberec, Czech Republic; Corresponding authors.Microfluidic separation of particles and cells has been extensively developed in recent years in a wide range of applications of sciences, engineering, and industry. At the same time, this area has been explored within the framework of interdisciplinary study, resulting in several experiments conducted in this field. Due to the heterogeneity of cells, label-free methods that utilize the inherent physical properties of cells to separate, offer more advantages including high throughput, simple design, reduced sample volumes, and low device cost. This paper comprehensively studies the latest progress in microfluidic technology for both active and passive cell separation techniques. In order to limit the extent of this work, our primary emphasis is on six common methods of cell separation: acoustophoresis, dielectrophoresis, magnetophoresis, inertial, pinched flow fractionation (PFF), and deterministic lateral displacement (DLD). We elaborate on the efficiency, throughput rate, and clogging in microfluidic devices. In addition, the advantages and limitations of both microfluidics devices were discussed.http://www.sciencedirect.com/science/article/pii/S2666053924000936Cell separationMicrofluidicActive cell separationPassive cell separation
spellingShingle Muhammad Asraf Mansor
Muhammad Asyraf Jamrus
Chong Kar Lok
Mohd Ridzuan Ahmad
Michal Petrů
Seyed Saeid Rahimian Koloor
Microfluidic device for both active and passive cell separation techniques: A review
Sensors and Actuators Reports
Cell separation
Microfluidic
Active cell separation
Passive cell separation
title Microfluidic device for both active and passive cell separation techniques: A review
title_full Microfluidic device for both active and passive cell separation techniques: A review
title_fullStr Microfluidic device for both active and passive cell separation techniques: A review
title_full_unstemmed Microfluidic device for both active and passive cell separation techniques: A review
title_short Microfluidic device for both active and passive cell separation techniques: A review
title_sort microfluidic device for both active and passive cell separation techniques a review
topic Cell separation
Microfluidic
Active cell separation
Passive cell separation
url http://www.sciencedirect.com/science/article/pii/S2666053924000936
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