Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis
Pseudorabies virus (PRV) is a pathogen that causes severe neurological dysfunction in the host, leading to extensive neuronal damage and inflammation. Despite extensive research on the neuropathogenesis of α-herpesvirus infections, many scientific questions remain unresolved, such as the largely unk...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/4/580 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849714055561871360 |
|---|---|
| author | Yanwei Li Teng Tu Yan Luo Xueping Yao Zexiao Yang Yin Wang |
| author_facet | Yanwei Li Teng Tu Yan Luo Xueping Yao Zexiao Yang Yin Wang |
| author_sort | Yanwei Li |
| collection | DOAJ |
| description | Pseudorabies virus (PRV) is a pathogen that causes severe neurological dysfunction in the host, leading to extensive neuronal damage and inflammation. Despite extensive research on the neuropathogenesis of α-herpesvirus infections, many scientific questions remain unresolved, such as the largely unknown functions of long non-coding RNAs (lncRNAs) in herpesvirus-infected nervous systems. To address these questions, we used RNA sequencing (RNA-seq) to investigate the expression profiles of lncRNAs and mRNAs in the brains of mice infected with PRV. Through bioinformatic analysis, we identified 316 differentially expressed lncRNAs and 886 differentially expressed mRNAs. We predicted the biological functions of these differentially expressed lncRNAs and mRNAs using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and the results showed that the differentially expressed transcripts were mainly involved in the innate immune response. Finally, we validated the differential expression trends of lncRNAs and mRNAs using quantitative real-time PCR (q-PCR), which were consistent with the sequencing data. To our knowledge, this is the first report analyzing the lncRNA expression profile in the central nervous system (CNS) of mice infected with PRV. Our findings provide new insights into the roles of lncRNAs and mRNAs during PRV infection of the host CNS. |
| format | Article |
| id | doaj-art-d697b50cd71043de83f6046fe013f1fb |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-d697b50cd71043de83f6046fe013f1fb2025-08-20T03:13:48ZengMDPI AGViruses1999-49152025-04-0117458010.3390/v17040580Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic AnalysisYanwei Li0Teng Tu1Yan Luo2Xueping Yao3Zexiao Yang4Yin Wang5Key Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaKey Laboratory of Animal Diseases and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, ChinaPseudorabies virus (PRV) is a pathogen that causes severe neurological dysfunction in the host, leading to extensive neuronal damage and inflammation. Despite extensive research on the neuropathogenesis of α-herpesvirus infections, many scientific questions remain unresolved, such as the largely unknown functions of long non-coding RNAs (lncRNAs) in herpesvirus-infected nervous systems. To address these questions, we used RNA sequencing (RNA-seq) to investigate the expression profiles of lncRNAs and mRNAs in the brains of mice infected with PRV. Through bioinformatic analysis, we identified 316 differentially expressed lncRNAs and 886 differentially expressed mRNAs. We predicted the biological functions of these differentially expressed lncRNAs and mRNAs using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, and the results showed that the differentially expressed transcripts were mainly involved in the innate immune response. Finally, we validated the differential expression trends of lncRNAs and mRNAs using quantitative real-time PCR (q-PCR), which were consistent with the sequencing data. To our knowledge, this is the first report analyzing the lncRNA expression profile in the central nervous system (CNS) of mice infected with PRV. Our findings provide new insights into the roles of lncRNAs and mRNAs during PRV infection of the host CNS.https://www.mdpi.com/1999-4915/17/4/580pseudorabies virusRNA-seqlncRNAbrain |
| spellingShingle | Yanwei Li Teng Tu Yan Luo Xueping Yao Zexiao Yang Yin Wang Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis Viruses pseudorabies virus RNA-seq lncRNA brain |
| title | Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis |
| title_full | Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis |
| title_fullStr | Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis |
| title_full_unstemmed | Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis |
| title_short | Expression Profiles of lncRNAs and mRNAs in the Mouse Brain Infected with Pseudorabies Virus: A Bioinformatic Analysis |
| title_sort | expression profiles of lncrnas and mrnas in the mouse brain infected with pseudorabies virus a bioinformatic analysis |
| topic | pseudorabies virus RNA-seq lncRNA brain |
| url | https://www.mdpi.com/1999-4915/17/4/580 |
| work_keys_str_mv | AT yanweili expressionprofilesoflncrnasandmrnasinthemousebraininfectedwithpseudorabiesvirusabioinformaticanalysis AT tengtu expressionprofilesoflncrnasandmrnasinthemousebraininfectedwithpseudorabiesvirusabioinformaticanalysis AT yanluo expressionprofilesoflncrnasandmrnasinthemousebraininfectedwithpseudorabiesvirusabioinformaticanalysis AT xuepingyao expressionprofilesoflncrnasandmrnasinthemousebraininfectedwithpseudorabiesvirusabioinformaticanalysis AT zexiaoyang expressionprofilesoflncrnasandmrnasinthemousebraininfectedwithpseudorabiesvirusabioinformaticanalysis AT yinwang expressionprofilesoflncrnasandmrnasinthemousebraininfectedwithpseudorabiesvirusabioinformaticanalysis |