Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin
Pomacea canaliculata is one of the most destructive threats to the safe production of rice. Molluscicides are the primary measure for controlling this pest species. Tea saponin (TS) is a plant secondary metabolite with a high snail-killing activity. However, some individuals have enhanced detoxifica...
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Elsevier
2025-03-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325003872 |
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| author | Yanhui Lu Haitao Lu Xusong Zheng Hongxing Xu Zhongxian Lu |
| author_facet | Yanhui Lu Haitao Lu Xusong Zheng Hongxing Xu Zhongxian Lu |
| author_sort | Yanhui Lu |
| collection | DOAJ |
| description | Pomacea canaliculata is one of the most destructive threats to the safe production of rice. Molluscicides are the primary measure for controlling this pest species. Tea saponin (TS) is a plant secondary metabolite with a high snail-killing activity. However, some individuals have enhanced detoxification metabolism against TS, leading to a weakened control efficiency of TS to P. canaliculata. To clarify the detoxification and metabolic mechanisms of TS in P. canaliculata, we characterized the toxicity of TS against the snails. Additionally, we examined its biochemical and molecular effects on glutathione S-transferases (GSTs) and monoamine oxidase (MAO) activities, as well as their corresponding gene expression levels. The results showed that the LC50 values were 6.12, 9.03, and 9.35 mg/L for small, medium, and large snails following 24 h exposure. The LT50 values were 16.85, 20.96, and 29.85 h for small, medium, and large snails at a concentration of 9 mg/L exposure, respectively. Furthermore, as the TS concentration increased or the treatment duration was prolonged, the activities of GSTs and MAO increased, along with the corresponding gene expression levels of PcGSTs1, PcGSTo1, PcmGST1 and PcMAO, compared to those of untreated snails. These results indicated that TS displayed dose- and time-dependent toxicity to P. canaliculata. Overall, the biochemical and molecular results suggested that GSTs and MAO were probably involved in the detoxification metabolism of P. canaliculata against TS. These findings preliminarily clarified the detoxification mechanism of P. canaliculata against TS. |
| format | Article |
| id | doaj-art-d6a2146c0cb94cb6bdc458cffc3af610 |
| institution | OA Journals |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-d6a2146c0cb94cb6bdc458cffc3af6102025-08-20T01:49:28ZengElsevierEcotoxicology and Environmental Safety0147-65132025-03-0129311805110.1016/j.ecoenv.2025.118051Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponinYanhui Lu0Haitao Lu1Xusong Zheng2Hongxing Xu3Zhongxian Lu4State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCorresponding author.; State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaPomacea canaliculata is one of the most destructive threats to the safe production of rice. Molluscicides are the primary measure for controlling this pest species. Tea saponin (TS) is a plant secondary metabolite with a high snail-killing activity. However, some individuals have enhanced detoxification metabolism against TS, leading to a weakened control efficiency of TS to P. canaliculata. To clarify the detoxification and metabolic mechanisms of TS in P. canaliculata, we characterized the toxicity of TS against the snails. Additionally, we examined its biochemical and molecular effects on glutathione S-transferases (GSTs) and monoamine oxidase (MAO) activities, as well as their corresponding gene expression levels. The results showed that the LC50 values were 6.12, 9.03, and 9.35 mg/L for small, medium, and large snails following 24 h exposure. The LT50 values were 16.85, 20.96, and 29.85 h for small, medium, and large snails at a concentration of 9 mg/L exposure, respectively. Furthermore, as the TS concentration increased or the treatment duration was prolonged, the activities of GSTs and MAO increased, along with the corresponding gene expression levels of PcGSTs1, PcGSTo1, PcmGST1 and PcMAO, compared to those of untreated snails. These results indicated that TS displayed dose- and time-dependent toxicity to P. canaliculata. Overall, the biochemical and molecular results suggested that GSTs and MAO were probably involved in the detoxification metabolism of P. canaliculata against TS. These findings preliminarily clarified the detoxification mechanism of P. canaliculata against TS.http://www.sciencedirect.com/science/article/pii/S0147651325003872SaponinSnailGSTsMAODetoxification |
| spellingShingle | Yanhui Lu Haitao Lu Xusong Zheng Hongxing Xu Zhongxian Lu Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin Ecotoxicology and Environmental Safety Saponin Snail GSTs MAO Detoxification |
| title | Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin |
| title_full | Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin |
| title_fullStr | Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin |
| title_full_unstemmed | Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin |
| title_short | Role of glutathione S-transferases and monoamine oxidase in the detoxification of Pomacea canaliculata exposed to tea saponin |
| title_sort | role of glutathione s transferases and monoamine oxidase in the detoxification of pomacea canaliculata exposed to tea saponin |
| topic | Saponin Snail GSTs MAO Detoxification |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325003872 |
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