Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity.
Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle p...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051559&type=printable |
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| author | Irina Margine Luis Martinez-Gil Yi-Ying Chou Florian Krammer |
| author_facet | Irina Margine Luis Martinez-Gil Yi-Ying Chou Florian Krammer |
| author_sort | Irina Margine |
| collection | DOAJ |
| description | Influenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle preparations generated in the two systems. Our work shows significant differences in immunogenicity between the two, and indicates superior and broader immune responses induced by the baculovirus-derived constructs. We demonstrate that these differences critically influence protection and survival in a mouse model of influenza virus infection. Finally, we show that the enhanced immunogenicity of the baculovirus-derived virus-like particles is caused by contamination with residual baculovirus which activates the innate immune response at the site of inoculation. |
| format | Article |
| id | doaj-art-d697720e876f4d0184e3a8979441ba8a |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-d697720e876f4d0184e3a8979441ba8a2025-08-20T03:09:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5155910.1371/journal.pone.0051559Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity.Irina MargineLuis Martinez-GilYi-Ying ChouFlorian KrammerInfluenza virus-like particles are currently evaluated in clinical trials as vaccine candidates for influenza viruses. Most commonly they are produced in baculovirus- or mammalian- expression systems. Here we used different vaccination schemes in order to systematically compare virus-like particle preparations generated in the two systems. Our work shows significant differences in immunogenicity between the two, and indicates superior and broader immune responses induced by the baculovirus-derived constructs. We demonstrate that these differences critically influence protection and survival in a mouse model of influenza virus infection. Finally, we show that the enhanced immunogenicity of the baculovirus-derived virus-like particles is caused by contamination with residual baculovirus which activates the innate immune response at the site of inoculation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051559&type=printable |
| spellingShingle | Irina Margine Luis Martinez-Gil Yi-Ying Chou Florian Krammer Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. PLoS ONE |
| title | Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. |
| title_full | Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. |
| title_fullStr | Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. |
| title_full_unstemmed | Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. |
| title_short | Residual baculovirus in insect cell-derived influenza virus-like particle preparations enhances immunogenicity. |
| title_sort | residual baculovirus in insect cell derived influenza virus like particle preparations enhances immunogenicity |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051559&type=printable |
| work_keys_str_mv | AT irinamargine residualbaculovirusininsectcellderivedinfluenzaviruslikeparticlepreparationsenhancesimmunogenicity AT luismartinezgil residualbaculovirusininsectcellderivedinfluenzaviruslikeparticlepreparationsenhancesimmunogenicity AT yiyingchou residualbaculovirusininsectcellderivedinfluenzaviruslikeparticlepreparationsenhancesimmunogenicity AT floriankrammer residualbaculovirusininsectcellderivedinfluenzaviruslikeparticlepreparationsenhancesimmunogenicity |