Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites
The biocompatibility and bioactivity properties of hydroxyapatites (HAs) modified through lithium addition were investigated. Hydroxyapatites obtained from bovine bone were mixed with lithium carbonate (Li), in the proportions of 0.25, 0.50, 1.00, and 2.00% wt, and sintered at 900°, 1000°, 1100°, 12...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2012/650574 |
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| author | Ana Paula M. Shainberg Patrícia Valério Alessandra Zonari Faik N. Oktar Lutfiye S. Ozyegin Manuel Pedro F. Graça Maria F. Leite Alfredo M. Goes |
| author_facet | Ana Paula M. Shainberg Patrícia Valério Alessandra Zonari Faik N. Oktar Lutfiye S. Ozyegin Manuel Pedro F. Graça Maria F. Leite Alfredo M. Goes |
| author_sort | Ana Paula M. Shainberg |
| collection | DOAJ |
| description | The biocompatibility and bioactivity properties of hydroxyapatites (HAs) modified through lithium addition were investigated. Hydroxyapatites obtained from bovine bone were mixed with lithium carbonate (Li), in the proportions of 0.25, 0.50, 1.00, and 2.00% wt, and sintered at 900°, 1000°, 1100°, 1200°, and 1300°C, creating LiHA samples. The osteoblast culture behavior was assessed in the presence of these LiHA compositions. The cellular interactions were analyzed by evaluating the viability and cellular proliferation, ALP production and collagen secretion. The cytotoxic potential was investigated through measurement of apoptosis and necrosis induction. The process of cellular attachment in the presence of the product of dissolution of LiHA, was evaluated trough fluorescence analysis. The physical characteristics of these materials and their cellular interactions were examined with SEM and EDS. The results of this study indicate that the LiHA ceramics are biocompatible and have variable bioactivities, which can be tailored by different combinations of the concentration of lithium carbonate and the sintering temperature. Our findings suggest that LiHA 0.25% wt, sintered at 1300°C, combines the necessary physical and structural qualities with favorable biocompatibility characteristics, achieving a bioactivity that seems to be adequate for use as a bone implant material. |
| format | Article |
| id | doaj-art-d696b3e927f348da9f7d7edb3593d008 |
| institution | OA Journals |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-d696b3e927f348da9f7d7edb3593d0082025-08-20T02:19:33ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422012-01-01201210.1155/2012/650574650574Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite CompositesAna Paula M. Shainberg0Patrícia Valério1Alessandra Zonari2Faik N. Oktar3Lutfiye S. Ozyegin4Manuel Pedro F. Graça5Maria F. Leite6Alfredo M. Goes7Department of Morphology, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Biochemistry and Immunology, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Biochemistry and Immunology, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Bioengineering, Faculty of Engineering, Marmara University, Goztepe Campus, Kadikoy, 34722 Istanbul, TurkeyMarmara University, Goztepe Campus, Kadikoy, 34722 Istanbul, TurkeyI3N-Aveiro-Physic Department, University of Aveiro, 3810-193 Aveiro, PortugalDepartment of Physiology and Biophysics, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilDepartment of Morphology, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, BrazilThe biocompatibility and bioactivity properties of hydroxyapatites (HAs) modified through lithium addition were investigated. Hydroxyapatites obtained from bovine bone were mixed with lithium carbonate (Li), in the proportions of 0.25, 0.50, 1.00, and 2.00% wt, and sintered at 900°, 1000°, 1100°, 1200°, and 1300°C, creating LiHA samples. The osteoblast culture behavior was assessed in the presence of these LiHA compositions. The cellular interactions were analyzed by evaluating the viability and cellular proliferation, ALP production and collagen secretion. The cytotoxic potential was investigated through measurement of apoptosis and necrosis induction. The process of cellular attachment in the presence of the product of dissolution of LiHA, was evaluated trough fluorescence analysis. The physical characteristics of these materials and their cellular interactions were examined with SEM and EDS. The results of this study indicate that the LiHA ceramics are biocompatible and have variable bioactivities, which can be tailored by different combinations of the concentration of lithium carbonate and the sintering temperature. Our findings suggest that LiHA 0.25% wt, sintered at 1300°C, combines the necessary physical and structural qualities with favorable biocompatibility characteristics, achieving a bioactivity that seems to be adequate for use as a bone implant material.http://dx.doi.org/10.1155/2012/650574 |
| spellingShingle | Ana Paula M. Shainberg Patrícia Valério Alessandra Zonari Faik N. Oktar Lutfiye S. Ozyegin Manuel Pedro F. Graça Maria F. Leite Alfredo M. Goes Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites Advances in Materials Science and Engineering |
| title | Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites |
| title_full | Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites |
| title_fullStr | Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites |
| title_full_unstemmed | Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites |
| title_short | Attachment and Proliferation of Osteoblasts on Lithium-Hydroxyapatite Composites |
| title_sort | attachment and proliferation of osteoblasts on lithium hydroxyapatite composites |
| url | http://dx.doi.org/10.1155/2012/650574 |
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