Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil.
<h4>Background</h4>Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sampl...
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033016&type=printable |
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| author | Ivano de Filippis Ana Paula S de Lemos Jessica B Hostetler Kurt Wollenberg Claudio T Sacchi Julie C Dunning Hotopp Lee H Harrison Margaret C Bash D Rebecca Prevots |
| author_facet | Ivano de Filippis Ana Paula S de Lemos Jessica B Hostetler Kurt Wollenberg Claudio T Sacchi Julie C Dunning Hotopp Lee H Harrison Margaret C Bash D Rebecca Prevots |
| author_sort | Ivano de Filippis |
| collection | DOAJ |
| description | <h4>Background</h4>Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988-2006) for study (n = 372).<h4>Methods</h4>We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA.<h4>Results</h4>In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1.<h4>Conclusions</h4>A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp. |
| format | Article |
| id | doaj-art-d68f31a1791d4f2291cbb15848a4ca24 |
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| issn | 1932-6203 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-d68f31a1791d4f2291cbb15848a4ca242025-08-20T02:30:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0173e3301610.1371/journal.pone.0033016Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil.Ivano de FilippisAna Paula S de LemosJessica B HostetlerKurt WollenbergClaudio T SacchiJulie C Dunning HotoppLee H HarrisonMargaret C BashD Rebecca Prevots<h4>Background</h4>Neisseria meningitidis serogroup B has been predominant in Brazil, but no broadly effective vaccine is available to prevent endemic meningococcal disease. To understand genetic diversity among serogroup B strains in Brazil, we selected a nationally representative sample of clinical disease isolates from 2004, and a temporally representative sample for the state of São Paulo (1988-2006) for study (n = 372).<h4>Methods</h4>We performed multi-locus sequence typing (MLST) and sequence analysis of five outer membrane protein (OMP) genes, including novel vaccine targets fHbp and nadA.<h4>Results</h4>In 2004, strain B:4:P1.15,19 clonal complex ST-32/ET-5 (cc32) predominated throughout Brazil; regional variation in MLST sequence type (ST), fetA, and porB was significant but diversity was limited for nadA and fHbp. Between 1988 and 1996, the São Paulo isolates shifted from clonal complex ST-41/44/Lineage 3 (cc41/44) to cc32. OMP variation was associated with but not predicted by cc or ST. Overall, fHbp variant 1/subfamily B was present in 80% of isolates and showed little diversity. The majority of nadA were similar to reference allele 1.<h4>Conclusions</h4>A predominant serogroup B lineage has circulated in Brazil for over a decade with significant regional and temporal diversity in ST, fetA, and porB, but not in nadA and fHbp.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033016&type=printable |
| spellingShingle | Ivano de Filippis Ana Paula S de Lemos Jessica B Hostetler Kurt Wollenberg Claudio T Sacchi Julie C Dunning Hotopp Lee H Harrison Margaret C Bash D Rebecca Prevots Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. PLoS ONE |
| title | Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. |
| title_full | Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. |
| title_fullStr | Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. |
| title_full_unstemmed | Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. |
| title_short | Molecular epidemiology of Neisseria meningitidis serogroup B in Brazil. |
| title_sort | molecular epidemiology of neisseria meningitidis serogroup b in brazil |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0033016&type=printable |
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