Acute dietary methionine restriction triggers cell cycle arrest and reversible growth defects in the neocortex
Summary: Methionine is indispensable for cell proliferation, stem cell maintenance, and epigenetic regulation, three processes that are central to embryonic development. Here, we assessed the consequences of short-term dietary methionine restriction (MR) on mouse embryonic organ growth. In compariso...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225009666 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Summary: Methionine is indispensable for cell proliferation, stem cell maintenance, and epigenetic regulation, three processes that are central to embryonic development. Here, we assessed the consequences of short-term dietary methionine restriction (MR) on mouse embryonic organ growth. In comparison with growth of the liver and heart which was unaffected, MR for 5 days led to a severe reduction in neuronal production and neocortex growth. Progenitor cohort labeling revealed a time-dependent sensitivity to MR and cell cycle analysis indicated that progenitors are stalled in S/G2 phases following MR. Unexpectedly, neuronal production was completely rescued at birth when switching the dam back to control diet, uncovering a mechanism of catch-up growth. We used imaging mass cytometry to probe metabolic and epigenetic markers in neural progenitors following MR and during catch-up growth. Altogether, our data uncover a reversible state of quiescence in S/G2 which is metabolically distinct from G0 quiescence and associated with efficient catch-up growth of the neocortex. |
|---|---|
| ISSN: | 2589-0042 |